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[Preprint]. 2025 May 15:2024.08.11.607360. Originally published 2024 Aug 11. [Version 3] doi: 10.1101/2024.08.11.607360

Translation efficiency covariation across cell types is a conserved organizing principle of mammalian transcriptomes

Yue Liu, Shilpa Rao, Ian Hoskins, Michael Geng, Qiuxia Zhao, Jonathan Chacko, Vighnesh Ghatpande, Kangsheng Qi, Logan Persyn, Jun Wang, Dinghai Zheng, Yochen Zhong, Dayea Park, Elif Sarinay Cenik, Vikram Agarwal, Hakan Ozadam, Can Cenik
PMCID: PMC11326257  PMID: 39149359

ABSTRACT

Characterizing shared patterns of RNA expression between genes across conditions has led to the discovery of regulatory networks and novel biological functions. However, it is unclear if such coordination extends to translation. Here, we uniformly analyzed 3,819 ribosome profiling datasets from 117 human and 94 mouse tissues and cell lines. We introduce the concept of translation efficiency covariation (TEC), identifying coordinated translation patterns across cell types. We nominate candidate mechanisms driving shared patterns of translation regulation. TEC is conserved across human and mouse cells and uncovers gene functions that are not evident from RNA or protein co-expression. Moreover, our observations indicate that proteins that physically interact are highly enriched for positive covariation at both translational and transcriptional levels. Our findings establish TEC as a conserved organizing principle of mammalian transcriptomes.

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