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. 1995 Feb 15;306(Pt 1):305–309. doi: 10.1042/bj3060305

Purification of a phosphatidic-acid-hydrolysing phospholipase A2 from rat brain.

F J Thomson 1, M A Clark 1
PMCID: PMC1136517  PMID: 7864824

Abstract

A phosphatidic-acid-hydrolysing phospholipase A2 was purified from rat brain and characterized. This phospholipase A2 was purified by sequential cation, hydrophobic, heparin and gel-filtration chromatography. The purified protein had a mass of approximately 58 kDa as assayed by SDS/PAGE, had a pH optimum of 6.0, and was Ca(2+)-independent. This enzyme was apparently phosphatidic-acid-selective and had little measurable catalytic activity when phosphatidylcholine, phosphatidylethanolamine or diacylglycerol was used as substrate. On the basis of its physical and catalytic properties, we conclude that this phospholipase A2 is unique from those previously purified, and we speculate that it may be important for the production of the bioactive lipid lysophosphatidic acid.

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Selected References

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