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. 1995 Jan 1;305(Pt 1):221–224. doi: 10.1042/bj3050221

Identification of an immunodominant functional domain on human CD36 antigen using human-mouse chimaeric proteins and homologue-replacement mutagenesis.

L Daviet 1, R Buckland 1, M D Puente Navazo 1, J L McGregor 1
PMCID: PMC1136452  PMID: 7529996

Abstract

The human CD36 antigen is a multifunctional membrane glycoprotein that acts as a receptor for thrombospondin, malaria-infected erythrocytes and oxidized low-density lipoprotein, as well as being implicated in the recognition of apoptotic neutrophils by macrophages. OKM5 and other anti-CD36 monoclonal antibodies have been shown to inhibit these CD36 adhesive functions, suggesting that the monoclonal-antibody epitopes and the domains that mediate these events are closely related. Analysis of a series of chimaeric exchanges between human and mouse CD36 shows that six anti-CD36 monoclonal antibodies (OKM5, FA6-152, L103, 5F1, SM phi and 10/5) recognize epitopes within the domain comprising amino acids 155-183. A seventh monoclonal antibody (13/10) binds to another domain that spans amino acids 30-76. Homologue-replacement mutagenesis performed within the human 155-183 immunodominant sequence identifies key residues for the binding of three functional monoclonal antibodies (OKM5, FA6-152 and L103). The fact that antibodies directed against the 155-183 domain can inhibit adhesion suggests that this domain is directly involved in CD36-ligand binding.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abumrad N. A., el-Maghrabi M. R., Amri E. Z., Lopez E., Grimaldi P. A. Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation. Homology with human CD36. J Biol Chem. 1993 Aug 25;268(24):17665–17668. [PubMed] [Google Scholar]
  2. Aiken M. L., Ginsberg M. H., Byers-Ward V., Plow E. F. Effects of OKM5, a monoclonal antibody to glycoprotein IV, on platelet aggregation and thrombospondin surface expression. Blood. 1990 Dec 15;76(12):2501–2509. [PubMed] [Google Scholar]
  3. Asch A. S., Barnwell J., Silverstein R. L., Nachman R. L. Isolation of the thrombospondin membrane receptor. J Clin Invest. 1987 Apr;79(4):1054–1061. doi: 10.1172/JCI112918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berendt A. R., McDowall A., Craig A. G., Bates P. A., Sternberg M. J., Marsh K., Newbold C. I., Hogg N. The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site. Cell. 1992 Jan 10;68(1):71–81. doi: 10.1016/0092-8674(92)90207-s. [DOI] [PubMed] [Google Scholar]
  5. Calvo D., Vega M. A. Identification, primary structure, and distribution of CLA-1, a novel member of the CD36/LIMPII gene family. J Biol Chem. 1993 Sep 5;268(25):18929–18935. [PubMed] [Google Scholar]
  6. Colman P. M. Structure of antibody-antigen complexes: implications for immune recognition. Adv Immunol. 1988;43:99–132. doi: 10.1016/s0065-2776(08)60364-8. [DOI] [PubMed] [Google Scholar]
  7. Endemann G., Stanton L. W., Madden K. S., Bryant C. M., White R. T., Protter A. A. CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 1993 Jun 5;268(16):11811–11816. [PubMed] [Google Scholar]
  8. Greenwalt D. E., Lipsky R. H., Ockenhouse C. F., Ikeda H., Tandon N. N., Jamieson G. A. Membrane glycoprotein CD36: a review of its roles in adherence, signal transduction, and transfusion medicine. Blood. 1992 Sep 1;80(5):1105–1115. [PubMed] [Google Scholar]
  9. Huang M. M., Bolen J. B., Barnwell J. W., Shattil S. J., Brugge J. S. Membrane glycoprotein IV (CD36) is physically associated with the Fyn, Lyn, and Yes protein-tyrosine kinases in human platelets. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7844–7848. doi: 10.1073/pnas.88.17.7844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jin L., Fendly B. M., Wells J. A. High resolution functional analysis of antibody-antigen interactions. J Mol Biol. 1992 Aug 5;226(3):851–865. doi: 10.1016/0022-2836(92)90636-x. [DOI] [PubMed] [Google Scholar]
  11. Leung L. L., Li W. X., McGregor J. L., Albrecht G., Howard R. J. CD36 peptides enhance or inhibit CD36-thrombospondin binding. A two-step process of ligand-receptor interaction. J Biol Chem. 1992 Sep 5;267(25):18244–18250. [PubMed] [Google Scholar]
  12. McClelland A., deBear J., Yost S. C., Meyer A. M., Marlor C. W., Greve J. M. Identification of monoclonal antibody epitopes and critical residues for rhinovirus binding in domain 1 of intercellular adhesion molecule 1. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7993–7997. doi: 10.1073/pnas.88.18.7993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ockenhouse C. F., Tandon N. N., Jamieson G. A., Greenwalt D. E. Antigenic and functional differences in adhesion of Plasmodium falciparum-infected erythrocytes to human and bovine CD36. Infect Immun. 1993 May;61(5):2229–2232. doi: 10.1128/iai.61.5.2229-2232.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oquendo P., Hundt E., Lawler J., Seed B. CD36 directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Cell. 1989 Jul 14;58(1):95–101. doi: 10.1016/0092-8674(89)90406-6. [DOI] [PubMed] [Google Scholar]
  15. Savill J., Hogg N., Ren Y., Haslett C. Thrombospondin cooperates with CD36 and the vitronectin receptor in macrophage recognition of neutrophils undergoing apoptosis. J Clin Invest. 1992 Oct;90(4):1513–1522. doi: 10.1172/JCI116019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schüepp B. J., Pfister H., Clemetson K. J., Silverstein R. L., Jungi T. W. CD36-mediated signal transduction in human monocytes by anti-CD36 antibodies but not by anti-thrombospondin antibodies recognizing cell membrane-bound thrombospondin. Biochem Biophys Res Commun. 1991 Feb 28;175(1):263–270. doi: 10.1016/s0006-291x(05)81229-x. [DOI] [PubMed] [Google Scholar]
  17. Shattil S. J., Cunningham M., Hoxie J. A. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood. 1987 Jul;70(1):307–315. [PubMed] [Google Scholar]
  18. Tandon N. N., Kralisz U., Jamieson G. A. Identification of glycoprotein IV (CD36) as a primary receptor for platelet-collagen adhesion. J Biol Chem. 1989 May 5;264(13):7576–7583. [PubMed] [Google Scholar]
  19. Vega M. A., Seguí-Real B., García J. A., Calés C., Rodríguez F., Vanderkerckhove J., Sandoval I. V. Cloning, sequencing, and expression of a cDNA encoding rat LIMP II, a novel 74-kDa lysosomal membrane protein related to the surface adhesion protein CD36. J Biol Chem. 1991 Sep 5;266(25):16818–16824. [PubMed] [Google Scholar]
  20. Wyler B., Daviet L., Bortkiewicz H., Bordet J. C., McGregor J. L. Cloning of the cDNA encoding human platelet CD36: comparison to PCR amplified fragments of monocyte, endothelial and HEL cells. Thromb Haemost. 1993 Sep 1;70(3):500–505. [PubMed] [Google Scholar]

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