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. 1989 Feb 1;257(3):783–787. doi: 10.1042/bj2570783

Reconstitution of cytochrome c oxidase in phospholipid vesicles containing polyvinylic polymers.

P Sarti 1, G Antonini 1, F Malatesta 1, B Vallone 1, S Villaschi 1, M Brunori 1, R C Hider 1, K Hamed 1
PMCID: PMC1135656  PMID: 2539096

Abstract

Cytochrome c oxidase was reconstituted in phospholipid vesicles in the presence of highly hydrophobic poly(vinyl alkanoate) polymers. Electron-microscopy observations demonstrated that polymer interaction with the lipid phase induces vesicles to adopt smaller diameters than those typical of standard proteoliposomes. Functional characterization of these polymer-proteoliposome structures indicates that the reconstitution of the enzyme proceeds efficiently without causing either scrambling of the protein orientation in the membrane or loss of respiratory control. A clear dependence of respiratory control ratio on vesicle size was also demonstrated, which is in agreement with a previous model proposed for control of activity of cytochrome c oxidase vesicles [Brunori, Sarti, Colosimo, Antonini, Malatesta, Jones & Wilson (1985) EMBO J. 4, 2365-2368].

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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