Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1988 Sep 1;254(2):477–481. doi: 10.1042/bj2540477

Effects of riboflavin deficiency and clofibrate treatment on the five acyl-CoA dehydrogenases in rat liver mitochondria.

K Veitch 1, J P Draye 1, F Van Hoof 1, H S Sherratt 1
PMCID: PMC1135102  PMID: 3178769

Abstract

Rats were maintained on a riboflavin-deficient diet or on a diet containing clofibrate (0.5%, w/w). The activities of the mitochondrial FAD-dependent straight-chain acyl-CoA dehydrogenases (butyryl-CoA, octanoyl-CoA and palmitoyl-CoA) and the branched-chain acyl-CoA dehydrogenases (isovaleryl-CoA and isobutyryl-CoA) involved in the degradation of branched-chain acyl-CoA esters derived from branched-chain amino acids were assayed in liver mitochondrial extracts prepared in the absence and presence of exogenous FAD. These activities were low in livers from riboflavin-deficient rats (11, 28, 16, 6 and less than 2% of controls respectively) when prepared in the absence of exogenous FAD, and were not restored to control values when prepared in 25 microM-FAD (29, 47, 28, 7 and 17%). Clofibrate feeding increased the activities of butyryl-CoA, octanoyl-CoA and palmitoyl-CoA dehydrogenases (by 48, 116 and 98% of controls respectively), but not, by contrast, the activities of isovaleryl-CoA and isobutyryl-CoA dehydrogenases (62 and 102% of controls respectively). The mitochondrial fractions from riboflavin-deficient and from clofibrate-fed rats oxidized palmitoylcarnitine in State 3 at rates of 32 and 163% respectively of those from control rats.

Full text

PDF
477

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billington D., Osmundsen H., Sherratt H. S. Mechanisms of the metabolic disturbances caused by hypoglycin and by pent-4-enoic acid. In vitro studies. Biochem Pharmacol. 1978;27(24):2879–2890. doi: 10.1016/0006-2952(78)90204-6. [DOI] [PubMed] [Google Scholar]
  2. Causey A. G., Middleton B., Bartlett K. A study of the metabolism of [U-14C]3-methyl-2-oxopentanoate by rat liver mitochondria using h.p.l.c. with continuous on-line monitoring of radioactive intact acyl-coenzyme A intermediates. Biochem J. 1986 Apr 15;235(2):343–350. doi: 10.1042/bj2350343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fisher H. F. L-Glutamate dehydrogenase from bovine liver. Methods Enzymol. 1985;113:16–27. doi: 10.1016/s0076-6879(85)13006-5. [DOI] [PubMed] [Google Scholar]
  4. Furuta S., Miyazawa S., Hashimoto T. Induction of acyl-CoA dehydrogenases and electron transfer flavoprotein and their roles in fatty acid oxidation in rat liver mitochondria. J Biochem. 1981 Dec;90(6):1751–1756. doi: 10.1093/oxfordjournals.jbchem.a133652. [DOI] [PubMed] [Google Scholar]
  5. Furuta S., Miyazawa S., Hashimoto T. Purification and properties of rat liver acyl-CoA dehydrogenases and electron transfer flavoprotein. J Biochem. 1981 Dec;90(6):1739–1750. doi: 10.1093/oxfordjournals.jbchem.a133651. [DOI] [PubMed] [Google Scholar]
  6. Gear A. R., Albert A. D., Bednarek J. M. The effect of the hypocholesterolemic drug clofibrate on liver mitochondrial biogenesis. A role for neutral mitochondrial proteases. J Biol Chem. 1974 Oct 25;249(20):6495–6504. [PubMed] [Google Scholar]
  7. Goodman S. I. Organic aciduria in the riboflavin-deficient rat. Am J Clin Nutr. 1981 Nov;34(11):2434–2437. doi: 10.1093/ajcn/34.11.2434. [DOI] [PubMed] [Google Scholar]
  8. Hoppel C., Cooper C. The action of digitonin on rat liver mitochondria. The effects on enzyme content. Biochem J. 1968 Apr;107(3):367–375. doi: 10.1042/bj1070367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoppel C., DiMarco J. P., Tandler B. Riboflavin and rat hepatic cell structure and function. Mitochondrial oxidative metabolism in deficiency states. J Biol Chem. 1979 May 25;254(10):4164–4170. [PubMed] [Google Scholar]
  10. Ikeda Y., Dabrowski C., Tanaka K. Separation and properties of five distinct acyl-CoA dehydrogenases from rat liver mitochondria. Identification of a new 2-methyl branched chain acyl-CoA dehydrogenase. J Biol Chem. 1983 Jan 25;258(2):1066–1076. [PubMed] [Google Scholar]
  11. Ikeda Y., Tanaka K. Purification and characterization of 2-methyl-branched chain acyl coenzyme A dehydrogenase, an enzyme involved in the isoleucine and valine metabolism, from rat liver mitochondria. J Biol Chem. 1983 Aug 10;258(15):9477–9487. [PubMed] [Google Scholar]
  12. Ikeda Y., Tanaka K. Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria. J Biol Chem. 1983 Jan 25;258(2):1077–1085. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lazarow P. B. Rat liver peroxisomes catalyze the beta oxidation of fatty acids. J Biol Chem. 1978 Mar 10;253(5):1522–1528. [PubMed] [Google Scholar]
  15. Ross N. S., Hoppel C. L. Acyl-CoA dehydrogenase activity in the riboflavin-deficient rat. Effects of starvation. Biochem J. 1987 Jun 1;244(2):387–391. doi: 10.1042/bj2440387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sakurai T., Miyazawa S., Furuta S., Hashimoto T. Riboflavin deficiency and beta-oxidation systems in rat liver. Lipids. 1982 Sep;17(9):598–604. doi: 10.1007/BF02535365. [DOI] [PubMed] [Google Scholar]
  17. Tanaka K. On the mode of action of hypoglycin A. 3. Isolation and identification of cis-4-decene-1,10-dioic, cis, cis-4,7-decadiene-1,10-dioic, cis-4-octene-1,8-dioic, glutaric, and adipic acids, N-(methylenecyclopropyl)acetylglycine, and N-isovalerylglycine from urine of hypoglycin A-treated rats. J Biol Chem. 1972 Dec 10;247(23):7465–7478. [PubMed] [Google Scholar]
  18. Tillotson J. A., Sauberlich H. E. Effect of riboflavin depletion and repletion on the erythrocyte glutathione reductase in the rat. J Nutr. 1971 Nov;101(11):1459–1466. doi: 10.1093/jn/101.11.1459. [DOI] [PubMed] [Google Scholar]
  19. Vamecq J., de Hoffmann E., Van Hoof F. The microsomal dicarboxylyl-CoA synthetase. Biochem J. 1985 Sep 15;230(3):683–693. doi: 10.1042/bj2300683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Van Hoof F., Hue L., Vamecq J., Sherratt H. S. Protection of rats by clofibrate against the hypoglycaemic and toxic effects of hypoglycin and pent-4-enoate. An ultrastructural and biochemical study. Biochem J. 1985 Jul 15;229(2):387–397. doi: 10.1042/bj2290387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Veitch R. K., Sherratt H. S., Bartlett K. Organic aciduria in rats made resistant to hypoglycin toxicity by pretreatment with clofibrate. Biochem J. 1987 Sep 15;246(3):775–778. doi: 10.1042/bj2460775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wenz A., Thorpe C., Ghisla S. Inactivation of general acyl-CoA dehydrogenase from pig kidney by a metabolite of hypoglycin A. J Biol Chem. 1981 Oct 10;256(19):9809–9812. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES