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. 1988 Dec 1;256(2):537–541. doi: 10.1042/bj2560537

Influence of interferon-gamma and extracellular tryptophan on indoleamine 2,3-dioxygenase activity in T24 cells as determined by a non-radiometric assay.

E R Werner 1, G Werner-Felmayer 1, D Fuchs 1, A Hausen 1, G Reibnegger 1, H Wachter 1
PMCID: PMC1135443  PMID: 3146975

Abstract

The indoleamine 2,3-dioxygenase (EC 1.13.11.17) activity in human T24 cells has been investigated in cell extracts by using a non-radioactive assay. It is enhanced in a dose-dependent manner up to 25-fold by interferon-gamma. The maximum reaction velocity is increased rather than the Km, which remains at 4 mumol/l. Induction of activity starts 3 h after stimulation and reaches a plateau at 21-48 h. Decreased stimulation was observed in the presence of high L-tryptophan concentrations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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