Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1992 Aug 15;286(Pt 1):55–63. doi: 10.1042/bj2860055

Substrate specificity of the bovine and feline neutral alpha-mannosidases.

R De Gasperi 1, S al Daher 1, B G Winchester 1, C D Warren 1
PMCID: PMC1133017  PMID: 1520284

Abstract

Neutral alpha-mannosidases were prepared from bovine and cat liver. The activities were distinguished from lysosomal and Golgi alpha-mannosidases by their neutral pH optima, relatively low Km for their synthetic substrate p-nitrophenyl alpha-D-mannoside, inhibition by Zn2+ and absence of inhibition by Co2+, EDTA, low concentrations of swainsonine, or deoxymannojirimycin. The cytosolic alpha-mannosidases were not retained by concanavalin A-Sepharose. They were able to degrade efficiently a variety of oligosaccharides with structures corresponding to certain high-mannose glycans or the oligomannosyl parts of hybrid and complex glycans. However, unlike lysosomal alpha-mannosidases from the same species these enzymes were not able to degrade Man9GlcNAc2 efficiently, and the bovine neutral alpha-mannosidase was not able to degrade a hexasaccharide with a structure analogous to Man5GlcNAc2-PP-dolichol. Sharp differences were noted for the bovine and cat enzymes with regard to the specificity of degradation. The bovine neutral alpha-mannosidase degraded the substrates by defined pathways, but the cat neutral alpha-mannosidase often produced complex mixtures of products, especially from the larger oligosaccharides. Therefore the bovine enzyme resembled the rat and human cytosolic alpha-mannosidases, but the cat enzyme did not. The bovine and cat neutral alpha-mannosidases, unlike the corresponding lysosomal activities, did not show specificity for the hydrolysis of the (1----3)- and (1----6)-linked mannose residues in the N-linked glycan pentasaccharide core.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham D., Daniel P., Dell A., Oates J., Sidebotham R., Winchester B. Structural analysis of the major urinary oligosaccharides in feline alpha-mannosidosis. Biochem J. 1986 Feb 1;233(3):899–904. doi: 10.1042/bj2330899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aronson N. N., Jr, Kuranda M. J. Lysosomal degradation of Asn-linked glycoproteins. FASEB J. 1989 Dec;3(14):2615–2622. doi: 10.1096/fasebj.3.14.2531691. [DOI] [PubMed] [Google Scholar]
  3. Bischoff J., Kornfeld R. Evidence for an alpha-mannosidase in endoplasmic reticulum of rat liver. J Biol Chem. 1983 Jul 10;258(13):7907–7910. [PubMed] [Google Scholar]
  4. Bischoff J., Kornfeld R. The soluble form of rat liver alpha-mannosidase is immunologically related to the endoplasmic reticulum membrane alpha-mannosidase. J Biol Chem. 1986 Apr 5;261(10):4758–4765. [PubMed] [Google Scholar]
  5. Bischoff J., Liscum L., Kornfeld R. The use of 1-deoxymannojirimycin to evaluate the role of various alpha-mannosidases in oligosaccharide processing in intact cells. J Biol Chem. 1986 Apr 5;261(10):4766–4774. [PubMed] [Google Scholar]
  6. Brassart D., Baussant T., Wieruszeski J. M., Strecker G., Montreuil J., Michalski J. C. Catabolism of N-glycosylprotein glycans: evidence for a degradation pathway of sialylglyco-asparagines resulting from the combined action of the lysosomal aspartylglucosaminidase and endo-N-acetyl-beta-D-glucosaminidase. A 400-MHz 1H-NMR study. Eur J Biochem. 1987 Nov 16;169(1):131–136. doi: 10.1111/j.1432-1033.1987.tb13589.x. [DOI] [PubMed] [Google Scholar]
  7. Burditt L. J., Chotai K., Hirani S., Nugent P. G., Winchester B. G., Blakemore W. F. Biochemical studies on a case of feline mannosidosis. Biochem J. 1980 Sep 1;189(3):467–473. doi: 10.1042/bj1890467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carroll M., Dance N., Masson P. K., Robinson D., Winchester B. G. Human mannosidosis--the enzyme defect. Biochem Biophys Res Commun. 1972 Oct 17;49(2):579–583. doi: 10.1016/0006-291x(72)90450-0. [DOI] [PubMed] [Google Scholar]
  9. DeGasperi R., Li Y. T., Li S. C. Presence of two endo-beta-N-acetylglucosaminidases in human kidney. J Biol Chem. 1989 Jun 5;264(16):9329–9334. [PubMed] [Google Scholar]
  10. DeGasperi R., al Daher S., Daniel P. F., Winchester B. G., Jeanloz R. W., Warren C. D. The substrate specificity of bovine and feline lysosomal alpha-D-mannosidases in relation to alpha-mannosidosis. J Biol Chem. 1991 Sep 5;266(25):16556–16563. [PubMed] [Google Scholar]
  11. Desgasperi R., al Daher S., Hall N., Winchester B., Warren C. D. Specificity of lysosomal alpha-mannosidase for natural substrates. Biochem Soc Trans. 1989 Dec;17(6):1036–1037. doi: 10.1042/bst0171036. [DOI] [PubMed] [Google Scholar]
  12. Haeuw J. F., Strecker G., Wieruszeski J. M., Montreuil J., Michalski J. C. Etude comparée des catabolismes lysosomique et cytosolique des glycannes de type oligommannosidique. C R Acad Sci III. 1991;312(4):131–139. [PubMed] [Google Scholar]
  13. Kornfeld R., Kornfeld S. Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985;54:631–664. doi: 10.1146/annurev.bi.54.070185.003215. [DOI] [PubMed] [Google Scholar]
  14. Marsh C. A., Gourlay G. C. Evidence for a non-lysosomal alpha-mannosidase in rat liver homogenates. Biochim Biophys Acta. 1971 Apr 14;235(1):142–148. doi: 10.1016/0005-2744(71)90041-6. [DOI] [PubMed] [Google Scholar]
  15. Matsuura F., Nunez H. A., Grabowski G. A., Sweeley C. C. Structural studies of urinary oligosaccharides from patients with mannosidosis. Arch Biochem Biophys. 1981 Apr 1;207(2):337–352. doi: 10.1016/0003-9861(81)90041-2. [DOI] [PubMed] [Google Scholar]
  16. Mellis S. J., Baenziger J. U. Separation of neutral oligosaccharides by high-performance liquid chromatography. Anal Biochem. 1981 Jul 1;114(2):276–280. doi: 10.1016/0003-2697(81)90480-2. [DOI] [PubMed] [Google Scholar]
  17. Opheim D. J., Touster O. Lysosomal alpha-D-mannosidase of rat liver. Purification and comparison with the golgi and cytosolic alpha-D-mannosidases. J Biol Chem. 1978 Feb 25;253(4):1017–1023. [PubMed] [Google Scholar]
  18. Phillips N. C., Robinson D., Winchester B. G. Characterization of human liver alpha-D-mannosidase purified by affinity chromatography. Biochem J. 1976 Mar 1;153(3):579–587. doi: 10.1042/bj1530579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Phillips N. C., Robinson D., Winchester B. G., Jolly R. D. Mannosidosis in Angus cattle. The enzymic defect. Biochem J. 1974 Feb;137(2):363–371. doi: 10.1042/bj1370363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shoup V. A., Touster O. Purification and characterization of the alpha-D-mannosidase of rat liver cytosol. J Biol Chem. 1976 Jul 10;251(13):3845–3852. [PubMed] [Google Scholar]
  21. Tulsiani D. R., Touster O. Substrate specificities of rat kidney lysosomal and cytosolic alpha-D-mannosidases and effects of swainsonine suggest a role of the cytosolic enzyme in glycoprotein catabolism. J Biol Chem. 1987 May 15;262(14):6506–6514. [PubMed] [Google Scholar]
  22. Warren C. D., Alroy J., Bugge B., Daniel P. F., Raghavan S. S., Kolodny E. H., Lamar J. J., Jeanloz R. W. Oligosaccharides from placenta: early diagnosis of feline mannosidosis. FEBS Lett. 1986 Jan 20;195(1-2):247–252. doi: 10.1016/0014-5793(86)80169-7. [DOI] [PubMed] [Google Scholar]
  23. Warren C. D., Azaroff L. S., Bugge B., Jeanloz R. W., Daniel P. F., Alroy J. The accumulation of oligosaccharides in tissues and body fluids of cats with alpha-mannosidosis. Carbohydr Res. 1988 Sep 15;180(2):325–338. doi: 10.1016/0008-6215(88)80089-2. [DOI] [PubMed] [Google Scholar]
  24. Warren C. D., Daniel P. F., Bugge B., Evans J. E., James L. F., Jeanloz R. W. The structures of oligosaccharides excreted by sheep with swainsonine toxicosis. J Biol Chem. 1988 Oct 15;263(29):15041–15049. [PubMed] [Google Scholar]
  25. Warren C. D., Schmit A. S., Jeanloz R. W. Chromatographic separation of oligosaccharides from mannosidosis urine. Carbohydr Res. 1983 Jun 1;116(2):171–182. doi: 10.1016/0008-6215(83)88107-5. [DOI] [PubMed] [Google Scholar]
  26. Yamashita K., Tachibana Y., Mihara K., Okada S., Yabuuchi H., Kobata A. Urinary oligosaccharides of mannosidosis. J Biol Chem. 1980 Jun 10;255(11):5126–5133. [PubMed] [Google Scholar]
  27. al Daher S., De Gasperi R., Daniel P., Hirani S., Warren C., Winchester B. Substrate specificity of human liver neutral alpha-mannosidase. Biochem J. 1992 Aug 15;286(Pt 1):47–53. doi: 10.1042/bj2860047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. al Daher S., De Gasperi R., Daniel P., Hirani S., Warren C., Winchester B. Substrate specificity of human liver neutral alpha-mannosidase. Biochem J. 1992 Aug 15;286(Pt 1):47–53. doi: 10.1042/bj2860047. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES