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. 1992 Jul 15;285(Pt 2):597–601. doi: 10.1042/bj2850597

Activation of protein kinase C is not an absolute requirement for amylase release from permeabilized rat pancreatic acini.

A J O'Sullivan 1, J D Jamieson 1
PMCID: PMC1132830  PMID: 1379047

Abstract

The effect of protein kinase C (PKC) on amylase discharge from streptolysin-O-permeabilized rat pancreatic acini was investigated. Addition of phorbol 12-myristate 13-acetate (PMA) to permeabilized cells potentiated Ca(2+)-stimulated release, but had no effect on discharge at non-stimulatory Ca2+ concentrations. PMA markedly shifted the Ca(2+)-concentration-dependence of amylase discharge to the left, by enhancing the time over which the permeabilized cells release. This effect was inhibited by both staurosporine and PKC-19-31-amide peptide inhibitor, indicating that the effect of PMA was due to its action on PKC. Staurosporine also partially inhibited amylase release at the optimal concentration of Ca2+; this effect was not replicated by the more specific PKC-19-31-amide peptide inhibitor and may be due to an effect on another second-messenger system. PKC appears to be an important modulator of release in pancreatic acini, but its activation is not an absolute requirement for Ca(2+)-dependent amylase discharge.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali S. M., Geisow M. J., Burgoyne R. D. A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells. Nature. 1989 Jul 27;340(6231):313–315. doi: 10.1038/340313a0. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J., Irvine R. F. Inositol phosphates and cell signalling. Nature. 1989 Sep 21;341(6239):197–205. doi: 10.1038/341197a0. [DOI] [PubMed] [Google Scholar]
  3. Bruzzone R., Halban P. A., Gjinovci A., Trimble E. R. A new, rapid, method for preparation of dispersed pancreatic acini. Biochem J. 1985 Mar 1;226(2):621–624. doi: 10.1042/bj2260621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burwen S. J., Rothman S. S. Zymogen granules: osmotic properties, interactions with ions, and some structural implications. Am J Physiol. 1972 May;222(5):1177–1181. doi: 10.1152/ajplegacy.1972.222.5.1177. [DOI] [PubMed] [Google Scholar]
  5. Churcher Y., Gomperts B. D. ATP-dependent and ATP-independent pathways of exocytosis revealed by interchanging glutamate and chloride as the major anion in permeabilized mast cells. Cell Regul. 1990 Mar;1(4):337–346. doi: 10.1091/mbc.1.4.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ederveen A. G., Van Emst-De Vries S. E., de Pont J. J., Willems P. H. Dissimilar effects of the protein kinase C inhibitors, staurosporine and H-7, on cholecystokinin-induced enzyme secretion from rabbit pancreatic acini. Eur J Biochem. 1990 Oct 5;193(1):291–295. doi: 10.1111/j.1432-1033.1990.tb19335.x. [DOI] [PubMed] [Google Scholar]
  7. Edwardson J. M., Vickery C., Christy L. J. Rat pancreatic acini permeabilised with streptolysin O secrete amylase at Ca2+ concentrations in the micromolar range, when provided with ATP and GTP gamma S. Biochim Biophys Acta. 1990 Jun 12;1053(1):32–36. doi: 10.1016/0167-4889(90)90022-6. [DOI] [PubMed] [Google Scholar]
  8. Fabiato A., Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 1979;75(5):463–505. [PubMed] [Google Scholar]
  9. Fallon R. J. Staurosporine inhibits a tyrosine protein kinase in human hepatoma cell membranes. Biochem Biophys Res Commun. 1990 Aug 16;170(3):1191–1196. doi: 10.1016/0006-291x(90)90519-s. [DOI] [PubMed] [Google Scholar]
  10. Fuller C. M., Deetjen H. H., Piiper A., Schulz I. Secretagogue and second messenger-activated Cl- permeabilities in isolated pancreatic zymogen granules. Pflugers Arch. 1989 Oct;415(1):29–36. doi: 10.1007/BF00373138. [DOI] [PubMed] [Google Scholar]
  11. HOKIN L. E. Isolation of the zymogen granules of dog pancreas and a study of their properties. Biochim Biophys Acta. 1955 Nov;18(3):379–388. doi: 10.1016/0006-3002(55)90101-3. [DOI] [PubMed] [Google Scholar]
  12. HOLTZER R. L., VAN LANCKER J. L., SWIFT H. Release of amylase from zymogen granules and microsomes. Arch Biochem Biophys. 1963 Jun;101:439–444. doi: 10.1016/0003-9861(63)90500-9. [DOI] [PubMed] [Google Scholar]
  13. House C., Kemp B. E. Protein kinase C contains a pseudosubstrate prototope in its regulatory domain. Science. 1987 Dec 18;238(4834):1726–1728. doi: 10.1126/science.3686012. [DOI] [PubMed] [Google Scholar]
  14. Jena B. P., Padfield P. J., Ingebritsen T. S., Jamieson J. D. Protein tyrosine phosphatase stimulates Ca(2+)-dependent amylase secretion from pancreatic acini. J Biol Chem. 1991 Sep 25;266(27):17744–17746. [PubMed] [Google Scholar]
  15. Kasai H., Augustine G. J. Cytosolic Ca2+ gradients triggering unidirectional fluid secretion from exocrine pancreas. Nature. 1990 Dec 20;348(6303):735–738. doi: 10.1038/348735a0. [DOI] [PubMed] [Google Scholar]
  16. Kitagawa M., Williams J. A., De Lisle R. C. Amylase release from streptolysin O-permeabilized pancreatic acini. Am J Physiol. 1990 Aug;259(2 Pt 1):G157–G164. doi: 10.1152/ajpgi.1990.259.2.G157. [DOI] [PubMed] [Google Scholar]
  17. Kitagawa M., Williams J. A., De Lisle R. C. Interactions of intracellular mediators of amylase secretion in permeabilized pancreatic acini. Biochim Biophys Acta. 1991 Jan 23;1073(1):129–135. doi: 10.1016/0304-4165(91)90192-j. [DOI] [PubMed] [Google Scholar]
  18. Knight D. E., Scrutton M. C. Gaining access to the cytosol: the technique and some applications of electropermeabilization. Biochem J. 1986 Mar 15;234(3):497–506. doi: 10.1042/bj2340497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kocher M., Clemetson K. J. Staurosporine both activates and inhibits serine/threonine kinases in human platelets. Biochem J. 1991 Apr 15;275(Pt 2):301–306. doi: 10.1042/bj2750301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morgan A., Burgoyne R. D. Relationship between arachidonic acid release and Ca2(+)-dependent exocytosis in digitonin-permeabilized bovine adrenal chromaffin cells. Biochem J. 1990 Nov 1;271(3):571–574. doi: 10.1042/bj2710571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nishizuka Y. Studies and prospectives of the protein kinase c family for cellular regulation. Cancer. 1989 May 15;63(10):1892–1903. doi: 10.1002/1097-0142(19890515)63:10<1892::aid-cncr2820631005>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  22. Padfield P. J., Ding T. G., Jamieson J. D. Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins. Biochem Biophys Res Commun. 1991 Jan 31;174(2):536–541. doi: 10.1016/0006-291x(91)91450-q. [DOI] [PubMed] [Google Scholar]
  23. Pandol S. J., Hsu Y. L., Kondratenko N. F., Schoeffield-Payne M. S., Steinbach J. H. Dual pathways for agonist-stimulated arachidonic acid release in pancreatic acini: roles in secretion. Am J Physiol. 1991 Mar;260(3 Pt 1):G423–G433. doi: 10.1152/ajpgi.1991.260.3.G423. [DOI] [PubMed] [Google Scholar]
  24. Pandol S. J., Schoeffield M. S. 1,2-Diacylglycerol, protein kinase C, and pancreatic enzyme secretion. J Biol Chem. 1986 Apr 5;261(10):4438–4444. [PubMed] [Google Scholar]
  25. Rüegg U. T., Burgess G. M. Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases. Trends Pharmacol Sci. 1989 Jun;10(6):218–220. doi: 10.1016/0165-6147(89)90263-0. [DOI] [PubMed] [Google Scholar]
  26. Sarafian T., Pradel L. A., Henry J. P., Aunis D., Bader M. F. The participation of annexin II (calpactin I) in calcium-evoked exocytosis requires protein kinase C. J Cell Biol. 1991 Sep;114(6):1135–1147. doi: 10.1083/jcb.114.6.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tamaoki T., Nomoto H., Takahashi I., Kato Y., Morimoto M., Tomita F. Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase. Biochem Biophys Res Commun. 1986 Mar 13;135(2):397–402. doi: 10.1016/0006-291x(86)90008-2. [DOI] [PubMed] [Google Scholar]
  28. Verme T. B., Velarde R. T., Cunningham R. M., Hootman S. R. Effects of staurosporine on protein kinase C and amylase secretion from pancreatic acini. Am J Physiol. 1989 Oct;257(4 Pt 1):G548–G553. doi: 10.1152/ajpgi.1989.257.4.G548. [DOI] [PubMed] [Google Scholar]
  29. Wu Y. N., Wagner P. D. Calpactin-depleted cytosolic proteins restore Ca(2+)-dependent secretion to digitonin-permeabilized bovine chromaffin cells. FEBS Lett. 1991 Apr 22;282(1):197–199. doi: 10.1016/0014-5793(91)80476-j. [DOI] [PubMed] [Google Scholar]

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