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. 1989 Sep 15;262(3):849–854. doi: 10.1042/bj2620849

Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase of Actinomadura R39.

C Piron-Fraipont 1, C Duez 1, A Matagne 1, C Molitor 1, J Dusart 1, J M Frère 1, J M Ghuysen 1
PMCID: PMC1133351  PMID: 2590171

Abstract

By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone in S. lividans TK24, the gene that encodes the Actinomadura R39 beta-lactamase. Gene cloning resulted in an amplified expression of the beta-lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg.litre of culture-1).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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