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. 1990 Feb 1;265(3):899–902. doi: 10.1042/bj2650899

Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate.

T R Hawkes 1, T Lewis 1, J R Coggins 1, D M Mousdale 1, D J Lowe 1, R N Thorneley 1
PMCID: PMC1133717  PMID: 2407239

Abstract

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coggins J. R., Boocock M. R., Chaudhuri S., Lambert J. M., Lumsden J., Nimmo G. A., Smith D. D. The arom multifunctional enzyme from Neurospora crassa. Methods Enzymol. 1987;142:325–341. doi: 10.1016/s0076-6879(87)42044-2. [DOI] [PubMed] [Google Scholar]
  2. Floss H. G., Onderka D. K., Carroll M. Stereochemistry of the 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase reaction and the chorismate synthetase reaction. J Biol Chem. 1972 Feb 10;247(3):736–744. [PubMed] [Google Scholar]
  3. Hill R. K., Newkome G. R. Stereochemistry of chorismic acid biosynthesis. J Am Chem Soc. 1969 Oct 8;91(21):5893–5894. doi: 10.1021/ja01049a045. [DOI] [PubMed] [Google Scholar]
  4. Lalégerie P., Legler G., Yon J. M. The use of inhibitors in the study of glycosidases. Biochimie. 1982 Nov-Dec;64(11-12):977–1000. doi: 10.1016/s0300-9084(82)80379-9. [DOI] [PubMed] [Google Scholar]
  5. Lanzetta P. A., Alvarez L. J., Reinach P. S., Candia O. A. An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem. 1979 Nov 15;100(1):95–97. doi: 10.1016/0003-2697(79)90115-5. [DOI] [PubMed] [Google Scholar]
  6. Steinrücken H. C., Amrhein N. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase. Biochem Biophys Res Commun. 1980 Jun 30;94(4):1207–1212. doi: 10.1016/0006-291x(80)90547-1. [DOI] [PubMed] [Google Scholar]
  7. White P. J., Millar G., Coggins J. R. The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa. Biochem J. 1988 Apr 15;251(2):313–322. doi: 10.1042/bj2510313. [DOI] [PMC free article] [PubMed] [Google Scholar]

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