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. 1993 Oct 15;295(Pt 2):347–350. doi: 10.1042/bj2950347

Molecular cloning and deduced amino acid sequences of the gamma-subunits of rat and monkey NAD(+)-isocitrate dehydrogenases.

B J Nichols 1, L Hall 1, A C Perry 1, R M Denton 1
PMCID: PMC1134888  PMID: 8240232

Abstract

A 600 bp cDNA fragment encoding part of the gamma-subunit of pig heart NAD(+)-isocitrate dehydrogenase (ICDH gamma) was amplified by PCR using redundant oligonucleotide primers based on partial peptide sequence data [Huang and Colman (1990) Biochemistry 29, 8266-8273]. This PCR fragment was then used as a probe to isolate clones encoding the complete mature forms of the gamma-subunit from rat epididymis and monkey testis cDNA libraries. Comparison of the deduced amino acid sequences of the rat and monkey subunits and the partial sequence of the pig heart enzyme revealed a remarkably high level of sequence identity. The relationship between the deduced amino acid sequences of the NAD(+)-ICDH gamma-subunits and those of nonmammalian NAD(+)- and NADP(+)-ICDH subunits is discussed.

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Selected References

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  1. Bednar R. A., Colman R. F. Chemical modification A probe of the structure and function of the subunits of DPN-dependent isocitrate dehydrogenase. J Biol Chem. 1982 Oct 10;257(19):11734–11739. [PubMed] [Google Scholar]
  2. Colman R. F. Mechanisms for the oxidative decarboxylation of isocitrate: implications for control. Adv Enzyme Regul. 1975;13:413–433. doi: 10.1016/0065-2571(75)90028-x. [DOI] [PubMed] [Google Scholar]
  3. Cupp J. R., McAlister-Henn L. Cloning and characterization of the gene encoding the IDH1 subunit of NAD(+)-dependent isocitrate dehydrogenase from Saccharomyces cerevisiae. J Biol Chem. 1992 Aug 15;267(23):16417–16423. [PubMed] [Google Scholar]
  4. Cupp J. R., McAlister-Henn L. NAD(+)-dependent isocitrate dehydrogenase. Cloning, nucleotide sequence, and disruption of the IDH2 gene from Saccharomyces cerevisiae. J Biol Chem. 1991 Nov 25;266(33):22199–22205. [PubMed] [Google Scholar]
  5. Denton R. M., McCormack J. G. Ca2+ as a second messenger within mitochondria of the heart and other tissues. Annu Rev Physiol. 1990;52:451–466. doi: 10.1146/annurev.ph.52.030190.002315. [DOI] [PubMed] [Google Scholar]
  6. Denton R. M., Richards D. A., Chin J. G. Calcium ions and the regulation of NAD+-linked isocitrate dehydrogenase from the mitochondria of rat heart and other tissues. Biochem J. 1978 Dec 15;176(3):899–906. doi: 10.1042/bj1760899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ehrlich R. S., Hayman S., Ramachandran N., Colman R. F. Re-evaluation of molecular weight of pig heart NAD-specific isocitrate dehydrogenase. J Biol Chem. 1981 Oct 25;256(20):10560–10564. [PubMed] [Google Scholar]
  8. Gabriel J. L., Milner R., Plaut G. W. Inhibition and activation of bovine heart NAD-specific isocitrate dehydrogenase by ATP. Arch Biochem Biophys. 1985 Jul;240(1):128–134. doi: 10.1016/0003-9861(85)90015-3. [DOI] [PubMed] [Google Scholar]
  9. Hartl F. U., Neupert W. Protein sorting to mitochondria: evolutionary conservations of folding and assembly. Science. 1990 Feb 23;247(4945):930–938. doi: 10.1126/science.2406905. [DOI] [PubMed] [Google Scholar]
  10. Haselbeck R. J., McAlister-Henn L. Function and expression of yeast mitochondrial NAD- and NADP-specific isocitrate dehydrogenases. J Biol Chem. 1993 Jun 5;268(16):12116–12122. [PubMed] [Google Scholar]
  11. Huang Y. C., Colman R. F. Subunit location and sequences of the cysteinyl peptides of pig heart NAD-dependent isocitrate dehydrogenase. Biochemistry. 1990 Sep 11;29(36):8266–8273. doi: 10.1021/bi00488a010. [DOI] [PubMed] [Google Scholar]
  12. Hurley J. H., Dean A. M., Koshland D. E., Jr, Stroud R. M. Catalytic mechanism of NADP(+)-dependent isocitrate dehydrogenase: implications from the structures of magnesium-isocitrate and NADP+ complexes. Biochemistry. 1991 Sep 3;30(35):8671–8678. doi: 10.1021/bi00099a026. [DOI] [PubMed] [Google Scholar]
  13. Imada K., Sato M., Tanaka N., Katsube Y., Matsuura Y., Oshima T. Three-dimensional structure of a highly thermostable enzyme, 3-isopropylmalate dehydrogenase of Thermus thermophilus at 2.2 A resolution. J Mol Biol. 1991 Dec 5;222(3):725–738. doi: 10.1016/0022-2836(91)90508-4. [DOI] [PubMed] [Google Scholar]
  14. McCormack J. G., Denton R. M. A comparative study of the regulation of Ca2+ of the activities of the 2-oxoglutarate dehydrogenase complex and NAD+-isocitrate dehydrogenase from a variety of sources. Biochem J. 1981 May 15;196(2):619–624. doi: 10.1042/bj1960619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McCormack J. G., Halestrap A. P., Denton R. M. Role of calcium ions in regulation of mammalian intramitochondrial metabolism. Physiol Rev. 1990 Apr;70(2):391–425. doi: 10.1152/physrev.1990.70.2.391. [DOI] [PubMed] [Google Scholar]
  16. Perry A. C., Jones R., Barker P. J., Hall L. A mammalian epididymal protein with remarkable sequence similarity to snake venom haemorrhagic peptides. Biochem J. 1992 Sep 15;286(Pt 3):671–675. doi: 10.1042/bj2860671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perry A. C., Jones R., Niang L. S., Jackson R. M., Hall L. Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon. Biochem J. 1992 Aug 1;285(Pt 3):863–870. doi: 10.1042/bj2850863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ramachandran N., Colman R. F. Chemical characterization of distinct subunits of pig heart DPN-specific isocitrate dehydrogenase. J Biol Chem. 1980 Sep 25;255(18):8859–8864. [PubMed] [Google Scholar]
  19. Rutter G. A., Denton R. M. Rapid purification of pig heart NAD+-isocitrate dehydrogenase. Studies on the regulation of activity by Ca2+, adenine nucleotides, Mg2+ and other metal ions. Biochem J. 1989 Oct 15;263(2):445–452. doi: 10.1042/bj2630445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rutter G. A., Denton R. M. The binding of Ca2+ ions to pig heart NAD+-isocitrate dehydrogenase and the 2-oxoglutarate dehydrogenase complex. Biochem J. 1989 Oct 15;263(2):453–462. doi: 10.1042/bj2630453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Saha A., Huang Y. C., Colman R. F. Cysteinyl peptide labeled by 3-bromo-2-ketoglutarate in the active site of pig heart NAD+-dependent isocitrate dehydrogenase. Biochemistry. 1989 Oct 17;28(21):8425–8431. doi: 10.1021/bi00447a023. [DOI] [PubMed] [Google Scholar]
  22. Thorsness P. E., Koshland D. E., Jr Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate. J Biol Chem. 1987 Aug 5;262(22):10422–10425. [PubMed] [Google Scholar]
  23. Walker J. E., Saraste M., Runswick M. J., Gay N. J. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982;1(8):945–951. doi: 10.1002/j.1460-2075.1982.tb01276.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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