Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1992 Aug 15;286(Pt 1):153–156. doi: 10.1042/bj2860153

Intrinsic factor receptor during fetal development of the human intestine.

H Schohn 1, J L Guéant 1, B Leheup 1, M Saunier 1, G Grignon 1, J P Nicolas 1
PMCID: PMC1133032  PMID: 1325778

Abstract

Intrinsic factor receptor activity was observed in mucosal homogenates from whole small intestine and colon of 10-19-week fetuses, whereas it was only detected in the distal part of the small intestine of a 25-week fetus. The receptor-intrinsic factor-cobalamin complex was eluted into the void-volume position when ileum mucosal extract was assayed for receptor activity by gel filtration after incubation with either fetal gastric extract or human gastric juice. The intrinsic-factor-binding capacity of intestinal mucosal extracts ranged from 2.6 to 30.5 fmol/mg and was correlated with the gestational age of six fetuses. The dissociation constant of the receptor for the intrinsic factor-cobalamin complex was estimated at 0.24-0.36 nM at pH 7.4. In conclusion, intrinsic-factor-receptor activity was detected in the whole intestine in 10-19-week fetuses, whereas it was only present in the distal ileum at the end of fetal development.

Full text

PDF
153

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dix C. J., Hassan I. F., Obray H. Y., Shah R., Wilson G. The transport of vitamin B12 through polarized monolayers of Caco-2 cells. Gastroenterology. 1990 May;98(5 Pt 1):1272–1279. doi: 10.1016/0016-5085(90)90344-z. [DOI] [PubMed] [Google Scholar]
  2. Grossowicz N., Jablonska M., Beyth Y. Vitamin B12-binding proteins in human amniotic fluid: an index to fetal maturity. Am J Obstet Gynecol. 1983 Jun 1;146(3):331–332. doi: 10.1016/0002-9378(83)90756-1. [DOI] [PubMed] [Google Scholar]
  3. Guéant J. L., Champigneulle B., Gaucher P., Nicolas J. P. Malabsorption of vitamin B12 in pancreatic insufficiency of the adult and of the child. Pancreas. 1990 Sep;5(5):559–567. doi: 10.1097/00006676-199009000-00011. [DOI] [PubMed] [Google Scholar]
  4. Guéant J. L., Gérard A., Monin B., Champigneulle B., Gérard H., Nicolas J. P. Radioautographic localisation of iodinated human intrinsic factor in the guinea pig ileum using electron microscopy. Gut. 1988 Oct;29(10):1370–1378. doi: 10.1136/gut.29.10.1370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guéant J. L., Hambaba L., Vidailhet M., Schaefer C., Wahlstedt V., Nicolas J. P. Concentration and physicochemical characterisation of unsaturated cobalamin binding proteins in amniotic fluid. Clin Chim Acta. 1989 May 15;181(2):151–161. doi: 10.1016/0009-8981(89)90182-4. [DOI] [PubMed] [Google Scholar]
  6. Guéant J. L., Jokinen O., Schohn H., Monin B., Nicolas J. P., Gräsbeck R. Purification of intrinsic factor receptor from pig ileum using as affinity medium human intrinsic factor covalently bound to Sepharose. Biochim Biophys Acta. 1989 Sep 15;992(3):281–288. doi: 10.1016/0304-4165(89)90086-x. [DOI] [PubMed] [Google Scholar]
  7. Guéant J. L., Masson C., Schohn H., Girr M., Saunier M., Nicolas J. P. Receptor-mediated endocytosis of the intrinsic factor-cobalamin complex in HT 29, a human colon carcinoma cell line. FEBS Lett. 1992 Feb 10;297(3):229–232. doi: 10.1016/0014-5793(92)80544-q. [DOI] [PubMed] [Google Scholar]
  8. Guéant J. L., Schohn H., Brulé H., Saunier M., Gräsbeck R., Nicolas J. P. Binding assay and physicochemical characteristics of solubilized intrinsic factor receptor in ileal mucosal homogenates using phenyl-Sepharose to separate the saturated receptor from free intrinsic factor. Biochim Biophys Acta. 1991 Apr 9;1073(3):614–618. doi: 10.1016/0304-4165(91)90238-c. [DOI] [PubMed] [Google Scholar]
  9. Hooper D. C., Alpers D. H., Burger R. L., Mehlman C. S., Allen R. H. Characterization of ileal vitamin B12 Binding using homogeneous human and hog intrinsic factors. J Clin Invest. 1973 Dec;52(12):3074–3083. doi: 10.1172/JCI107506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kouvonen I., Gräsbeck R. A simplified technique to isolate the porcine and human ileal intrinsic factor receptors and studies on their subunit structures. Biochem Biophys Res Commun. 1979 Jan 30;86(2):358–364. doi: 10.1016/0006-291x(79)90873-8. [DOI] [PubMed] [Google Scholar]
  11. Levine J. S., Nakane P. K., Allen R. H. Immunocytochemical localization of intrinsic factor--cobalamin bound to the guinea pig ileum in vivo. Gastroenterology. 1982 Feb;82(2):284–290. [PubMed] [Google Scholar]
  12. Marcoullis G., Gräsbeck R. Vitamin B12-binding proteins in human gastric mucosa. General pattern and demonstration of intrinsic factor isoproteins typical of mucosa. Scand J Clin Lab Invest. 1975 Jan;35(1):5–11. doi: 10.1080/00365517509067998. [DOI] [PubMed] [Google Scholar]
  13. Ramanujam K. S., Seetharam S., Ramasamy M., Seetharam B. Expression of cobalamin transport proteins and cobalamin transcytosis by colon adenocarcinoma cells. Am J Physiol. 1991 Mar;260(3 Pt 1):G416–G422. doi: 10.1152/ajpgi.1991.260.3.G416. [DOI] [PubMed] [Google Scholar]
  14. Rousset M. The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation. Biochimie. 1986 Sep;68(9):1035–1040. doi: 10.1016/s0300-9084(86)80177-8. [DOI] [PubMed] [Google Scholar]
  15. Schohn H., Guéant J. L., Girr M., Nexø E., Baricault L., Zweibaum A., Nicolas J. P. Synthesis and secretion of a cobalamin-binding protein by HT 29 cell line. Biochem J. 1991 Dec 1;280(Pt 2):427–430. doi: 10.1042/bj2800427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]
  17. Sonneborn D. W., Iwanik M. J., Hansen H. J. Vitamin B 12 binding proteins in the human embryo. Proc Soc Exp Biol Med. 1974 Feb;145(2):675–677. doi: 10.3181/00379727-145-37873. [DOI] [PubMed] [Google Scholar]
  18. Stenman U. H. Amniotic fluid vitamin B12-binding protein. Purification and characterization with isoelectric focusing and other techniques. Biochim Biophys Acta. 1974 Mar 14;342(1):173–184. doi: 10.1016/0005-2795(74)90119-6. [DOI] [PubMed] [Google Scholar]
  19. Triadou N., Zweibaum A. Maturation of sucrase-isomaltase complex in human fetal small and large intestine during gestation. Pediatr Res. 1985 Jan;19(1):136–138. doi: 10.1203/00006450-198501000-00035. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES