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. 1988 Nov 15;256(1):265–270. doi: 10.1042/bj2560265

Role of human serum biotinidase as biotin-binding protein.

J Chauhan 1, K Dakshinamurti 1
PMCID: PMC1135397  PMID: 3223902

Abstract

Biotinidase shows two binding sites for biotin, with Kd = 59 and 3 nM respectively, and requires tryptophan and cysteine residues of the biotinidase protein for biotin-binding activity. Analysis of human serum by various column-chromatographic techniques indicates that biotinidase is the only protein which exchanges with labelled (+)-biotin. It was shown previously that epileptic patients receiving a high average dose of anticonvulsants (containing a carbamide group) have lower biotin concentrations than those receiving a low dose. We have shown in human serum and with purified biotinidase that these anticonvulsant drugs compete with biotin for binding to the protein moiety.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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