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. 1989 Nov 1;263(3):703–708. doi: 10.1042/bj2630703

Turnover of carnitine palmitoyltransferase mRNA and protein in H4IIE cells. Effect of cyclic AMP and insulin.

L Wang 1, P S Brady 1, L J Brady 1
PMCID: PMC1133489  PMID: 2557007

Abstract

Regulation of the 68 kDa carnitine palmitoyltransferase (CPT) synthesis by (chlorophenylthio) cyclic AMP (cAMP) and insulin was studied in H4IIE cells in culture. Addition of 0.1 mM- or 1.0 mM-(chlorophenylthio) cAMP induced CPT mRNA and rate of transcription 2-4-fold by 15 min, reaching a plateau at 4-6-fold by 30 min. Addition of 5-15 nM-insulin plus 1.0 mM-cAMP suppressed the increases in transcription rate and mRNA levels occurring with cAMP alone. The t1/2 for CPT mRNA was 70-80 min and was not affected by cAMP. The t1/2 for CPT protein was 70 min, and was increased to 240 min in the presence of cAMP. The rate of CPT synthesis was also increased in the presence of cAMP. The data indicate that CPT synthesis is increased by cAMP via induction of transcription and subsequent increase in the CPT mRNA. Insulin acts to depress transcription and CPT mRNA. In addition, cAMP prolongs the t1/2 of CPT.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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