Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1992 Jun 1;284(Pt 2):441–445. doi: 10.1042/bj2840441

Maltose/proton co-transport in Saccharomyces cerevisiae. Comparative study with cells and plasma membrane vesicles.

C C Van Leeuwen 1, R A Weusthuis 1, E Postma 1, P J Van den Broek 1, J P Van Dijken 1
PMCID: PMC1132658  PMID: 1318030

Abstract

Maltose/proton co-transport was studied in intact cells and in plasma membrane vesicles of the yeast Saccharomyces cerevisiae. In order to determine uphill transport in vesicles, plasma membranes were fused with proteoliposomes containing cytochrome c oxidase as a proton-motive force-generating system. Maltose accumulation, dependent on the electrical and pH gradients, was observed. The initial uptake velocity and accumulation ratio in vesicles proved to be dependent on the external pH. Moreover, kinetic analysis of maltose transport showed that Vmax. values greatly decreased with increasing pH, whereas the Km remained virtually constant. These observations were in good agreement with results obtained with intact cells, and suggest that proton binding to the carrier proceeds with an apparent pK of 5.7. The observation with intact cells that maltose is co-transported with protons in a one-to-one stoichiometry was ascertained in the vesicle system by measuring the balance between proton-motive force and the chemical maltose gradient. These results show that maltose transport in vesicles prepared by fusion of plasma membranes with cytochrome c oxidase proteoliposomes behaves in a similar way as in intact cells. It is therefore concluded that this vesicle model system offers a wide range of new possibilities for the study of maltose/proton co-transport in more detail.

Full text

PDF
441

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calahorra M., Opekarová M., Ramirez J., Peña A. Leucine transport in plasma membrane vesicles of Saccharomyces cerevisiae. FEBS Lett. 1989 Apr 24;247(2):235–238. doi: 10.1016/0014-5793(89)81342-0. [DOI] [PubMed] [Google Scholar]
  2. Cheng Q., Michels C. A. MAL11 and MAL61 encode the inducible high-affinity maltose transporter of Saccharomyces cerevisiae. J Bacteriol. 1991 Mar;173(5):1817–1820. doi: 10.1128/jb.173.5.1817-1820.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clement N. R., Gould J. M. Pyranine (8-hydroxy-1,3,6-pyrenetrisulfonate) as a probe of internal aqueous hydrogen ion concentration in phospholipid vesicles. Biochemistry. 1981 Mar 17;20(6):1534–1538. doi: 10.1021/bi00509a019. [DOI] [PubMed] [Google Scholar]
  4. Driessen A. J., Hellingwerf K. J., Konings W. N. Mechanism of energy coupling to entry and exit of neutral and branched chain amino acids in membrane vesicles of Streptococcus cremoris. J Biol Chem. 1987 Sep 15;262(26):12438–12443. [PubMed] [Google Scholar]
  5. Driessen A. J., de Vrij W., Konings W. N. Functional incorporation of beef-heart cytochrome c oxidase into membranes of Streptococcus cremoris. Eur J Biochem. 1986 Feb 3;154(3):617–624. doi: 10.1111/j.1432-1033.1986.tb09443.x. [DOI] [PubMed] [Google Scholar]
  6. Driessen A. J., de Vrij W., Konings W. N. Incorporation of beef heart cytochrome c oxidase as a proton-motive force-generating mechanism in bacterial membrane vesicles. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7555–7559. doi: 10.1073/pnas.82.22.7555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Franzusoff A. J., Cirillo V. P. Glucose transport activity in isolated plasma membrane vesicles from Saccharomyces cerevisiae. J Biol Chem. 1983 Mar 25;258(6):3608–3614. [PubMed] [Google Scholar]
  8. Görts C. P. Effect of glucose on the activity and the kinetics of the maltose-uptake system and of alpha-glucosidase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1969 Jul 30;184(2):299–305. doi: 10.1016/0304-4165(69)90032-4. [DOI] [PubMed] [Google Scholar]
  9. Hoekstra D., de Boer T., Klappe K., Wilschut J. Fluorescence method for measuring the kinetics of fusion between biological membranes. Biochemistry. 1984 Nov 20;23(24):5675–5681. doi: 10.1021/bi00319a002. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Needleman R. B., Federoff H. J., Eccleshall T. R., Buchferer B., Marmur J. Purification and characterization of an alpha-glucosidase from Saccharomyces carlsbergensis. Biochemistry. 1978 Oct 31;17(22):4657–4661. doi: 10.1021/bi00615a011. [DOI] [PubMed] [Google Scholar]
  12. Ongjoco R., Szkutnicka K., Cirillo V. P. Glucose transport in vesicles reconstituted from Saccharomyces cerevisiae membranes and liposomes. J Bacteriol. 1987 Jul;169(7):2926–2931. doi: 10.1128/jb.169.7.2926-2931.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Postma E., Van den Broek P. J. Continuous-culture study of the regulation of glucose and fructose transport in Kluyveromyces marxianus CBS 6556. J Bacteriol. 1990 Jun;172(6):2871–2876. doi: 10.1128/jb.172.6.2871-2876.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Postma E., Verduyn C., Kuiper A., Scheffers W. A., van Dijken J. P. Substrate-accelerated death of Saccharomyces cerevisiae CBS 8066 under maltose stress. Yeast. 1990 Mar-Apr;6(2):149–158. doi: 10.1002/yea.320060209. [DOI] [PubMed] [Google Scholar]
  15. Ramos J., Szkutnicka K., Cirillo V. P. Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles. J Bacteriol. 1989 Jun;171(6):3539–3544. doi: 10.1128/jb.171.6.3539-3544.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Seaston A., Inkson C., Eddy A. A. The absorption of protons with specific amino acids and carbohydrates by yeast. Biochem J. 1973 Aug;134(4):1031–1043. doi: 10.1042/bj1341031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Serrano R. Energy requirements for maltose transport in yeast. Eur J Biochem. 1977 Oct 17;80(1):97–102. doi: 10.1111/j.1432-1033.1977.tb11861.x. [DOI] [PubMed] [Google Scholar]
  18. Shinbo T., Kamo N., Kurihara K., Kobatake Y. A PVC-based electrode sensitive to DDA+ as a device for monitoring the membrane potential in biological systems. Arch Biochem Biophys. 1978 Apr 30;187(2):414–422. doi: 10.1016/0003-9861(78)90052-8. [DOI] [PubMed] [Google Scholar]
  19. Van Leeuwen C. C., Postma E., Van den Broek P. J., Van Steveninck J. Proton-motive force-driven D-galactose transport in plasma membrane vesicles from the yeast Kluyveromyces marxianus. J Biol Chem. 1991 Jul 5;266(19):12146–12151. [PubMed] [Google Scholar]
  20. Van den Broek P. J., Van Steveninck J. Kinetic analysis of H+/methyl beta-D-thiogalactoside symport in Saccharomyces fragilis. Biochim Biophys Acta. 1982 Dec 8;693(1):213–220. doi: 10.1016/0005-2736(82)90489-8. [DOI] [PubMed] [Google Scholar]
  21. Viitanen P., Newman M. J., Foster D. L., Wilson T. H., Kaback H. R. Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Methods Enzymol. 1986;125:429–452. doi: 10.1016/s0076-6879(86)25034-x. [DOI] [PubMed] [Google Scholar]
  22. YONETANI T. Studies on cytochrome oxidase. III. Improved preparation and some properties. J Biol Chem. 1961 Jun;236:1680–1688. [PubMed] [Google Scholar]
  23. Yu C., Yu L., King T. E. Studies on cytochrome oxidase. Interactions of the cytochrome oxidase protein with phospholipids and cytochrome c. J Biol Chem. 1975 Feb 25;250(4):1383–1392. [PubMed] [Google Scholar]
  24. de Vrij W., Driessen A. J., Hellingwerf K. J., Konings W. N. Measurements of the proton motive force generated by cytochrome c oxidase from Bacillus subtilis in proteoliposomes and membrane vesicles. Eur J Biochem. 1986 Apr 15;156(2):431–440. doi: 10.1111/j.1432-1033.1986.tb09600.x. [DOI] [PubMed] [Google Scholar]
  25. de Vrij W., van den Burg B., Konings W. N. Spectral and potentiometric analysis of cytochromes from Bacillus subtilis. Eur J Biochem. 1987 Aug 3;166(3):589–595. doi: 10.1111/j.1432-1033.1987.tb13554.x. [DOI] [PubMed] [Google Scholar]
  26. van Urk H., Postma E., Scheffers W. A., van Dijken J. P. Glucose transport in crabtree-positive and crabtree-negative yeasts. J Gen Microbiol. 1989 Sep;135(9):2399–2406. doi: 10.1099/00221287-135-9-2399. [DOI] [PubMed] [Google Scholar]
  27. van den Broek P. J., van Steveninck J. Kinetic analysis of simultaneously occurring proton-sorbose symport and passive sorbose transport in Saccharomyces fragilis. Biochim Biophys Acta. 1980 Nov 4;602(2):419–432. doi: 10.1016/0005-2736(80)90321-1. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES