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. 1995 Feb 15;306(Pt 1):279–284. doi: 10.1042/bj3060279

The proteolytic processing site of the precursor of lysyl oxidase.

A D Cronshaw 1, L A Fothergill-Gilmore 1, D J Hulmes 1
PMCID: PMC1136513  PMID: 7864821

Abstract

The precise cleavage site of the N-terminal propeptide region of the precursor of lysyl oxidase has not yet been established, due to N-terminal blocking of the mature protein. Using a combination of peptide fragmentation, amino acid sequencing, time-of-flight m.s. and partial chemical unblocking procedures, it is shown that the mature form of lysyl oxidase begins at residue Asp-169 of the precursor protein (numbered according to the human sequence). The cleavage site is 28 residues to the C-terminal side of the site previously suggested on the basis of apparant molecular mass by SDS/PAGE, with the consequence that the two putative, N-linked glycosylation sites and the position of the Arg/Gln sequence polymorphism are now all in the precursor region.

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Selected References

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