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. 1993 Oct 1;295(Pt 1):221–225. doi: 10.1042/bj2950221

Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

M Oñaderra 1, J M Mancheño 1, M Gasset 1, J Lacadena 1, G Schiavo 1, A Martínez del Pozo 1, J G Gavilanes 1
PMCID: PMC1134842  PMID: 8216220

Abstract

alpha-Sarcin is a cytotoxic protein produced by the mould Aspergillus giganteus. Insertion of alpha-sarcin into asolectin membranes has been demonstrated by protein labelling with photoreactive phospholipids. alpha-Sarcin added externally to tRNA-containing asolectin liposomes degrades the entrapped tRNA. Trypsin-containing asolectin liposomes were also prepared. Encapsulated trypsin degrades alpha-sarcin, even in the presence of a large excess of external hen egg-white trypsin inhibitor to prevent any alpha-sarin degradation outside the vesicles. These processes occur only with acidic phospholipids and were not observed when phosphatidylcholine vesicles were used. These results indicate that alpha-sarcin penetrates the lipid bilayer and becomes exposed to the lumen of negatively charged liposomes.

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Selected References

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