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. 1988 Nov 15;256(1):299–302. doi: 10.1042/bj2560299

Energetics of renal Na+ and H+/L-alanine co-transport systems.

H Jessen 1, H Vorum 1, K E Jørgensen 1, M I Sheikh 1
PMCID: PMC1135403  PMID: 2851984

Abstract

The stoichiometric properties of Na+- and H+-dependent L-alanine transporters recently identified in luminal-membrane vesicles prepared from proximal convoluted tubules (pars convoluta) and proximal straight tubules (pars recta) of rabbit kidney were studied. We provide indirect evidence suggesting that one Na+ and one H+ ion are co-transported with the L-alanine molecule via Na+-dependent and H+-dependent transport systems located in vesicles from pars convoluta. Furthermore, our experimental data suggest that both the high-affinity and the low-affinity Na+-dependent L-alanine transport systems of pars recta vesicles operate with a 1:1 stoichiometry.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hopfer U., Nelson K., Perrotto J., Isselbacher K. J. Glucose transport in isolated brush border membrane from rat small intestine. J Biol Chem. 1973 Jan 10;248(1):25–32. [PubMed] [Google Scholar]
  2. Jessen H., Vorum H., Jørgensen K. E., Sheikh M. I. Characteristics of D-alanine transport by luminal membrane vesicles from pars convoluta and pars recta of rabbit proximal tubule. Biochim Biophys Acta. 1988 Jul 21;942(2):262–270. doi: 10.1016/0005-2736(88)90028-4. [DOI] [PubMed] [Google Scholar]
  3. Jørgensen K. E., Sheikh M. I. Renal transport of neutral amino acids. Cation-dependent uptake of L-alanine by luminal-membrane vesicles. Biochem J. 1987 Dec 1;248(2):533–538. doi: 10.1042/bj2480533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kragh-Hansen U., Røigaard-Petersen H., Jacobsen C., Sheikh M. I. Renal transport of neutral amino acids. Tubular localization of Na+-dependent phenylalanine- and glucose-transport systems. Biochem J. 1984 May 15;220(1):15–24. doi: 10.1042/bj2200015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kragh-Hansen U., Røigaard-Petersen H., Sheikh M. I. Segmental localization of the rabbit renal proximal tubular Na+-H+ exchange system. Am J Physiol. 1985 Nov;249(5 Pt 2):F704–F712. doi: 10.1152/ajprenal.1985.249.5.F704. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Murer H., Gmaj P. Transport studies in plasma membrane vesicles isolated from renal cortex. Kidney Int. 1986 Aug;30(2):171–186. doi: 10.1038/ki.1986.169. [DOI] [PubMed] [Google Scholar]
  8. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  9. Sheikh M. I., Kragh-Hansen U., Jørgensen K. E., Røigaard-Petersen H. An efficient method for the isolation and separation of basolateral-membrane and luminal-membrane vesicles from rabbit kidney cortex. Biochem J. 1982 Nov 15;208(2):377–382. doi: 10.1042/bj2080377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Turner R. J., Moran A. Further studies of proximal tubular brush border membrane D-glucose transport heterogeneity. J Membr Biol. 1982;70(1):37–45. doi: 10.1007/BF01871587. [DOI] [PubMed] [Google Scholar]
  11. Turner R. J., Moran A. Heterogeneity of sodium-dependent D-glucose transport sites along the proximal tubule: evidence from vesicle studies. Am J Physiol. 1982 Apr;242(4):F406–F414. doi: 10.1152/ajprenal.1982.242.4.F406. [DOI] [PubMed] [Google Scholar]
  12. Turner R. J., Moran A. Stoichiometric studies of the renal outer cortical brush border membrane D-glucose transporter. J Membr Biol. 1982;67(1):73–80. doi: 10.1007/BF01868649. [DOI] [PubMed] [Google Scholar]
  13. Vorum H., Jessen H., Jørgensen K. E., Sheikh M. I. Mechanism of transport of L-alanine by luminal-membrane vesicles from pars recta of rabbit proximal tubule. FEBS Lett. 1988 Jan 18;227(1):35–38. doi: 10.1016/0014-5793(88)81408-x. [DOI] [PubMed] [Google Scholar]

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