Fig. 4.

EA altered the balance of mitochondrial dynamics in cerebral I/R injury. (A–F) Western blot and quantitative analysis of Drp1 and Fis1, Mfn2, Mfn2 and Opa1 (n = 5). GAPDH was used as an internal control. The experiments were repeated three times in each group. (G–J) Immunofluorescence staining and quantitative analysis of Drp1 and Opa1 (n = 5). The nuclei were stained with DAPI (blue). The target proteins were stained green. The representative positive cells were marked with red arrows. Drp1: dynamin-related protein 1; Fis1; fission 1; Mfn1: mitofusin 1; Mfn1: mitofusin 2; Opa1: optic atrophy 1. All data are shown as mean ± SD. Statistical significance was determined using one-way ANOVA followed by LSD post hoc test. ** indicates P < 0.01, compared with sham group; ns indicates not significant, ## indicates P < 0.01, compared with model group. The original versions of Fig. 4A are presented as supplementary data. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)