Abstract
The structure of a novel diphosphodiester compound recently detected in Desulfovibrio desulfuricans cells [Santos, Fareleira, Pedregal, LeGall & Xavier (1991) Eur. J. Biochem. 201, 283-287] was fully elucidated using a combination of n.m.r. techniques in aqueous and in methanolic solutions. The novel metabolite was identified as 3-methyl-1,2,3,4-tetrahydroxybutane-1,3-cyclic bisphosphate, and the minimum energy conformation is presented. The two chiral centres have the relative configuration RS.
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Selected References
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- Kjeldstad B., Johnsson A., Furuheim K. M., Bergan A. S., Krane J. Hyperthermia induced polyphosphate changes in Propionibacterium acnes as studied by 31P NMR. Z Naturforsch C. 1989 Jan-Feb;44(1-2):45–48. [PubMed] [Google Scholar]
- Kumar A., Ernst R. R., Wüthrich K. A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules. Biochem Biophys Res Commun. 1980 Jul 16;95(1):1–6. doi: 10.1016/0006-291x(80)90695-6. [DOI] [PubMed] [Google Scholar]
- Lee C. H., Sarma R. H. Aqueous solution conformation of rigid nucleosides and nucleotides. J Am Chem Soc. 1976 Jun 9;98(12):3541–3548. doi: 10.1021/ja00428a026. [DOI] [PubMed] [Google Scholar]
- Ostrovsky D., Shipanova I., Sibeldina L., Shashkov A., Kharatian E., Malyarova I., Tantsyrev G. A new cyclopyrophosphate as a bacterial antistressor? FEBS Lett. 1992 Feb 24;298(2-3):159–161. doi: 10.1016/0014-5793(92)80045-i. [DOI] [PubMed] [Google Scholar]
- Santos H., Fareleira P., Pedregal C., LeGall J., Xavier A. V. In vivo 31P-NMR studies of Desulfovibrio species. Detection of a novel phosphorus-containing compound. Eur J Biochem. 1991 Oct 1;201(1):283–287. doi: 10.1111/j.1432-1033.1991.tb16285.x. [DOI] [PubMed] [Google Scholar]