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. 1989 Feb 1;257(3):921–924. doi: 10.1042/bj2570921

Structure of the human lactate dehydrogenase B gene.

T Takeno 1, S S Li 1
PMCID: PMC1135676  PMID: 2930497

Abstract

Human genomic clones containing parts of the lactate dehydrogenase B (LDH-B) gene (approx. 25 kb in length) were isolated and characterized. The protein-coding sequence of human LDH-B gene is interrupted by six introns at codons nos. 42-43, 82, 140, 198, 237 and 278-279, and the positions of these introns are homologous to those of LDH-A genes from man and mouse. The 5' non-coding region of human LDH-B gene is interrupted by an intron six nucleotide residues upstream of the ATG translation-initiation site, whereas those of human and mouse LDH-A genes are interrupted at 24 nucleotide residues 5' to the ATG initiation codon. As is the case of LDH-A genes from man and mouse, there is no intron in the 3' non-coding region of human LDH-B gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chung F. Z., Tsujibo H., Bhattacharyya U., Sharief F. S., LI S. S. Genomic organization of human lactate dehydrogenase-A gene. Biochem J. 1985 Nov 1;231(3):537–541. doi: 10.1042/bj2310537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edwards Y. H., Povey S., LeVan K. M., Driscoll C. E., Millan J. L., Goldberg E. Locus determining the human sperm-specific lactate dehydrogenase, LDHC, is syntenic with LDHA. Dev Genet. 1987;8(4):219–232. doi: 10.1002/dvg.1020080406. [DOI] [PubMed] [Google Scholar]
  4. Fukasawa K. M., Li S. S. Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes. Genetics. 1987 May;116(1):99–105. doi: 10.1093/genetics/116.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holmes R. S. Evolution of lactate dehydrogenase genes. FEBS Lett. 1972 Nov 15;28(1):51–55. doi: 10.1016/0014-5793(72)80675-6. [DOI] [PubMed] [Google Scholar]
  6. Hou E. W., Li S. S. Cyclic AMP-induced expression of the mouse lactate dehydrogenase-A promoter-cat fusion gene in Chinese hamster ovary wild-type cells, but not in cAMP-dependent protein kinase mutant cells. Biochem Biophys Res Commun. 1987 Aug 31;147(1):501–505. doi: 10.1016/s0006-291x(87)80149-3. [DOI] [PubMed] [Google Scholar]
  7. Jungmann R. A., Derda D. F., Kelley D. C., Miles M. F., Milkowski D., Schweppe J. S. Regulation of lactate dehydrogenase gene expression. Isozymes Curr Top Biol Med Res. 1983;7:161–174. [PubMed] [Google Scholar]
  8. Kanno T., Sudo K., Kitamura M., Miwa S., Ichiyama A., Nishimura Y. Lactate dehydrogenase A-subunit and B-subunit deficiencies: comparison of the physiological roles of LDH isozymes. Isozymes Curr Top Biol Med Res. 1983;7:131–150. [PubMed] [Google Scholar]
  9. Li S. S., Feldmann R. J., Okabe M., Pan Y. C. Molecular features and immunological properties of lactate dehydrogenase C4 isozymes from mouse and rat testes. J Biol Chem. 1983 Jun 10;258(11):7017–7028. [PubMed] [Google Scholar]
  10. Li S. S., Fitch W. M., Pan Y. C., Sharief F. S. Evolutionary relationships of vertebrate lactate dehydrogenase isozymes A4 (muscle), B4 (heart), and C4 (testis). J Biol Chem. 1983 Jun 10;258(11):7029–7032. [PubMed] [Google Scholar]
  11. Li S. S., Luedemann M., Sharief F. S., Takano T., Deaven L. L. Mapping of human lactate dehydrogenase-A, -B, and -C genes and their related sequences: the gene for LDHC is located with that for LDHA on chromosome 11. Cytogenet Cell Genet. 1988;48(1):16–18. doi: 10.1159/000132578. [DOI] [PubMed] [Google Scholar]
  12. Li S. S., Tiano H. F., Fukasawa K. M., Yagi K., Shimizu M., Sharief F. S., Nakashima Y., Pan Y. E. Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme. Eur J Biochem. 1985 Jun 3;149(2):215–225. doi: 10.1111/j.1432-1033.1985.tb08914.x. [DOI] [PubMed] [Google Scholar]
  13. Markert C. L., Shaklee J. B., Whitt G. S. Evolution of a gene. Multiple genes for LDH isozymes provide a model of the evolution of gene structure, function and regulation. Science. 1975 Jul 11;189(4197):102–114. doi: 10.1126/science.1138367. [DOI] [PubMed] [Google Scholar]
  14. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Montminy M. R., Sevarino K. A., Wagner J. A., Mandel G., Goodman R. H. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682–6686. doi: 10.1073/pnas.83.18.6682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mount S. M. A catalogue of splice junction sequences. Nucleic Acids Res. 1982 Jan 22;10(2):459–472. doi: 10.1093/nar/10.2.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pan Y. C., Sharief F. S., Okabe M., Huang S., Li S. S. Amino acid sequence studies on lactate dehydrogenase C4 isozymes from mouse and rat testes. J Biol Chem. 1983 Jun 10;258(11):7005–7016. [PubMed] [Google Scholar]
  18. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  19. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  20. Sakai I., Sharief F. S., Li S. S. Molecular cloning and nucleotide sequence of the cDNA for sperm-specific lactate dehydrogenase-C from mouse. Biochem J. 1987 Mar 1;242(2):619–622. doi: 10.1042/bj2420619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sakai I., Sharief F. S., Pan Y. C., Li S. S. The cDNA and protein sequences of human lactate dehydrogenase B. Biochem J. 1987 Dec 15;248(3):933–936. doi: 10.1042/bj2480933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sharp P. A. Speculations on RNA splicing. Cell. 1981 Mar;23(3):643–646. doi: 10.1016/0092-8674(81)90425-6. [DOI] [PubMed] [Google Scholar]
  24. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  25. Tsujibo H., Tiano H. F., Li S. S. Nucleotide sequences of the cDNA and an intronless pseudogene for human lactate dehydrogenase-A isozyme. Eur J Biochem. 1985 Feb 15;147(1):9–15. doi: 10.1111/j.1432-1033.1985.tb08711.x. [DOI] [PubMed] [Google Scholar]

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