Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1995 Jul 1;309(Pt 1):321–324. doi: 10.1042/bj3090321

Aminoacyl-tRNA synthetase complex in Saccharomyces cerevisiae.

C L Harris 1, C J Kolanko 1
PMCID: PMC1135836  PMID: 7619074

Abstract

The size distribution of aminoacyl-tRNA synthetase activity was investigated in cell extracts prepared from Saccharomyces cerevisiae. Bio-Gel A-5M chromatography of 105,000 g supernatants separated isoleucyl-tRNA synthetase activity into three peaks, with apparent molecular masses (Da) of about 100,000, 350,000 and 10(6) or greater. Similar results were obtained with synthetases specific for glutamic acid, serine and tyrosine. Sucrose-density-gradient centrifugation of yeast supernatants also provided evidence for the existence of synthetase complexes. These data provide the first evidence for the existence of a high-molecular-mass aminoacyl-tRNA synthetase complex in yeast, perhaps similar to those reported in higher eukaryotes.

Full text

PDF
321

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bandyopadhyay A. K., Deutscher M. P. Complex of aminoacyl-transfer RNA synthetases. J Mol Biol. 1971 Aug 28;60(1):113–122. doi: 10.1016/0022-2836(71)90451-7. [DOI] [PubMed] [Google Scholar]
  2. Brevet A., Kellermann O., Tonetti H., Waller J. P. Macromolecular complexes of aminoacyl-tRNA synthetases from eukaryotes. 2. Agarose gel-filtration behaviour of the extensively purified high-molecular-weight complex(es) of seven aminoacyl-tRNA synthetases from sheep liver. Eur J Biochem. 1979 Sep;99(3):551–558. doi: 10.1111/j.1432-1033.1979.tb13287.x. [DOI] [PubMed] [Google Scholar]
  3. Cirakoglu B., Waller J. P. Do yeast aminoacyl-tRNA synthetases exist as soluble enzymes within the cytoplasm? Eur J Biochem. 1985 Jun 3;149(2):353–361. doi: 10.1111/j.1432-1033.1985.tb08933.x. [DOI] [PubMed] [Google Scholar]
  4. Dang C. V., Dang C. V. Multienzyme complex of aminoacyl-tRNA synthetases: an essence of being eukaryotic. Biochem J. 1986 Oct 15;239(2):249–255. doi: 10.1042/bj2390249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deutscher M. P. The eucaryotic aminoacyl-tRNA synthetase complex: suggestions for its structure and function. J Cell Biol. 1984 Aug;99(2):373–377. doi: 10.1083/jcb.99.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Godar D. E., Godar D. E., Garcia V., Jacobo A., Aebi U., Yang D. C. Structural organization of the multienzyme complex of mammalian aminoacyl-tRNA synthetases. Biochemistry. 1988 Sep 6;27(18):6921–6928. doi: 10.1021/bi00418a038. [DOI] [PubMed] [Google Scholar]
  7. Harris C. L. An aminoacyl-tRNA synthetase complex in Escherichia coli. J Bacteriol. 1987 Jun;169(6):2718–2723. doi: 10.1128/jb.169.6.2718-2723.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harris C. L. High-molecular-weight forms of aminoacyl-tRNA synthetases and tRNA modification enzymes in Escherichia coli. J Bacteriol. 1990 Apr;172(4):1798–1803. doi: 10.1128/jb.172.4.1798-1803.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kellermann O., Brevet A., Tonetti H., Waller J. P. Macromolecular complexes of aminoacyl-tRNA synthetases from eukaryotes. 1. Extensive purification and characterization of the high-molecular-weight complex(es) of seven aminoacyl-tRNA synthetases from sheep liver. Eur J Biochem. 1979 Sep;99(3):541–550. doi: 10.1111/j.1432-1033.1979.tb13286.x. [DOI] [PubMed] [Google Scholar]
  10. Mirande M., Pailliez J. P., Schwencke J., Waller J. P. Sedimentation behaviour of aminoacyl-tRNA synthetases from mixed lysates of yeast and rabbit liver. Biochim Biophys Acta. 1983 Sep 14;747(1-2):71–77. doi: 10.1016/0167-4838(83)90123-1. [DOI] [PubMed] [Google Scholar]
  11. Negrutskii B. S., Deutscher M. P. A sequestered pool of aminoacyl-tRNA in mammalian cells. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3601–3604. doi: 10.1073/pnas.89.8.3601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Norcum M. T. Isolation and electron microscopic characterization of the high molecular mass aminoacyl-tRNA synthetase complex from murine erythroleukemia cells. J Biol Chem. 1989 Sep 5;264(25):15043–15051. [PubMed] [Google Scholar]
  13. Pendergast A. M., Traugh J. A. Alteration of aminoacyl-tRNA synthetase activities by phosphorylation with casein kinase I. J Biol Chem. 1985 Sep 25;260(21):11769–11774. [PubMed] [Google Scholar]
  14. Schimmel P. R., Söll D. Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs. Annu Rev Biochem. 1979;48:601–648. doi: 10.1146/annurev.bi.48.070179.003125. [DOI] [PubMed] [Google Scholar]
  15. Srere P. A., Mathews C. K. Purification of multienzyme complexes. Methods Enzymol. 1990;182:539–551. doi: 10.1016/0076-6879(90)82043-2. [DOI] [PubMed] [Google Scholar]
  16. Yang D. C., Garcia J. V., Johnson Y. D., Wahab S. Multienzyme complexes of mammalian aminoacyl-tRNA synthetases. Curr Top Cell Regul. 1985;26:325–335. doi: 10.1016/b978-0-12-152826-3.50031-0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES