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. 1988 Sep 15;254(3):923–925. doi: 10.1042/bj2540923

Kinetic characterization of the acyl-enzyme mechanism for beta-lactamase I.

M T Martin 1, S G Waley 1
PMCID: PMC1135174  PMID: 3143353

Abstract

beta-Lactamase I catalyses the hydrolysis of penicillins by an acyl-enzyme mechanism. A procedure was developed for determining the rate constants for the acylation and deacylation steps for the good substrates benzylpenicillin and phenoxymethylpenicillin; this depends on determining the fraction of enzyme that is present as acyl-enzyme in the steady state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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