Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1989 Dec 15;264(3):765–769. doi: 10.1042/bj2640765

Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.

F Wuytack 1, J A Eggermont 1, L Raeymaekers 1, L Plessers 1, R Casteels 1
PMCID: PMC1133651  PMID: 2482734

Abstract

We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2 encoding the sarcoplasmic reticulum Ca2+ pump in slow skeletal and cardiac muscle. This alternatively spliced transcript was shown to be mainly represented in non-muscle tissues. These antibodies have enabled us to show the presence of the unique C-terminus of this type of Ca2+ pump, as predicted from the cDNA sequence, in the endoplasmic reticulum of vascular and gastric smooth muscle, liver and kidney.

Full text

PDF
765

Images in this article

766Fig. 1.
on p.766
767Fig. 2.
on p.767
768Fig. 3.
on p.768

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandl C. J., Green N. M., Korczak B., MacLennan D. H. Two Ca2+ ATPase genes: homologies and mechanistic implications of deduced amino acid sequences. Cell. 1986 Feb 28;44(4):597–607. doi: 10.1016/0092-8674(86)90269-2. [DOI] [PubMed] [Google Scholar]
  2. Brandl C. J., deLeon S., Martin D. R., MacLennan D. H. Adult forms of the Ca2+ATPase of sarcoplasmic reticulum. Expression in developing skeletal muscle. J Biol Chem. 1987 Mar 15;262(8):3768–3774. [PubMed] [Google Scholar]
  3. Chiesi M., Guerini D. Characterization of heart cytosolic proteins capable of modulating calcium uptake by the sarcoplasmic reticulum. 1. Isolation of a protein with protective activity and its identification as muscle albumin. Eur J Biochem. 1987 Jan 15;162(2):365–370. doi: 10.1111/j.1432-1033.1987.tb10610.x. [DOI] [PubMed] [Google Scholar]
  4. De Smedt H., Parys J. B., Borghgraef R., Wuytack F. Phosphorylated intermediates of (Ca2+ + Mg2+)-ATPase and alkaline phosphatase in renal plasma membranes. Biochim Biophys Acta. 1983 Mar 9;728(3):409–418. doi: 10.1016/0005-2736(83)90513-8. [DOI] [PubMed] [Google Scholar]
  5. Eggermont J. A., Wuytack F., De Jaegere S., Nelles L., Casteels R. Evidence for two isoforms of the endoplasmic-reticulum Ca2+ pump in pig smooth muscle. Biochem J. 1989 Jun 15;260(3):757–761. doi: 10.1042/bj2600757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gaetani S., Smith J. A., Feldman R. A., Morimoto T. Preparation of rough microsomes and membrane-bound polysomes that are active in protein synthesis in vitro. Methods Enzymol. 1983;96:3–24. doi: 10.1016/s0076-6879(83)96005-6. [DOI] [PubMed] [Google Scholar]
  7. Gunteski-Hamblin A. M., Greeb J., Shull G. E. A novel Ca2+ pump expressed in brain, kidney, and stomach is encoded by an alternative transcript of the slow-twitch muscle sarcoplasmic reticulum Ca-ATPase gene. Identification of cDNAs encoding Ca2+ and other cation-transporting ATPases using an oligonucleotide probe derived from the ATP-binding site. J Biol Chem. 1988 Oct 15;263(29):15032–15040. [PubMed] [Google Scholar]
  8. Jorgensen A. O., Arnold W., Pepper D. R., Kahl S. D., Mandel F., Campbell K. P. A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study. Cell Motil Cytoskeleton. 1988;9(2):164–174. doi: 10.1002/cm.970090208. [DOI] [PubMed] [Google Scholar]
  9. Komuro I., Kurabayashi M., Shibazaki Y., Takaku F., Yazaki Y. Molecular cloning and characterization of a Ca2+ + Mg2+-dependent adenosine triphosphatase from rat cardiac sarcoplasmic reticulum. Regulation of its expression by pressure overload and developmental stage. J Clin Invest. 1989 Apr;83(4):1102–1108. doi: 10.1172/JCI113989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Korczak B., Zarain-Herzberg A., Brandl C. J., Ingles C. J., Green N. M., MacLennan D. H. Structure of the rabbit fast-twitch skeletal muscle Ca2+-ATPase gene. J Biol Chem. 1988 Apr 5;263(10):4813–4819. [PubMed] [Google Scholar]
  11. Lompre A. M., de la Bastie D., Boheler K. R., Schwartz K. Characterization and expression of the rat heart sarcoplasmic reticulum Ca2+-ATPase mRNA. FEBS Lett. 1989 May 22;249(1):35–41. doi: 10.1016/0014-5793(89)80010-9. [DOI] [PubMed] [Google Scholar]
  12. Lytton J., MacLennan D. H. Molecular cloning of cDNAs from human kidney coding for two alternatively spliced products of the cardiac Ca2+-ATPase gene. J Biol Chem. 1988 Oct 15;263(29):15024–15031. [PubMed] [Google Scholar]
  13. Lytton J., Zarain-Herzberg A., Periasamy M., MacLennan D. H. Molecular cloning of the mammalian smooth muscle sarco(endo)plasmic reticulum Ca2+-ATPase. J Biol Chem. 1989 Apr 25;264(12):7059–7065. [PubMed] [Google Scholar]
  14. MacLennan D. H., Brandl C. J., Korczak B., Green N. M. Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence. Nature. 1985 Aug 22;316(6030):696–700. doi: 10.1038/316696a0. [DOI] [PubMed] [Google Scholar]
  15. Raeymaekers L., Wuytack F., Casteels R. Subcellular fractionation of pig stomach smooth muscle. A study of the distribution of the (Ca2+ + Mg2+)-ATPase activity in plasmalemma and endoplasmic reticulum. Biochim Biophys Acta. 1985 May 28;815(3):441–454. doi: 10.1016/0005-2736(85)90372-4. [DOI] [PubMed] [Google Scholar]
  16. Redinbaugh M. G., Turley R. B. Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions. Anal Biochem. 1986 Mar;153(2):267–271. doi: 10.1016/0003-2697(86)90091-6. [DOI] [PubMed] [Google Scholar]
  17. Wuytack F., Kanmura Y., Eggermont J. A., Raeymaekers L., Verbist J., Hartweg D., Gietzen K., Casteels R. Smooth muscle expresses a cardiac/slow muscle isoform of the Ca2+-transport ATPase in its endoplasmic reticulum. Biochem J. 1989 Jan 1;257(1):117–123. doi: 10.1042/bj2570117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. de la Bastie D., Wisnewsky C., Schwartz K., Lompré A. M. (Ca2+ + Mg2+)-dependent ATPase mRNA from smooth muscle sarcoplasmic reticulum differs from that in cardiac and fast skeletal muscles. FEBS Lett. 1988 Feb 29;229(1):45–48. doi: 10.1016/0014-5793(88)80794-4. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES