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. 1989 Aug 15;262(1):353–356. doi: 10.1042/bj2620353

cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.

H Verboomen 1, F Wuytack 1, J A Eggermont 1, S De Jaegere 1, L Missiaen 1, L Raeymaekers 1, R Casteels 1
PMCID: PMC1133268  PMID: 2530978

Abstract

Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino acid residues (Mr 6080). This peptide shares 100% sequence identity with dog cardiac-muscle phospholamban. It differs from rabbit cardiac-muscle and slow-twitch skeletal-muscle phospholamban only at position 2, which is a glutamic acid residue in rabbit phospholamban, but an aspartic acid residue in the pig smooth-muscle protein. Northern-blot analysis reveals the presence of several phospholamban mRNAs in smooth muscle, but a 900-nucleotide-residue and a 2800-nucleotide-residue transcript predominate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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