Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1992 Nov 1;287(Pt 3):755–759. doi: 10.1042/bj2870755

Evolutionary conservation of the dystrophin central rod domain.

T G Sherratt 1, T Vulliamy 1, P N Strong 1
PMCID: PMC1133072  PMID: 1280104

Abstract

Dystrophin cDNA fragments encoding the C-terminal repeats of the central rod region have been expressed as fusion proteins. The polyclonal antisera raised to the purified fusion proteins have been characterized and neither antiserum cross-reacted with dystrophin-related protein. Antisera detected dystrophin with molecular mass close to that of the human in all terrestrial vertebrates and amphibia studied. Experiments with antisera to the N-terminal region of the dystrophin rod confirmed that epitopes to the rod region were conserved during this evolutionary period and the length of this domain remained unaltered.

Full text

PDF
755

Images in this article

756Fig. 1.
on p.756
757Fig. 3.
on p.757
757Fig. 4.
on p.757

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arahata K., Beggs A. H., Honda H., Ito S., Ishiura S., Tsukahara T., Ishiguro T., Eguchi C., Orimo S., Arikawa E. Preservation of the C-terminus of dystrophin molecule in the skeletal muscle from Becker muscular dystrophy. J Neurol Sci. 1991 Feb;101(2):148–156. doi: 10.1016/0022-510x(91)90039-a. [DOI] [PubMed] [Google Scholar]
  2. Beggs A. H., Hoffman E. P., Snyder J. R., Arahata K., Specht L., Shapiro F., Angelini C., Sugita H., Kunkel L. M. Exploring the molecular basis for variability among patients with Becker muscular dystrophy: dystrophin gene and protein studies. Am J Hum Genet. 1991 Jul;49(1):54–67. [PMC free article] [PubMed] [Google Scholar]
  3. Bulman D. E., Murphy E. G., Zubrzycka-Gaarn E. E., Worton R. G., Ray P. N. Differentiation of Duchenne and Becker muscular dystrophy phenotypes with amino- and carboxy-terminal antisera specific for dystrophin. Am J Hum Genet. 1991 Feb;48(2):295–304. [PMC free article] [PubMed] [Google Scholar]
  4. Chang H. W., Bock E., Bonilla E. Dystrophin in electric organ of Torpedo californica homologous to that in human muscle. J Biol Chem. 1989 Dec 15;264(35):20831–20834. [PubMed] [Google Scholar]
  5. England S. B., Nicholson L. V., Johnson M. A., Forrest S. M., Love D. R., Zubrzycka-Gaarn E. E., Bulman D. E., Harris J. B., Davies K. E. Very mild muscular dystrophy associated with the deletion of 46% of dystrophin. Nature. 1990 Jan 11;343(6254):180–182. doi: 10.1038/343180a0. [DOI] [PubMed] [Google Scholar]
  6. Feener C. A., Koenig M., Kunkel L. M. Alternative splicing of human dystrophin mRNA generates isoforms at the carboxy terminus. Nature. 1989 Apr 6;338(6215):509–511. doi: 10.1038/338509a0. [DOI] [PubMed] [Google Scholar]
  7. Heckmatt J. Z., Moosa A., Hutson C., Maunder-Sewry C. A., Dubowitz V. Diagnostic needle muscle biopsy. A practical and reliable alternative to open biopsy. Arch Dis Child. 1984 Jun;59(6):528–532. doi: 10.1136/adc.59.6.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoffman E. P., Brown R. H., Jr, Kunkel L. M. Dystrophin: the protein product of the Duchenne muscular dystrophy locus. Cell. 1987 Dec 24;51(6):919–928. doi: 10.1016/0092-8674(87)90579-4. [DOI] [PubMed] [Google Scholar]
  9. Hoffman E. P., Fischbeck K. H., Brown R. H., Johnson M., Medori R., Loike J. D., Harris J. B., Waterston R., Brooke M., Specht L. Characterization of dystrophin in muscle-biopsy specimens from patients with Duchenne's or Becker's muscular dystrophy. N Engl J Med. 1988 May 26;318(21):1363–1368. doi: 10.1056/NEJM198805263182104. [DOI] [PubMed] [Google Scholar]
  10. Jasmin B. J., Cartaud A., Ludosky M. A., Changeux J. P., Cartaud J. Asymmetric distribution of dystrophin in developing and adult Torpedo marmorata electrocyte: evidence for its association with the acetylcholine receptor-rich membrane. Proc Natl Acad Sci U S A. 1990 May;87(10):3938–3941. doi: 10.1073/pnas.87.10.3938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Khurana T. S., Hoffman E. P., Kunkel L. M. Identification of a chromosome 6-encoded dystrophin-related protein. J Biol Chem. 1990 Oct 5;265(28):16717–16720. [PubMed] [Google Scholar]
  12. Koenig M., Beggs A. H., Moyer M., Scherpf S., Heindrich K., Bettecken T., Meng G., Müller C. R., Lindlöf M., Kaariainen H. The molecular basis for Duchenne versus Becker muscular dystrophy: correlation of severity with type of deletion. Am J Hum Genet. 1989 Oct;45(4):498–506. [PMC free article] [PubMed] [Google Scholar]
  13. Koenig M., Kunkel L. M. Detailed analysis of the repeat domain of dystrophin reveals four potential hinge segments that may confer flexibility. J Biol Chem. 1990 Mar 15;265(8):4560–4566. [PubMed] [Google Scholar]
  14. Koenig M., Monaco A. P., Kunkel L. M. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell. 1988 Apr 22;53(2):219–228. doi: 10.1016/0092-8674(88)90383-2. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lemaire C., Heilig R., Mandel J. L. The chicken dystrophin cDNA: striking conservation of the C-terminal coding and 3' untranslated regions between man and chicken. EMBO J. 1988 Dec 20;7(13):4157–4162. doi: 10.1002/j.1460-2075.1988.tb03311.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Liechti-Gallati S., Koenig M., Kunkel L. M., Frey D., Boltshauser E., Schneider V., Braga S., Moser H. Molecular deletion patterns in Duchenne and Becker type muscular dystrophy. Hum Genet. 1989 Mar;81(4):343–348. doi: 10.1007/BF00283688. [DOI] [PubMed] [Google Scholar]
  19. Love D. R., Hill D. F., Dickson G., Spurr N. K., Byth B. C., Marsden R. F., Walsh F. S., Edwards Y. H., Davies K. E. An autosomal transcript in skeletal muscle with homology to dystrophin. Nature. 1989 May 4;339(6219):55–58. doi: 10.1038/339055a0. [DOI] [PubMed] [Google Scholar]
  20. Love D. R., Morris G. E., Ellis J. M., Fairbrother U., Marsden R. F., Bloomfield J. F., Edwards Y. H., Slater C. P., Parry D. J., Davies K. E. Tissue distribution of the dystrophin-related gene product and expression in the mdx and dy mouse. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3243–3247. doi: 10.1073/pnas.88.8.3243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  22. Nicholson L. V., Davison K., Falkous G., Harwood C., O'Donnell E., Slater C. R., Harris J. B. Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody. J Neurol Sci. 1989 Dec;94(1-3):125–136. doi: 10.1016/0022-510x(89)90223-2. [DOI] [PubMed] [Google Scholar]
  23. Patel K., Voit T., Dunn M. J., Strong P. N., Dubowitz V. Dystrophin and nebulin in the muscular dystrophies. J Neurol Sci. 1988 Nov;87(2-3):315–326. doi: 10.1016/0022-510x(88)90256-0. [DOI] [PubMed] [Google Scholar]
  24. Program and abstracts volume: 41st annual meeting, The American Society of Human Genetics. Cincinnati, Ohio, October 16-20, 1990. Am J Hum Genet. 1990 Sep;47(3 Suppl):A1–368. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES