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. 1989 Feb 1;257(3):859–863. doi: 10.1042/bj2570859

Nitroreductase activity of NADH dehydrogenase of the respiratory redox chain.

G E Smyth 1, B A Orsi 1
PMCID: PMC1135667  PMID: 2494990

Abstract

1. An NADH-dependent nitroreductase from the inner membrane of ox liver mitochondria copurified with Complex I of the respiratory redox chain (NADH:ubiquinone oxidoreductase, EC 1.6.5.3). 2. The corresponding nitroreductase from ox heart mitochondria co-purified with the NADH-cytochrome c reductase of Mahler, Sarkar & Vernon [(1952) J. Biol. Chem. 199, 585-597] [NADH: (acceptor) oxidoreductase, EC 1.6.99.3], a component of Complex I that contains the FMN. 3. The mitochondrial nitroreductase activity is attributed to the flavoprotein component of Complex I.

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Selected References

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  1. Baugh R. F., King T. E. Purification, properties and reconstitutive activity of a DPHN dehydrogenase. Biochem Biophys Res Commun. 1972 Dec 4;49(5):1165–1173. doi: 10.1016/0006-291x(72)90591-8. [DOI] [PubMed] [Google Scholar]
  2. Biggs D. R., Hauber J., Singer T. P. Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. XII. Interrelations of reduced nicotinamide adenine dinucleotide-coenzyme Q reductase. J Biol Chem. 1967 Oct 25;242(20):4563–4567. [PubMed] [Google Scholar]
  3. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. George C. L., Ferguson S. J. Immunochemical identification of a two-subunit NADH-ubiquinone oxidoreductase from Paracoccus denitrificans. Eur J Biochem. 1984 Sep 17;143(3):567–573. doi: 10.1111/j.1432-1033.1984.tb08407.x. [DOI] [PubMed] [Google Scholar]
  5. Harada N., Omura T. Participation of cytochrome P-450 in the reduction of nitro compounds by rat liver microsomes. J Biochem. 1980 May;87(5):1539–1554. doi: 10.1093/oxfordjournals.jbchem.a132895. [DOI] [PubMed] [Google Scholar]
  6. Hatefi Y. Introduction--preparation and properties of the enzymes and enzymes complexes of the mitochondrial oxidative phosphorylation system. Methods Enzymol. 1978;53:3–4. doi: 10.1016/s0076-6879(78)53004-8. [DOI] [PubMed] [Google Scholar]
  7. Hatefi Y. Preparation and properties of NADH: ubiquinone oxidoreductase (complexI), EC 1.6.5.3. Methods Enzymol. 1978;53:11–14. doi: 10.1016/s0076-6879(78)53006-1. [DOI] [PubMed] [Google Scholar]
  8. Hatefi Y., Stiggall D. L. Preparation and properties of NADH: cytochrome c oxidoreductase (complex I--III). Methods Enzymol. 1978;53:5–10. doi: 10.1016/s0076-6879(78)53005-x. [DOI] [PubMed] [Google Scholar]
  9. Kato R., Takahashi A., Oshima T. Characteristics of nitro reduction of the carcinogenic agent, 4-nitroINOLONE N-oxide. Biochem Pharmacol. 1970 Jan;19(1):45–55. doi: 10.1016/0006-2952(70)90328-x. [DOI] [PubMed] [Google Scholar]
  10. Köchli H. W., Wermuth B., von Wartburg J. P. Characterization of a mitochondrial NADH-dependent nitro reductase from rat brain. Biochim Biophys Acta. 1980 Dec 4;616(2):133–142. doi: 10.1016/0005-2744(80)90131-x. [DOI] [PubMed] [Google Scholar]
  11. MAHLER H. R., SARKAR N. K., VERNON L. P. Studies on diphosphopyridine nucleotide-cytochrome c reductase. II. Purification and properties. J Biol Chem. 1952 Dec;199(2):585–597. [PubMed] [Google Scholar]
  12. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  13. Mitchard M. Bioreduction of organic nitrogen. Xenobiotica. 1971 Jul-Oct;1(4):469–481. doi: 10.3109/00498257109041512. [DOI] [PubMed] [Google Scholar]
  14. Moreno S. N., Mason R. P., Docampo R. Reduction of nifurtimox and nitrofurantoin to free radical metabolites by rat liver mitochondria. Evidence of an outer membrane-located nitroreductase. J Biol Chem. 1984 May 25;259(10):6298–6305. [PubMed] [Google Scholar]
  15. Owen P., Salton M. R. Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3711–3715. doi: 10.1073/pnas.72.9.3711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ryle C. M., Tipton K. F. Subcellular localization of aldehyde reductase activities in ox brain. Biochem J. 1981 Sep 1;197(3):715–720. doi: 10.1042/bj1970715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tatsumi K., Kitamura S., Yoshimura H. Reduction of nitrofuran derivatives by xanthine oxidase and microsomes. Isolation and identification of reduction products. Arch Biochem Biophys. 1976 Jul;175(1):131–137. doi: 10.1016/0003-9861(76)90491-4. [DOI] [PubMed] [Google Scholar]
  19. Tsai C. S. Nitroreductase activity of heart lipoamide dehydrogenase. Biochem J. 1987 Mar 1;242(2):447–452. doi: 10.1042/bj2420447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wolpert M. K., Althaus J. R., Johns D. G. Nitroreductase activity of mammalian liver aldehyde oxidase. J Pharmacol Exp Ther. 1973 May;185(2):202–213. [PubMed] [Google Scholar]

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