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. 1989 Sep 15;262(3):977–979. doi: 10.1042/bj2620977

4-Hydroxynonenal induces a DNA-binding protein similar to the heat-shock factor.

F Cajone 1, M Salina 1, A Benelli-Zazzera 1
PMCID: PMC1133369  PMID: 2590181

Abstract

By using a gel mobility assay, we have shown that treatment of HeLa cells with 4-hydroxynonenal, a major product of the peroxidation of membrane lipids and an inducer of heat-shock proteins, has the same effect as heat shock in causing the appearance of a protein which binds to the sequence of DNA specific for the induction of heat-shock genes. Lipoperoxidation and heat exposure seem to share a common mechanism of specific gene activation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bochner B. R., Lee P. C., Wilson S. W., Cutler C. W., Ames B. N. AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell. 1984 May;37(1):225–232. doi: 10.1016/0092-8674(84)90318-0. [DOI] [PubMed] [Google Scholar]
  2. Cairo G., Bardella L., Schiaffonati L., Bernelli-Zazzera A. Synthesis of heat shock proteins in rat liver after ischemia and hyperthermia. Hepatology. 1985 May-Jun;5(3):357–361. doi: 10.1002/hep.1840050303. [DOI] [PubMed] [Google Scholar]
  3. Cajone F., Bernelli-Zazzera A. Oxidative stress induces a subset of heat shock proteins in rat hepatocytes and MH1C1 cells. Chem Biol Interact. 1988;65(3):235–246. doi: 10.1016/0009-2797(88)90109-3. [DOI] [PubMed] [Google Scholar]
  4. Courgeon A. M., Rollet E., Becker J., Maisonhaute C., Best-Belpomme M. Hydrogen peroxide (H2O2) induces actin and some heat-shock proteins in Drosophila cells. Eur J Biochem. 1988 Jan 15;171(1-2):163–170. doi: 10.1111/j.1432-1033.1988.tb13772.x. [DOI] [PubMed] [Google Scholar]
  5. Kingston R. E., Schuetz T. J., Larin Z. Heat-inducible human factor that binds to a human hsp70 promoter. Mol Cell Biol. 1987 Apr;7(4):1530–1534. doi: 10.1128/mcb.7.4.1530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lee P. C., Bochner B. R., Ames B. N. AppppA, heat-shock stress, and cell oxidation. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7496–7500. doi: 10.1073/pnas.80.24.7496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Levinson W., Oppermann H., Jackson J. Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells. Biochim Biophys Acta. 1980;606(1):170–180. doi: 10.1016/0005-2787(80)90108-2. [DOI] [PubMed] [Google Scholar]
  8. Mosser D. D., Theodorakis N. G., Morimoto R. I. Coordinate changes in heat shock element-binding activity and HSP70 gene transcription rates in human cells. Mol Cell Biol. 1988 Nov;8(11):4736–4744. doi: 10.1128/mcb.8.11.4736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Munro S., Pelham H. What turns on heat shock genes? Nature. 1985 Oct 10;317(6037):477–478. doi: 10.1038/317477a0. [DOI] [PubMed] [Google Scholar]
  10. Ropp M., Courgeon A. M., Calvayrac R., Best-Belpomme M. The possible role of the superoxide ion in the induction of heat-shock and specific proteins in aerobic Drosophila cells during return to normoxia after a period of anaerobiosis. Can J Biochem Cell Biol. 1983 Jun;61(6):456–461. doi: 10.1139/o83-061. [DOI] [PubMed] [Google Scholar]
  11. Sciandra J. J., Subjeck J. R., Hughes C. S. Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenation. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4843–4847. doi: 10.1073/pnas.81.15.4843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sorger P. K., Lewis M. J., Pelham H. R. Heat shock factor is regulated differently in yeast and HeLa cells. Nature. 1987 Sep 3;329(6134):81–84. doi: 10.1038/329081a0. [DOI] [PubMed] [Google Scholar]
  13. Sorger P. K., Pelham H. R. Purification and characterization of a heat-shock element binding protein from yeast. EMBO J. 1987 Oct;6(10):3035–3041. doi: 10.1002/j.1460-2075.1987.tb02609.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wu C., Wilson S., Walker B., Dawid I., Paisley T., Zimarino V., Ueda H. Purification and properties of Drosophila heat shock activator protein. Science. 1987 Nov 27;238(4831):1247–1253. doi: 10.1126/science.3685975. [DOI] [PubMed] [Google Scholar]
  15. Zimarino V., Wu C. Induction of sequence-specific binding of Drosophila heat shock activator protein without protein synthesis. 1987 Jun 25-Jul 1Nature. 327(6124):727–730. doi: 10.1038/327727a0. [DOI] [PubMed] [Google Scholar]

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