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. 1989 Nov 15;264(1):133–136. doi: 10.1042/bj2640133

Kallikrein-gene expression in the rat gastrointestinal tract.

P J Fuller 1, K Verity 1, B A Matheson 1, J A Clements 1
PMCID: PMC1133556  PMID: 2604709

Abstract

The serine proteinase glandular kallikrein has been demonstrated in the gastrointestinal tract, although there is some doubt as to whether it is synthesized there or derives from exocrine-gland secretions. Using a rat pancreatic kallikrein cRNA probe we have demonstrated kallikrein-like gene expression in the corpus, duodenum, jejunum, ileum, caecum and colon, and compared the pattern of expression with that of the gastrointestinal peptides somatostatin, gastrin and glucagon. In addition, using a panel of oligonucleotide probes specific for various members of the rat kallikrein-gene family, we have shown that the kallikrein-like gene expressed appears to be expressed as true kallikrein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashley P. L., MacDonald R. J. Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin. Biochemistry. 1985 Aug 13;24(17):4512–4520. doi: 10.1021/bi00338a005. [DOI] [PubMed] [Google Scholar]
  2. Ashley P. L., MacDonald R. J. Tissue-specific expression of kallikrein-related genes in the rat. Biochemistry. 1985 Aug 13;24(17):4520–4527. doi: 10.1021/bi00338a006. [DOI] [PubMed] [Google Scholar]
  3. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baker A. R., Shine J. Human kidney kallikrein: cDNA cloning and sequence analysis. DNA. 1985 Dec;4(6):445–450. doi: 10.1089/dna.1985.4.445. [DOI] [PubMed] [Google Scholar]
  5. Brand S. J., Fuller P. J. Differential gastrin gene expression in rat gastrointestinal tract and pancreas during neonatal development. J Biol Chem. 1988 Apr 15;263(11):5341–5347. [PubMed] [Google Scholar]
  6. Chen Y. P., Chao J., Chao L. Molecular cloning and characterization of two rat renal kallikrein genes. Biochemistry. 1988 Sep 20;27(19):7189–7196. doi: 10.1021/bi00419a005. [DOI] [PubMed] [Google Scholar]
  7. Clements J. A., Fuller P. J., McNally M., Nikolaidis I., Funder J. W. Estrogen regulation of kallikrein gene expression in the rat anterior pituitary. Endocrinology. 1986 Jul;119(1):268–273. doi: 10.1210/endo-119-1-268. [DOI] [PubMed] [Google Scholar]
  8. Clements J. A., Matheson B. A., Wines D. R., Brady J. M., MacDonald R. J., Funder J. W. Androgen dependence of specific kallikrein gene family members expressed in rat prostate. J Biol Chem. 1988 Nov 5;263(31):16132–16137. [PubMed] [Google Scholar]
  9. Currie M. G., Geller D. M., Chao J., Margolius H. S., Needleman P. Kallikrein activation of a high molecular weight atrial peptide. Biochem Biophys Res Commun. 1984 Apr 30;120(2):461–466. doi: 10.1016/0006-291x(84)91276-2. [DOI] [PubMed] [Google Scholar]
  10. Cuthbert A. W., MacVinish L. J. Transporting epithelia as targets for kinin effects. Adv Exp Med Biol. 1986;198(Pt A):203–210. doi: 10.1007/978-1-4684-5143-6_28. [DOI] [PubMed] [Google Scholar]
  11. Evans B. A., Drinkwater C. C., Richards R. I. Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus. J Biol Chem. 1987 Jun 15;262(17):8027–8034. [PubMed] [Google Scholar]
  12. Fuller P. J., Clements J. A., Nikolaidis I., Hiwatari M., Funder J. W. Expression of the renal kallikrein gene in mineralocorticoid-treated and genetically hypertensive rats. J Hypertens. 1986 Aug;4(4):427–433. doi: 10.1097/00004872-198608000-00006. [DOI] [PubMed] [Google Scholar]
  13. Fuller P. J., Funder J. W. The cellular physiology of glandular kallikrein. Kidney Int. 1986 May;29(5):953–964. doi: 10.1038/ki.1986.93. [DOI] [PubMed] [Google Scholar]
  14. Fuller P. J., Stone D. L., Brand S. J. Molecular cloning and sequencing of a rat preprogastrin complementary deoxyribonucleic acid. Mol Endocrinol. 1987 Apr;1(4):306–311. doi: 10.1210/mend-1-4-306. [DOI] [PubMed] [Google Scholar]
  15. Gerald W. L., Chao J., Chao L. Immunological identification of rat tissue kallikrein cDNA and characterization of the kallikrein gene family. Biochim Biophys Acta. 1986 Feb 24;866(1):1–14. doi: 10.1016/0167-4781(86)90093-x. [DOI] [PubMed] [Google Scholar]
  16. Isackson P. J., Dunbar J. C., Bradshaw R. A. Role of glandular kallikreins as growth factor processing enzymes: structural and evolutionary considerations. J Cell Biochem. 1987 Jan;33(1):65–75. doi: 10.1002/jcb.240330107. [DOI] [PubMed] [Google Scholar]
  17. Kobayashi M., Ohata K. Comparison of kinins derived from rat stomach tissue and produced by catheptic enzyme in rat stomach. Jpn J Pharmacol. 1984 Oct;36(2):169–175. doi: 10.1254/jjp.36.169. [DOI] [PubMed] [Google Scholar]
  18. Lemischka I. R., Farmer S., Racaniello V. R., Sharp P. A. Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA. J Mol Biol. 1981 Sep 5;151(1):101–120. doi: 10.1016/0022-2836(81)90223-0. [DOI] [PubMed] [Google Scholar]
  19. MacDonald R. J., Margolius H. S., Erdös E. G. Molecular biology of tissue kallikrein. Biochem J. 1988 Jul 15;253(2):313–321. doi: 10.1042/bj2530313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miller D. H., Chao J., Margolius H. S. Tissue kallikrein synthesis and its modification by testosterone or low dietary sodium. Biochem J. 1984 Feb 15;218(1):37–43. doi: 10.1042/bj2180037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Musch M. W., Kachur J. F., Miller R. J., Field M., Stoff J. S. Bradykinin-stimulated electrolyte secretion in rabbit and guinea pig intestine. Involvement of arachidonic acid metabolites. J Clin Invest. 1983 May;71(5):1073–1083. doi: 10.1172/JCI110857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Overlack A., Scicli A. G., Carretero O. A. Intestinal absorption of glandular kallikrein in the rat. Am J Physiol. 1983 Jun;244(6):G689–G694. doi: 10.1152/ajpgi.1983.244.6.G689. [DOI] [PubMed] [Google Scholar]
  24. Pritchett D. B., Roberts J. L. Dopamine regulates expression of the glandular-type kallikrein gene at the transcriptional level in the pituitary. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5545–5549. doi: 10.1073/pnas.84.16.5545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schachter M. Kallikreins (kininogenases)--a group of serine proteases with bioregulatory actions. Pharmacol Rev. 1979 Mar;31(1):1–17. [PubMed] [Google Scholar]
  26. Schachter M., Longridge D. J., Wheeler G. D., Mehta J. G., Uchida Y. Immunocytochemical and enzyme histochemical localization of kallikrein-like enzymes in colon, intestine, and stomach of rat and cat. J Histochem Cytochem. 1986 Jul;34(7):927–934. doi: 10.1177/34.7.3519756. [DOI] [PubMed] [Google Scholar]
  27. Schachter M., Peret M. W., Billing A. G., Wheeler G. D. Immunolocalization of the protease kallikrein in the colon. J Histochem Cytochem. 1983 Nov;31(11):1255–1260. doi: 10.1177/31.11.6194202. [DOI] [PubMed] [Google Scholar]
  28. Sealey J. E., Atlas S. A., Laragh J. H., Oza N. B., Ryan J. W. Human urinary kallikrein converts inactive to active renin and is a possible physiological activator of renin. Nature. 1978 Sep 14;275(5676):144–145. doi: 10.1038/275144a0. [DOI] [PubMed] [Google Scholar]
  29. Seidah N. G., Chan J. S., Mardini G., Benjannet S., Chrétien M., Boucher R., Genest J. Specific cleavage of beta-LPH and ACTH by tonin: release of an opiate-like peptide beta-LPH (61-78). Biochem Biophys Res Commun. 1979 Feb 28;86(4):1002–1013. doi: 10.1016/0006-291x(79)90217-1. [DOI] [PubMed] [Google Scholar]
  30. Seki T., Nakajima T., Erdös E. G. Colon kallikrein, its relation to the plasma enzyme. Biochem Pharmacol. 1972 May 1;21(9):1227–1235. doi: 10.1016/0006-2952(72)90284-5. [DOI] [PubMed] [Google Scholar]
  31. Skagen D. W., Andersen K. J. Intestinal kallikrein activity is reduced in a bypassed segment of the small intestine in the rat. Comp Biochem Physiol A Comp Physiol. 1986;84(1):175–180. doi: 10.1016/0300-9629(86)90060-5. [DOI] [PubMed] [Google Scholar]
  32. Swift G. H., Dagorn J. C., Ashley P. L., Cummings S. W., MacDonald R. J. Rat pancreatic kallikrein mRNA: nucleotide sequence and amino acid sequence of the encoded preproenzyme. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7263–7267. doi: 10.1073/pnas.79.23.7263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Thomas P. S. Hybridization of denatured RNA transferred or dotted nitrocellulose paper. Methods Enzymol. 1983;100:255–266. doi: 10.1016/0076-6879(83)00060-9. [DOI] [PubMed] [Google Scholar]
  34. Uchida K., Niinobe M., Kato H., Fujii S. Purification and properties of rat stomach kallikrein. Biochim Biophys Acta. 1980 Aug 7;614(2):501–510. doi: 10.1016/0005-2744(80)90239-9. [DOI] [PubMed] [Google Scholar]
  35. Zeitlin I. J., Al-Dhahir H. A., Cook S., Currie A., Donovan K. Bile acids and the intestinal kallikrein-kinin system. Adv Exp Med Biol. 1986;198(Pt A):47–53. doi: 10.1007/978-1-4684-5143-6_7. [DOI] [PubMed] [Google Scholar]
  36. van Leeuwen B. H., Penschow J. D., Coghlan J. P., Richards R. I. Cellular basis for the differential response of mouse kallikrein genes to hormonal induction. EMBO J. 1987 Jun;6(6):1705–1713. doi: 10.1002/j.1460-2075.1987.tb02421.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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