Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1995 Jan 15;305(Pt 2):521–524. doi: 10.1042/bj3050521

Escherichia coli dihydrodipicolinate synthase: characterization of the imine intermediate and the product of bromopyruvate treatment by electrospray mass spectrometry

Emma B Borthwick *, Susanne J Connell †, David W Tudor †, David J Robins †, Andrea Shneier ‡, Chris Abell ‡, John R Coggins *,§
PMCID: PMC1136393  PMID: 7832769

Abstract

Simplified procedures for assaying and purifying dihydrodipicolinate synthase (EC 4.2.1.52), the first enzyme of the lysine biosynthetic pathway, have been developed and electrospray ionization m.s. has been used to observe the imine intermediate with pyruvate and to investigate the reaction of the enzyme with bromopyruvate and fluoropyruvate.

Full text

PDF
521

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK S., WRIGHT N. G. Aspartic beta-semialdehyde dehydrogenase and aspartic beta-semialdehyde. J Biol Chem. 1955 Mar;213(1):39–50. [PubMed] [Google Scholar]
  2. BLACK S., WRIGHT N. G. Homoserine dehydrogenase. J Biol Chem. 1955 Mar;213(1):51–60. [PubMed] [Google Scholar]
  3. Bonnassie S., Oreglia J., Sicard A. M. Nucleotide sequence of the dapA gene from Corynebacterium glutamicum. Nucleic Acids Res. 1990 Nov 11;18(21):6421–6421. doi: 10.1093/nar/18.21.6421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  5. Frisch D. A., Tommey A. M., Gengenbach B. G., Somers D. A. Direct genetic selection of a maize cDNA for dihydrodipicolinate synthase in an Escherichia coli dapA- auxotroph. Mol Gen Genet. 1991 Aug;228(1-2):287–293. doi: 10.1007/BF00282478. [DOI] [PubMed] [Google Scholar]
  6. Kaneko T., Hashimoto T., Kumpaisal R., Yamada Y. Molecular cloning of wheat dihydrodipicolinate synthase. J Biol Chem. 1990 Oct 15;265(29):17451–17455. [PubMed] [Google Scholar]
  7. Kimura K. Pyridine-2,6-dicarboxylic acid (dipicolinic acid) formation in Bacillus subtilis. I. Non-enzymatic formation of dipicolinic acid from pyruvate and aspartic semialdehyde. J Biochem. 1974 May;75(5):961–967. doi: 10.1093/oxfordjournals.jbchem.a130498. [DOI] [PubMed] [Google Scholar]
  8. Kumpaisal R., Hashimoto T., Yamada Y. Purification and characterization of dihydrodipicolinate synthase from wheat suspension cultures. Plant Physiol. 1987 Sep;85(1):145–151. doi: 10.1104/pp.85.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laber B., Gomis-Rüth F. X., Romão M. J., Huber R. Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization. Biochem J. 1992 Dec 1;288(Pt 2):691–695. doi: 10.1042/bj2880691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. POWELL J. F. Isolation of dipicolinic acid (pyridine-2:6-dicarboxylic acid) from spores of Bacillus megatherium. Biochem J. 1953 May;54(2):210–211. doi: 10.1042/bj0540210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richaud F., Richaud C., Ratet P., Patte J. C. Chromosomal location and nucleotide sequence of the Escherichia coli dapA gene. J Bacteriol. 1986 Apr;166(1):297–300. doi: 10.1128/jb.166.1.297-300.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shedlarski J. G., Gilvarg C. The pyruvate-aspartic semialdehyde condensing enzyme of Escherichia coli. J Biol Chem. 1970 Mar 25;245(6):1362–1373. [PubMed] [Google Scholar]
  13. Yugari Y., Gilvarg C. The condensation step in diaminopimelate synthesis. J Biol Chem. 1965 Dec;240(12):4710–4716. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES