Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1988 Oct 1;255(1):365–368.

Probable detection of kil peptide derived from colicin E1 plasmid in the envelope fraction of Escherichia coli HB101 carrying pEAP31.

R Aono 1
PMCID: PMC1135232  PMID: 3058121

Abstract

Escherichia coli carrying plasmid pEAP31 produces extracellularly alkalophilic Bacillus penicillinase encoded on the plasmid. The extracellular production has been suggested to be caused by activation of dormant colicin E1 kil gene. Two peptides that could be respectively precursor and mature products of colicin E1 kil gene were detected on an SDS/polyacrylamide gel. One of the peptides (Mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organism when envelope proteins were subjected to differential solubilization. The other (Mr 3500), which was a mature peptide, was detected in the outer-membrane fraction of the organism. The mature peptide was only detected in the envelope of cells releasing the penicillinase transiently accumulated in the periplasm into the culture medium.

Full text

PDF
365

Images in this article

367Fig. 1.
on p.367
367Fig. 2.
on p.367

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolivar F., Backman K. Plasmids of Escherichia coli as cloning vectors. Methods Enzymol. 1979;68:245–267. doi: 10.1016/0076-6879(79)68018-7. [DOI] [PubMed] [Google Scholar]
  2. Cavard D., Baty D., Howard S. P., Verheij H. M., Lazdunski C. Lipoprotein nature of the colicin A lysis protein: effect of amino acid substitutions at the site of modification and processing. J Bacteriol. 1987 May;169(5):2187–2194. doi: 10.1128/jb.169.5.2187-2194.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hakkaart M. J., Veltkamp E., Nijkamp H. J. Protein H encoded by plasmid Clo DF13 involved in lysis of the bacterial host. I. Localisation of the gene and identification and subcellular localisation of the gene H product. Mol Gen Genet. 1981;183(2):318–325. doi: 10.1007/BF00270635. [DOI] [PubMed] [Google Scholar]
  5. Hussain M., Ichihara S., Mizushima S. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin. J Biol Chem. 1980 Apr 25;255(8):3707–3712. [PubMed] [Google Scholar]
  6. Kobayashi T., Kato C., Kudo T., Horikoshi K. Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane is caused by insertional activation of the kil gene in plasmid pMB9. J Bacteriol. 1986 Jun;166(3):728–732. doi: 10.1128/jb.166.3.728-732.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Pugsley A. P., Cole S. T. An unmodified form of the ColE2 lysis protein, an envelope lipoprotein, retains reduced ability to promote colicin E2 release and lysis of producing cells. J Gen Microbiol. 1987 Sep;133(9):2411–2420. doi: 10.1099/00221287-133-9-2411. [DOI] [PubMed] [Google Scholar]
  9. Pugsley A. P., Schwartz M. A genetic approach to the study of mitomycin-induced lysis of Escherichia coli K-12 strains which produce colicin E2. Mol Gen Genet. 1983;190(3):366–372. doi: 10.1007/BF00331060. [DOI] [PubMed] [Google Scholar]
  10. Pugsley A. P., Schwartz M. Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase. EMBO J. 1984 Oct;3(10):2393–2397. doi: 10.1002/j.1460-2075.1984.tb02145.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sabik J. F., Suit J. L., Luria S. E. cea-kil operon of the ColE1 plasmid. J Bacteriol. 1983 Mar;153(3):1479–1485. doi: 10.1128/jb.153.3.1479-1485.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sawai T., Takahashi I., Yamagishi S. Iodometric assay method for beta-lactamase with various beta-lactam antibiotics as substrates. Antimicrob Agents Chemother. 1978 Jun;13(6):910–913. doi: 10.1128/aac.13.6.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Suit J. L., Fan M. L., Sabik J. F., Labarre R., Luria S. E. Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids. Proc Natl Acad Sci U S A. 1983 Jan;80(2):579–583. doi: 10.1073/pnas.80.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sutton B. J., Artymiuk P. J., Cordero-Borboa A. E., Little C., Phillips D. C., Waley S. G. An X-ray-crystallographic study of beta-lactamase II from Bacillus cereus at 0.35 nm resolution. Biochem J. 1987 Nov 15;248(1):181–188. doi: 10.1042/bj2480181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Watson R. J., Visentin L. P. Cloning of the ColE3-CA38 colicin and immunity genes and identification of a plasmid region which enhances colicin production. Gene. 1982 Sep;19(2):191–200. doi: 10.1016/0378-1119(82)90006-3. [DOI] [PubMed] [Google Scholar]
  16. Zhang S. P., Faro A., Zubay G. Mitomycin-induced lethality of Escherichia coli cells containing the ColE1 Plasmid: involvement of the kil gene. J Bacteriol. 1985 Jul;163(1):174–179. doi: 10.1128/jb.163.1.174-179.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES