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. 1989 Oct 15;263(2):621–624. doi: 10.1042/bj2630621

Evidence for tight metabolic control of the receptor-activated polyphosphoinositide cycle in human platelets.

V M Steen 1, O B Tysnes 1, H Holmsen 1
PMCID: PMC1133474  PMID: 2557003

Abstract

The [32P]PIP2/[32P]PA and the [32P]PIP/[32P]PA relationships were demonstrated to be remarkably similar after stimulation of [32P]Pi-prelabelled platelets for 90 s with various combinations and concentrations of agonists and inhibitors. Thus the activity of the PI and PIP kinases with the corresponding phosphomonoesterases may be tightly controlled during receptor-mediated platelet stimulation involving phospholipase C activation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdel-Latif A. A. Calcium-mobilizing receptors, polyphosphoinositides, and the generation of second messengers. Pharmacol Rev. 1986 Sep;38(3):227–272. [PubMed] [Google Scholar]
  2. Chahwala S. B., Fleischman L. F., Cantley L. Kinetic analysis of guanosine 5'-O-(3-thiotriphosphate) effects on phosphatidylinositol turnover in NRK cell homogenates. Biochemistry. 1987 Jan 27;26(2):612–622. doi: 10.1021/bi00376a037. [DOI] [PubMed] [Google Scholar]
  3. Crouch M. F., Lapetina E. G. A role for Gi in control of thrombin receptor-phospholipase C coupling in human platelets. J Biol Chem. 1988 Mar 5;263(7):3363–3371. [PubMed] [Google Scholar]
  4. Detwiler T. C., Feinman R. D. Kinetics of the thrombin-induced release of calcium (II) by platelets. Biochemistry. 1973 Jan 16;12(2):282–289. doi: 10.1021/bi00726a017. [DOI] [PubMed] [Google Scholar]
  5. Huang E. M., Detwiler T. C. Thrombin-induced phosphoinositide hydrolysis in platelets. Receptor occupancy and desensitization. Biochem J. 1987 Feb 15;242(1):11–18. doi: 10.1042/bj2420011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Irvine R. F., Moor R. M., Pollock W. K., Smith P. M., Wreggett K. A. Inositol phosphates: proliferation, metabolism and function. Philos Trans R Soc Lond B Biol Sci. 1988 Jul 26;320(1199):281–298. doi: 10.1098/rstb.1988.0077. [DOI] [PubMed] [Google Scholar]
  7. Lundberg G. A., Jergil B., Sundler R. Phosphatidylinositol-4-phosphate kinase from rat brain. Activation by polyamines and inhibition by phosphatidylinositol 4,5-bisphosphate. Eur J Biochem. 1986 Dec 1;161(2):257–262. doi: 10.1111/j.1432-1033.1986.tb10441.x. [DOI] [PubMed] [Google Scholar]
  8. Madshus I. H. Regulation of intracellular pH in eukaryotic cells. Biochem J. 1988 Feb 15;250(1):1–8. doi: 10.1042/bj2500001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rittenhouse S. E. Activation of human platelet phospholipase C by ionophore A23187 is totally dependent upon cyclo-oxygenase products and ADP. Biochem J. 1984 Aug 15;222(1):103–110. doi: 10.1042/bj2220103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rittenhouse S. E., Banga H. S., Sasson J. P., King W. G., Tarver A. P. Regulation of platelet phospholipase C. Philos Trans R Soc Lond B Biol Sci. 1988 Jul 26;320(1199):299–311. doi: 10.1098/rstb.1988.0078. [DOI] [PubMed] [Google Scholar]
  11. Shukla S. D., Franklin C. C., Carter M. G. Activation of phospholipase C in platelets by platelet activating factor and thrombin causes hydrolysis of a common pool of phosphatidylinositol 4,5-bisphosphate. Biochim Biophys Acta. 1987 Jul 6;929(2):134–141. doi: 10.1016/0167-4889(87)90168-6. [DOI] [PubMed] [Google Scholar]
  12. Siffert W., Akkerman J. W. Na+/H+ exchange as a modulator of platelet activation. Trends Biochem Sci. 1988 Apr;13(4):148–151. doi: 10.1016/0968-0004(88)90074-6. [DOI] [PubMed] [Google Scholar]
  13. Steen V. M., Holmsen H. Synergism between thrombin and epinephrine in human platelets: different dose-response relationships for aggregation and dense granule secretion. Thromb Haemost. 1985 Oct 30;54(3):680–683. [PubMed] [Google Scholar]
  14. Steen V. M., Tysnes O. B., Holmsen H. Synergism between thrombin and adrenaline (epinephrine) in human platelets. Marked potentiation of inositol phospholipid metabolism. Biochem J. 1988 Jul 15;253(2):581–586. doi: 10.1042/bj2530581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tam S. W., Fenton J. W., 2nd, Detwiler T. C. Dissociation of thrombin from platelets by hirudin. Evidence for receptor processing. J Biol Chem. 1979 Sep 25;254(18):8723–8725. [PubMed] [Google Scholar]
  16. Tysnes O. B., Steen V. M., Holmsen H. Neomycin inhibits platelet functions and inositol phospholipid metabolism upon stimulation with thrombin, but not with ionomycin or 12-O-tetradecanoyl-phorbol 13-acetate. Eur J Biochem. 1988 Oct 15;177(1):219–223. doi: 10.1111/j.1432-1033.1988.tb14365.x. [DOI] [PubMed] [Google Scholar]
  17. Tysnes O. B., Verhoeven A. J., Aarbakke G. M., Holmsen H. Phosphoinositide metabolism in resting and thrombin-stimulated human platelets. Evidence against metabolic heterogeneity. FEBS Lett. 1987 Jun 22;218(1):68–72. doi: 10.1016/0014-5793(87)81020-7. [DOI] [PubMed] [Google Scholar]
  18. Tysnes O. B., Verhoeven A. J., Holmsen H. Neomycin inhibits agonist-stimulated polyphosphoinositide metabolism and responses in human platelets. Biochem Biophys Res Commun. 1987 Apr 14;144(1):454–462. doi: 10.1016/s0006-291x(87)80531-4. [DOI] [PubMed] [Google Scholar]
  19. Van Rooijen L. A., Rossowska M., Bazan N. G. Inhibition of phosphatidylinositol-4-phosphate kinase by its product phosphatidylinositol-4,5-bisphosphate. Biochem Biophys Res Commun. 1985 Jan 16;126(1):150–155. doi: 10.1016/0006-291x(85)90584-4. [DOI] [PubMed] [Google Scholar]
  20. Verhoeven A. J., Tysnes O. B., Aarbakke G. M., Cook C. A., Holmsen H. Turnover of the phosphomonoester groups of polyphosphoinositol lipids in unstimulated human platelets. Eur J Biochem. 1987 Jul 1;166(1):3–9. doi: 10.1111/j.1432-1033.1987.tb13475.x. [DOI] [PubMed] [Google Scholar]
  21. de Chaffoy de Courcelles D., Roevens P., Van Belle H., De Clerck F. The synergistic effect of serotonin and epinephrine on the human platelet at the level of signal transduction. FEBS Lett. 1987 Jul 27;219(2):283–288. doi: 10.1016/0014-5793(87)80236-3. [DOI] [PubMed] [Google Scholar]

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