Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1988 Sep 1;254(2):367–372. doi: 10.1042/bj2540367

Ornithine decarboxylase antizyme in kidneys of male and female mice.

Y Murakami 1, M Marumo 1, S Hayashi 1
PMCID: PMC1135086  PMID: 3178765

Abstract

Antizyme, a protein inhibitor of ornithine decarboxylase (ODC), was shown to be induced in mouse kidney by repeated injection of putrescine. Antizyme was also present as a complex with ODC in the kidney of untreated mouse. The amount of the renal ODC-antizyme complex was 3-fold higher in male mice than in female mice. On the contrary, the proportion of ODC present as a complex with antizyme was 24-fold higher in females than in males, and the decay of renal ODC activity after cycloheximide treatment was about 5-fold more rapid in females than in males. Administration of testosterone to female mice, a procedure known to prolong the half-life of renal ODC, increased both ODC activity and the content of ODC-antizyme complex, but decreased the antizyme/ODC ratio in the kidney. These results are consistent with the previous observation in HTC cells that the decay rate of ODC activity in the presence of cycloheximide correlated well with the proportion of ODC present as a complex with antizyme, suggesting the ubiquitous role of antizyme in ODC degradation.

Full text

PDF
367

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brosnan M. E., Farrell R., Wilansky H., Williamson D. H. Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats. Biochem J. 1983 Apr 15;212(1):149–153. doi: 10.1042/bj2120149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Canellakis E. S., Viceps-Madore D., Kyriakidis D. A., Heller J. S. The regulation and function of ornithine decarboxylase and of the polyamines. Curr Top Cell Regul. 1979;15:155–202. [PubMed] [Google Scholar]
  3. Flamigni F., Stefanelli C., Guarnieri C., Caldarera C. M. Modulation of ornithine decarboxylase activity and ornithine decarboxylase-antizyme complex in rat heart by hormone and putrescine treatment. Biochim Biophys Acta. 1986 Jul 16;882(3):377–383. doi: 10.1016/0304-4165(86)90261-8. [DOI] [PubMed] [Google Scholar]
  4. Fong W. F., Heller J. S., Canellakis E. S. The appearance of an ornithine decarboxylase inhibitory protein upon the addition of putrescine to cell cultures. Biochim Biophys Acta. 1976 Apr 23;428(2):456–465. doi: 10.1016/0304-4165(76)90054-4. [DOI] [PubMed] [Google Scholar]
  5. Fujita K., Matsufuji S., Murakami Y., Hayashi S. Antizyme to ornithine decarboxylase is present in the liver of starved rats. Biochem J. 1984 Mar 1;218(2):557–562. doi: 10.1042/bj2180557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fujita K., Murakami Y., Hayashi S. A macromolecular inhibitor of the antizyme to ornithine decarboxylase. Biochem J. 1982 Jun 15;204(3):647–652. doi: 10.1042/bj2040647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grahn B., Henningsson S. S., Kahlson G., Rosengren E. Alterations in the activities of ornithine and histidine decarboxylases provoked by testosterone in mice. Br J Pharmacol. 1973 May;48(1):113–120. doi: 10.1111/j.1476-5381.1973.tb08228.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayashi S., Fujita K. Antizyme and antizyme inhibitor of ornithine decarboxylase (rat liver). Methods Enzymol. 1983;94:185–193. doi: 10.1016/s0076-6879(83)94031-4. [DOI] [PubMed] [Google Scholar]
  9. Hayashi S., Kameji T., Fujita K., Murakami Y., Kanamoto R., Utsunomiya K., Matsufuji S., Takiguchi M., Mori M., Tatibana M. Molecular mechanism for the regulation of hepatic ornithine decarboxylase. Adv Enzyme Regul. 1985;23:311–329. doi: 10.1016/0065-2571(85)90054-8. [DOI] [PubMed] [Google Scholar]
  10. Heller J. S., Fong W. F., Canellakis E. S. Induction of a protein inhibitor to ornithine decarboxylase by the end products of its reaction. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1858–1862. doi: 10.1073/pnas.73.6.1858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heller J. S., Kyriakidis D., Fong W. F., Canellakis E. S. Ornithine decarboxylase antizyme is a normal component of uninduced H-35 cells and rat liver. Eur J Biochem. 1977 Dec;81(3):545–550. doi: 10.1111/j.1432-1033.1977.tb11980.x. [DOI] [PubMed] [Google Scholar]
  12. Henningsson S., Rosengren E. Biosynthesis of histamine and putrescine in mice during post-natal development and its hormone dependence. J Physiol. 1975 Feb;245(2):467–479. doi: 10.1113/jphysiol.1975.sp010856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hershko A., Ciechanover A. Mechanisms of intracellular protein breakdown. Annu Rev Biochem. 1982;51:335–364. doi: 10.1146/annurev.bi.51.070182.002003. [DOI] [PubMed] [Google Scholar]
  14. Hietala O. A. Detection of ornithine decarboxylase antizyme in mouse brain. J Neurochem. 1983 Apr;40(4):1174–1177. doi: 10.1111/j.1471-4159.1983.tb08110.x. [DOI] [PubMed] [Google Scholar]
  15. Isomaa V. V., Pajunen A. E., Bardin C. W., Jänne O. A. Ornithine decarboxylase in mouse kidney. Purification, characterization, and radioimmunological determination of the enzyme protein. J Biol Chem. 1983 Jun 10;258(11):6735–6740. [PubMed] [Google Scholar]
  16. Kameji T., Murakami Y., Fujita K., Hayashi S. Purification and some properties of ornithine decarboxylase from rat liver. Biochim Biophys Acta. 1982 Jul 16;717(1):111–117. doi: 10.1016/0304-4165(82)90387-7. [DOI] [PubMed] [Google Scholar]
  17. Laitinen P. H., Huhtinen R. L., Hietala O. A., Pajunen A. E. Ornithine decarboxylase activity in brain regulated by a specific macromolecule, the antizyme. J Neurochem. 1985 Jun;44(6):1885–1891. doi: 10.1111/j.1471-4159.1985.tb07184.x. [DOI] [PubMed] [Google Scholar]
  18. Loeb D., Houben P. W., Bullock L. P. Two forms of ornithine decarboxylase activity in mouse kidney. Mol Cell Endocrinol. 1984 Nov;38(1):67–73. doi: 10.1016/0303-7207(84)90146-1. [DOI] [PubMed] [Google Scholar]
  19. Matsufuji S., Fujita K., Kameji T., Kanamoto R., Murakami Y., Hayashi S. A monoclonal antibody to rat liver ornithine decarboxylase. J Biochem. 1984 Nov;96(5):1525–1530. doi: 10.1093/oxfordjournals.jbchem.a134981. [DOI] [PubMed] [Google Scholar]
  20. McCann P. P., Tardif C., Hornsperger J. M., Böhlen P. Two distinct mechanisms for ornithine decarboxylase regulation by polyamines in rat hepatoma cells. J Cell Physiol. 1979 May;99(2):183–190. doi: 10.1002/jcp.1040990204. [DOI] [PubMed] [Google Scholar]
  21. Mitchell J. L., Mahan D. W., McCann P. P., Qasba P. Dicyclohexylamine effects on HTC cell polyamine content and ornithine decarboxylase activity. Biochim Biophys Acta. 1985 Jul 5;840(3):309–316. doi: 10.1016/0304-4165(85)90210-7. [DOI] [PubMed] [Google Scholar]
  22. Murakami Y., Fujita K., Kameji T., Hayashi S. Accumulation of ornithine decarboxylase-antizyme complex in HMOA cells. Biochem J. 1985 Feb 1;225(3):689–697. doi: 10.1042/bj2250689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Murakami Y., Hayashi S. Role of antizyme in degradation of ornithine decarboxylase in HTC cells. Biochem J. 1985 Mar 15;226(3):893–896. doi: 10.1042/bj2260893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Murakami Y., Kameji T., Hayashi S. Cysteine-dependent inactivation of hepatic ornithine decarboxylase. Biochem J. 1984 Jan 15;217(2):573–580. doi: 10.1042/bj2170573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Murakami Y., Marumo M., Hayashi S. Existence of antizyme and ornithine decarboxylase-antizyme complex in RK13 kidney cells. FEBS Lett. 1986 Apr 7;199(1):49–52. doi: 10.1016/0014-5793(86)81221-2. [DOI] [PubMed] [Google Scholar]
  26. Onoue H., Matsufuji S., Nishiyama M., Murakami Y., Hayashi S. Changes in ornithine decarboxylase and antizyme activities in developing mouse brain. Biochem J. 1988 Mar 15;250(3):797–803. doi: 10.1042/bj2500797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pegg A. E., Conover C., Wrona A. Effects of aliphatic diamines on rat liver ornithine decarboxylase activity. Biochem J. 1978 Mar 15;170(3):651–660. doi: 10.1042/bj1700651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Pegg A. E. Recent advances in the biochemistry of polyamines in eukaryotes. Biochem J. 1986 Mar 1;234(2):249–262. doi: 10.1042/bj2340249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Persson L. Decarboxylation of ornithine and lysine by ornithine decarboxylase from kidneys of testosterone treated mice. Acta Chem Scand B. 1981;35(6):451–459. doi: 10.3891/acta.chem.scand.35b-0451. [DOI] [PubMed] [Google Scholar]
  30. Persson L., Seely J. E., Pegg A. E. Investigation of structure and rate of synthesis of ornithine decarboxylase protein in mouse kidney. Biochemistry. 1984 Jul 31;23(16):3777–3783. doi: 10.1021/bi00311a033. [DOI] [PubMed] [Google Scholar]
  31. Seely J. E., Pegg A. E. Changes in mouse kidney ornithine decarboxylase activity are brought about by changes in the amount of enzyme protein as measured by radioimmunoassay. J Biol Chem. 1983 Feb 25;258(4):2496–2500. [PubMed] [Google Scholar]
  32. Seely J. E., Pegg A. E. Effect of 1,3-diaminopropane on ornithine decarboxylase enzyme protein in thioacetamide-treated rat liver. Biochem J. 1983 Dec 15;216(3):701–707. doi: 10.1042/bj2160701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Seely J. E., Pösö H., Pegg A. E. Effect of androgens on turnover of ornithine decarboxylase in mouse kidney. Studies using labeling of the enzyme by reaction with [14C] alpha-difluoromethylornithine. J Biol Chem. 1982 Jul 10;257(13):7549–7553. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES