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. 1989 Feb 1;257(3):673–678. doi: 10.1042/bj2570673

Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process.

B Crabtree 1, M J Souter 1, S E Anderson 1
PMCID: PMC1135640  PMID: 2564776

Abstract

By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.

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Selected References

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