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. 1988 Oct 15;255(2):741–743.

Cyclic AMP induces metallothionein gene expression in rat hepatocytes but not in rat kidney.

V L Nebes 1, D DeFranco 1, S M Morris Jr 1
PMCID: PMC1135289  PMID: 2849426

Abstract

Cyclic AMP analogues induced expression of metallothionein-I (MT-I) mRNA in adult rat liver and in rat hepatocytes cultured in serum-free medium. This induction occurred via an increased rate of transcription of the MT-I gene. The effect of cyclic AMP analogues was tissue-specific since no induction of MT-I mRNA occurred in rat kidney. Induction of MT-I mRNA by a combination of cyclic AMP analogue and dexamethasone was additive in liver and cultured hepatocytes, indicating that induction occurred via independent regulatory pathways.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen R. D., Birren B. W., Taplitz S. J., Herschman H. R. Rat metallothionein-1 structural gene and three pseudogenes, one of which contains 5'-regulatory sequences. Mol Cell Biol. 1986 Jan;6(1):302–314. doi: 10.1128/mcb.6.1.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birren B. W., Herschman H. R. Regulation of the rat metallothionein-I gene by sodium butyrate. Nucleic Acids Res. 1986 Jan 24;14(2):853–867. doi: 10.1093/nar/14.2.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bond J. F., Robinson G. S., Farmer S. R. Differential expression of two neural cell-specific beta-tubulin mRNAs during rat brain development. Mol Cell Biol. 1984 Jul;4(7):1313–1319. doi: 10.1128/mcb.4.7.1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Comb M., Birnberg N. C., Seasholtz A., Herbert E., Goodman H. M. A cyclic AMP- and phorbol ester-inducible DNA element. 1986 Sep 25-Oct 1Nature. 323(6086):353–356. doi: 10.1038/323353a0. [DOI] [PubMed] [Google Scholar]
  6. Cousins R. J. Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Physiol Rev. 1985 Apr;65(2):238–309. doi: 10.1152/physrev.1985.65.2.238. [DOI] [PubMed] [Google Scholar]
  7. Cousins R. J., Dunn M. A., Leinart A. S., Yedinak K. C., DiSilvestro R. A. Coordinate regulation of zinc metabolism and metallothionein gene expression in rats. Am J Physiol. 1986 Dec;251(6 Pt 1):E688–E694. doi: 10.1152/ajpendo.1986.251.6.E688. [DOI] [PubMed] [Google Scholar]
  8. Durnam D. M., Hoffman J. S., Quaife C. J., Benditt E. P., Chen H. Y., Brinster R. L., Palmiter R. D. Induction of mouse metallothionein-I mRNA by bacterial endotoxin is independent of metals and glucocorticoid hormones. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1053–1056. doi: 10.1073/pnas.81.4.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Etzel K. R., Cousins R. J. Hormonal regulation of liver metallothionein zinc: independent and synergistic action of glucagon and glucocorticoids. Proc Soc Exp Biol Med. 1981 Jun;167(2):233–236. doi: 10.3181/00379727-167-41155. [DOI] [PubMed] [Google Scholar]
  10. Friedman R. L., Stark G. R. alpha-Interferon-induced transcription of HLA and metallothionein genes containing homologous upstream sequences. Nature. 1985 Apr 18;314(6012):637–639. doi: 10.1038/314637a0. [DOI] [PubMed] [Google Scholar]
  11. Hager L. J., Palmiter R. D. Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids. Nature. 1981 May 28;291(5813):340–342. doi: 10.1038/291340a0. [DOI] [PubMed] [Google Scholar]
  12. Hamer D. H. Metallothionein. Annu Rev Biochem. 1986;55:913–951. doi: 10.1146/annurev.bi.55.070186.004405. [DOI] [PubMed] [Google Scholar]
  13. Heldin C. H., Wasteson A., Westermark B. Growth of normal human glial cells in a defined medium containing platelet-derived growth factor. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6611–6615. doi: 10.1073/pnas.77.11.6611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hidalgo H. A., Koppa V., Bryan S. E. Induction of cadmium-thionein in isolated rat liver cells. Biochem J. 1978 Feb 15;170(2):219–225. doi: 10.1042/bj1700219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Imagawa M., Chiu R., Karin M. Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP. Cell. 1987 Oct 23;51(2):251–260. doi: 10.1016/0092-8674(87)90152-8. [DOI] [PubMed] [Google Scholar]
  16. Imbra R. J., Karin M. Metallothionein gene expression is regulated by serum factors and activators of protein kinase C. Mol Cell Biol. 1987 Apr;7(4):1358–1363. doi: 10.1128/mcb.7.4.1358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Karin M., Herschman H. R. Dexamethasone stimulation of metallothionein synthesis in HeLa cell cultures. Science. 1979 Apr 13;204(4389):176–177. doi: 10.1126/science.432639. [DOI] [PubMed] [Google Scholar]
  18. Mayo K. E., Palmiter R. D. Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells. J Biol Chem. 1981 Mar 25;256(6):2621–2624. [PubMed] [Google Scholar]
  19. Montminy M. R., Bilezikjian L. M. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature. 1987 Jul 9;328(6126):175–178. doi: 10.1038/328175a0. [DOI] [PubMed] [Google Scholar]
  20. Montminy M. R., Sevarino K. A., Wagner J. A., Mandel G., Goodman R. H. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682–6686. doi: 10.1073/pnas.83.18.6682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Morris S. M., Jr, Moncman C. L., Rand K. D., Dizikes G. J., Cederbaum S. D., O'Brien W. E. Regulation of mRNA levels for five urea cycle enzymes in rat liver by diet, cyclic AMP, and glucocorticoids. Arch Biochem Biophys. 1987 Jul;256(1):343–353. doi: 10.1016/0003-9861(87)90455-3. [DOI] [PubMed] [Google Scholar]
  22. Nebes V. L., Morris S. M., Jr Induction of mRNA for phosphoenolpyruvate carboxykinase (GTP) by dexamethasone in cultured rat hepatocytes requires on-going protein synthesis. Biochem J. 1987 Aug 15;246(1):237–240. doi: 10.1042/bj2460237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nebes V. L., Morris S. M., Jr Regulation of messenger ribonucleic acid levels for five urea cycle enzymes in cultured rat hepatocytes. Requirements for cyclic adenosine monophosphate, glucocorticoids, and ongoing protein synthesis. Mol Endocrinol. 1988 May;2(5):444–451. doi: 10.1210/mend-2-5-444. [DOI] [PubMed] [Google Scholar]
  24. Short J. M., Wynshaw-Boris A., Short H. P., Hanson R. W. Characterization of the phosphoenolpyruvate carboxykinase (GTP) promoter-regulatory region. II. Identification of cAMP and glucocorticoid regulatory domains. J Biol Chem. 1986 Jul 25;261(21):9721–9726. [PubMed] [Google Scholar]
  25. Silver B. J., Bokar J. A., Virgin J. B., Vallen E. A., Milsted A., Nilson J. H. Cyclic AMP regulation of the human glycoprotein hormone alpha-subunit gene is mediated by an 18-base-pair element. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2198–2202. doi: 10.1073/pnas.84.8.2198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tsukada T., Fink J. S., Mandel G., Goodman R. H. Identification of a region in the human vasoactive intestinal polypeptide gene responsible for regulation by cyclic AMP. J Biol Chem. 1987 Jun 25;262(18):8743–8747. [PubMed] [Google Scholar]
  27. Van Winkle W. B., Tate C. A., Bick R. J., Entman M. L. Nucleotide triphosphate utilization by cardiac and skeletal muscle sarcoplasmic reticulum. Evidence for a hydrolysis cycle not coupled to intermediate acyl phosphate formation and calcium translocation. J Biol Chem. 1981 Mar 10;256(5):2268–2274. [PubMed] [Google Scholar]

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