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. 1989 Aug 15;262(1):55–61. doi: 10.1042/bj2620055

Heterogeneity of smooth endoplasmic reticulum from rat liver studied by two-phase partitioning.

P Gierow 1, B Jergil 1
PMCID: PMC1133228  PMID: 2554895

Abstract

Smooth microsomal membranes, prepared from rat liver by sucrose-density-gradient centrifugation, were subfractionated by counter-current distribution in an aqueous two-phase system consisting of poly(ethylene glycol) and Dextran T500. A comparison of the distribution curves of marker enzymes, together with theoretically calculated curves, indicated the presence of at least five membrane subfractions, differing in the ratios of the marker enzymes. Glucose-6-phosphatase and arylesterase distributed in one manner, and NADPH-cytochrome c reductase and NADH-ferricyanide reductase in another. Evidence for further heterogeneities in the distribution of marker enzymes in smooth microsomes was obtained by analysing the membrane domain structure using a recently described method [Albertsson (1988) Q. Rev. Biophys. 21, 61-98]. Phenobarbital treatment did not influence the behaviour of the marker enzymes.

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Selected References

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  1. ALBERTSSON P. A. THIN-LAYER COUNTERCURRENT DISTRIBUTION. Anal Biochem. 1965 Apr;11:121–125. doi: 10.1016/0003-2697(65)90050-3. [DOI] [PubMed] [Google Scholar]
  2. Albertsson P. A. Analysis of the domain structure of membranes by fragmentation and separation in aqueous polymer two-phase systems. Q Rev Biophys. 1988 Feb;21(1):61–98. doi: 10.1017/s0033583500005023. [DOI] [PubMed] [Google Scholar]
  3. Amar-Costesec A., Beaufay H. A structural basis of enzymic heterogeneity within liver endoplasmic reticulum. J Theor Biol. 1981 Mar 21;89(2):217–230. doi: 10.1016/0022-5193(81)90309-x. [DOI] [PubMed] [Google Scholar]
  4. Amar-Costesec A., Todd J. A., Kreibich G. Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. I. Functional tests on rat liver microsomal subfractions. J Cell Biol. 1984 Dec;99(6):2247–2253. doi: 10.1083/jcb.99.6.2247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beaufay H., Amar-Costesec A., Thinès-Sempoux D., Wibo M., Robbi M., Berthet J. Analytical study of microsomes and isolated subcellular membranes from rat liver. 3. Subfractionation of the microsomal fraction by isopycnic and differential centrifugation in density gradients. J Cell Biol. 1974 Apr;61(1):213–231. doi: 10.1083/jcb.61.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  7. Depierre J. W., Dallner G. Structural aspects of the membrane of the endoplasmic reticulum. Biochim Biophys Acta. 1975 Dec 29;415(4):411–472. doi: 10.1016/0304-4157(75)90006-4. [DOI] [PubMed] [Google Scholar]
  8. Eriksson L. C., Bergstrand A., Dallner G. Lateral enzyme topology in the rough endoplasmic reticulum of rat liver. Cell Tissue Res. 1977 Mar 30;179(1):17–43. doi: 10.1007/BF00278461. [DOI] [PubMed] [Google Scholar]
  9. FLECK A., MUNRO H. N. The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962 May 14;55:571–583. doi: 10.1016/0006-3002(62)90836-3. [DOI] [PubMed] [Google Scholar]
  10. Gierow P., Jergil B. A spectrophotometric method for the determination of glucose-6-phosphatase activity. Anal Biochem. 1980 Jan 15;101(2):305–309. doi: 10.1016/0003-2697(80)90191-8. [DOI] [PubMed] [Google Scholar]
  11. Gierow P., Jergil B., Albertsson P. A. Lateral heterogeneity of rat liver plasma membranes analysed by counter-current distribution. Biochem J. 1988 Jan 15;249(2):369–375. doi: 10.1042/bj2490369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gierow P., Sommarin M., Larsson C., Jergil B. Fractionation of rat liver plasma-membrane regions by two-phase partitioning. Biochem J. 1986 May 1;235(3):685–691. doi: 10.1042/bj2350685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Glaumann H., Dallner G. Subfractionation of smooth microsomes from rat liver. J Cell Biol. 1970 Oct;47(1):34–48. doi: 10.1083/jcb.47.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hrycay E. G., O'Brien P. J. Microsomal electron transport. II. Reduced nicotinamide adenine dinucleotide--cytochrome b5 reductase and cytochrome P-450 as electron carriers in microsomal NADH-peroxidase activity. Arch Biochem Biophys. 1974 Jan;160(1):230–245. doi: 10.1016/s0003-9861(74)80030-5. [DOI] [PubMed] [Google Scholar]
  15. Lewis J. A., Tata J. R. A rapidly sedimenting fraction of rat liver endoplasmic reticulum. J Cell Sci. 1973 Sep;13(2):447–459. doi: 10.1242/jcs.13.2.447. [DOI] [PubMed] [Google Scholar]
  16. Lewis J. A., Tata J. R. Heterogeneous distribution of glucose 6-phosphatase in rat liver microsomal fractions as shown by adaptation of a cytochemical technique. Biochem J. 1973 May;134(1):69–78. doi: 10.1042/bj1340069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. López-Pérez M. J., París G., Larsson C. Highly purified mitochondria from rat brain prepared by phase partition. Biochim Biophys Acta. 1981 Apr 13;635(2):359–368. doi: 10.1016/0005-2728(81)90034-7. [DOI] [PubMed] [Google Scholar]
  18. Ohlsson R., Jergil B. On the involvement of cytochrome P-450 in the binding of ribosomes to a subfraction of rat-liver rapidly sedimenting endoplasmic reticulum. Eur J Biochem. 1977 Feb;72(3):595–603. doi: 10.1111/j.1432-1033.1977.tb11282.x. [DOI] [PubMed] [Google Scholar]
  19. Ohlsson R., Jergil B., Walter H. Fractionation of microsomal membranes on the basis of their surface properties. Biochem J. 1978 Apr 15;172(1):189–192. doi: 10.1042/bj1720189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PALADE G. E., SIEKEVITZ P. Pancreatic microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Nov 25;2(6):671–690. doi: 10.1083/jcb.2.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shephard E. H., Hübscher G. Phosphatidate biosynthesis in mitochondrial subfractions of rat liver. Biochem J. 1969 Jun;113(2):429–440. doi: 10.1042/bj1130429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Svensson H., Dallner G., Ernster L. Investigation of specificity in membrane breakage occurring during sonication of rough microsomal membranes. Biochim Biophys Acta. 1972 Aug 9;274(2):447–461. doi: 10.1016/0005-2736(72)90190-3. [DOI] [PubMed] [Google Scholar]

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