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Niacinamide interaction with DNA amplicon by gel electrophoresis and qPCR. (A) Agarose gel image of DNA fragment incubated with niacinamide at increasing concentrations. (B) qPCR assay results (Cq = cycle of amplification, Tm = melting temperature of the amplicon) with amplicon’s relevant primers. (C) Clean PCR products of amplified DNA treated with increasing concentrations of niacinamide (5–20%), arrows represent fragmented DNA product.
