Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1995 Mar 1;306(Pt 2):327–330. doi: 10.1042/bj3060327

The characterization of two diazepam binding inhibitor (DBI) transcripts in humans.

M Kolmer 1, A Rovio 1, H Alho 1
PMCID: PMC1136524  PMID: 7534063

Abstract

We have investigated the expression of diazepam binding inhibitor (DBI) (also called acyl-CoA-binding protein or endozepine) transcripts in different human tissues and tissue culture cell lines by reverse-transcriptase assisted PCR and RNase protection assay. Two different DBI transcripts capable of encoding polypeptides of 86 and 104 amino acids were detected in all the human tissues and cell lines studied. The transcript coding for the 86 amino acid DBI polypeptide was found to represent the majority of the total DBI transcript pool.

Full text

PDF
327

Images in this article

328Figure 1
on p.328
329Figure 3
on p.329
329Figure 5
on p.329

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agerberth B., Boman A., Andersson M., Jörnvall H., Mutt V., Boman H. G. Isolation of three antibacterial peptides from pig intestine: gastric inhibitory polypeptide (7-42), diazepam-binding inhibitor (32-86) and a novel factor, peptide 3910. Eur J Biochem. 1993 Sep 1;216(2):623–629. doi: 10.1111/j.1432-1033.1993.tb18182.x. [DOI] [PubMed] [Google Scholar]
  2. Alho H., Fremeau R. T., Jr, Tiedge H., Wilcox J., Bovolin P., Brosius J., Roberts J. L., Costa E. Diazepam binding inhibitor gene expression: location in brain and peripheral tissues of rat. Proc Natl Acad Sci U S A. 1988 Sep;85(18):7018–7022. doi: 10.1073/pnas.85.18.7018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ball J. A., Ghatei M. A., Sekiya K., Krausz T., Bloom S. R. Diazepam binding inhibitor-like immunoreactivity(51-70): distribution in human brain, spinal cord and peripheral tissues. Brain Res. 1989 Feb 13;479(2):300–305. doi: 10.1016/0006-8993(89)91632-6. [DOI] [PubMed] [Google Scholar]
  4. Besman M. J., Yanagibashi K., Lee T. D., Kawamura M., Hall P. F., Shively J. E. Identification of des-(Gly-Ile)-endozepine as an effector of corticotropin-dependent adrenal steroidogenesis: stimulation of cholesterol delivery is mediated by the peripheral benzodiazepine receptor. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4897–4901. doi: 10.1073/pnas.86.13.4897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Borboni P., Condorelli L., De Stefanis P., Sesti G., Lauro R. Modulation of insulin secretion by diazepam binding inhibitor and its processing products. Neuropharmacology. 1991 Dec;30(12B):1399–1403. doi: 10.1016/s0028-3908(11)80008-0. [DOI] [PubMed] [Google Scholar]
  6. Boujrad N., Hudson J. R., Jr, Papadopoulos V. Inhibition of hormone-stimulated steroidogenesis in cultured Leydig tumor cells by a cholesterol-linked phosphorothioate oligodeoxynucleotide antisense to diazepam-binding inhibitor. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5728–5731. doi: 10.1073/pnas.90.12.5728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bovolin P., Schlichting J., Miyata M., Ferrarese C., Guidotti A., Alho H. Distribution and characterization of diazepam binding inhibitor (DBI) in peripheral tissues of rat. Regul Pept. 1990 Jul 30;29(2-3):267–281. doi: 10.1016/0167-0115(90)90089-f. [DOI] [PubMed] [Google Scholar]
  8. Chen Z. W., Agerberth B., Gell K., Andersson M., Mutt V., Ostenson C. G., Efendić S., Barros-Söderling J., Persson B., Jörnvall H. Isolation and characterization of porcine diazepam-binding inhibitor, a polypeptide not only of cerebral occurrence but also common in intestinal tissues and with effects on regulation of insulin release. Eur J Biochem. 1988 Jun 1;174(2):239–245. doi: 10.1111/j.1432-1033.1988.tb14088.x. [DOI] [PubMed] [Google Scholar]
  9. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  10. Costa E., Guidotti A. Diazepam binding inhibitor (DBI): a peptide with multiple biological actions. Life Sci. 1991;49(5):325–344. doi: 10.1016/0024-3205(91)90440-m. [DOI] [PubMed] [Google Scholar]
  11. DeBernardi M. A., Crowe R. R., Mocchetti I., Shows T. B., Eddy R. L., Costa E. Chromosomal localization of the human diazepam binding inhibitor gene. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6561–6565. doi: 10.1073/pnas.85.17.6561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gray P. W., Glaister D., Seeburg P. H., Guidotti A., Costa E. Cloning and expression of cDNA for human diazepam binding inhibitor, a natural ligand of an allosteric regulatory site of the gamma-aminobutyric acid type A receptor. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7547–7551. doi: 10.1073/pnas.83.19.7547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Guidotti A., Forchetti C. M., Corda M. G., Konkel D., Bennett C. D., Costa E. Isolation, characterization, and purification to homogeneity of an endogenous polypeptide with agonistic action on benzodiazepine receptors. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3531–3535. doi: 10.1073/pnas.80.11.3531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Knudsen J., Højrup P., Hansen H. O., Hansen H. F., Roepstorff P. Acyl-CoA-binding protein in the rat. Purification, binding characteristics, tissue concentrations and amino acid sequence. Biochem J. 1989 Sep 1;262(2):513–519. doi: 10.1042/bj2620513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knudsen J., Mandrup S., Rasmussen J. T., Andreasen P. H., Poulsen F., Kristiansen K. The function of acyl-CoA-binding protein (ACBP)/diazepam binding inhibitor (DBI). Mol Cell Biochem. 1993 Jun 9;123(1-2):129–138. doi: 10.1007/BF01076484. [DOI] [PubMed] [Google Scholar]
  17. Kolmer M., Alho H., Costa E., Pani L. Cloning and tissue-specific functional characterization of the promoter of the rat diazepam binding inhibitor, a peptide with multiple biological actions. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8439–8443. doi: 10.1073/pnas.90.18.8439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kolmer M., Roos C., Tirronen M., Myöhänen S., Alho H. Tissue-specific expression of the diazepam-binding inhibitor in Drosophila melanogaster: cloning, structure, and localization of the gene. Mol Cell Biol. 1994 Oct;14(10):6983–6995. doi: 10.1128/mcb.14.10.6983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lihrmann I., Plaquevent J. C., Tostivint H., Raijmakers R., Tonon M. C., Conlon J. M., Vaudry H. Frog diazepam-binding inhibitor: peptide sequence, cDNA cloning, and expression in the brain. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6899–6903. doi: 10.1073/pnas.91.15.6899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mandrup S., Hummel R., Ravn S., Jensen G., Andreasen P. H., Gregersen N., Knudsen J., Kristiansen K. Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. A typical housekeeping gene family. J Mol Biol. 1992 Dec 5;228(3):1011–1022. doi: 10.1016/0022-2836(92)90888-q. [DOI] [PubMed] [Google Scholar]
  21. Marquardt H., Todaro G. J., Shoyab M. Complete amino acid sequences of bovine and human endozepines. Homology with rat diazepam binding inhibitor. J Biol Chem. 1986 Jul 25;261(21):9727–9731. [PubMed] [Google Scholar]
  22. Mikkelsen J., Højrup P., Nielsen P. F., Roepstorff P., Knudsen J. Amino acid sequence of acyl-CoA-binding protein from cow liver. Biochem J. 1987 Aug 1;245(3):857–861. doi: 10.1042/bj2450857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mocchetti I., Einstein R., Brosius J. Putative diazepam binding inhibitor peptide: cDNA clones from rat. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7221–7225. doi: 10.1073/pnas.83.19.7221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ostenson C. G., Ahrén B., Karlsson S., Sandberg E., Efendic S. Effects of porcine diazepam-binding inhibitor on insulin and glucagon secretion in vitro from the rat endocrine pancreas. Regul Pept. 1990 Jul 30;29(2-3):143–151. doi: 10.1016/0167-0115(90)90077-a. [DOI] [PubMed] [Google Scholar]
  25. Owens G. P., Sinha A. K., Sikela J. M., Hahn W. E. Sequence and expression of the murine diazepam binding inhibitor. Brain Res Mol Brain Res. 1989 Nov;6(2-3):101–108. doi: 10.1016/0169-328x(89)90043-0. [DOI] [PubMed] [Google Scholar]
  26. Papadopoulos V., Berkovich A., Krueger K. E., Costa E., Guidotti A. Diazepam binding inhibitor and its processing products stimulate mitochondrial steroid biosynthesis via an interaction with mitochondrial benzodiazepine receptors. Endocrinology. 1991 Sep;129(3):1481–1488. doi: 10.1210/endo-129-3-1481. [DOI] [PubMed] [Google Scholar]
  27. Papadopoulos V., Guarneri P., Kreuger K. E., Guidotti A., Costa E. Pregnenolone biosynthesis in C6-2B glioma cell mitochondria: regulation by a mitochondrial diazepam binding inhibitor receptor. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5113–5117. doi: 10.1073/pnas.89.11.5113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rose T. M., Schultz E. R., Sasaki G. C., Kolattukudy P. E., Shoyab M. Nucleotide sequence and genomic structure of duck acyl-CoA binding protein/diazepam-binding inhibitor: co-localization with S-acyl fatty acid synthase thioesterase. DNA Cell Biol. 1994 Jun;13(6):669–678. doi: 10.1089/dna.1994.13.669. [DOI] [PubMed] [Google Scholar]
  29. Rose T. M., Schultz E. R., Todaro G. J. Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11287–11291. doi: 10.1073/pnas.89.23.11287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tenhunen J., Salminen M., Lundström K., Kiviluoto T., Savolainen R., Ulmanen I. Genomic organization of the human catechol O-methyltransferase gene and its expression from two distinct promoters. Eur J Biochem. 1994 Aug 1;223(3):1049–1059. doi: 10.1111/j.1432-1033.1994.tb19083.x. [DOI] [PubMed] [Google Scholar]
  32. Webb N. R., Rose T. M., Malik N., Marquardt H., Shoyab M., Todaro G. J., Lee D. C. Bovine and human cDNA sequences encoding a putative benzodiazepine receptor ligand. DNA. 1987 Feb;6(1):71–79. doi: 10.1089/dna.1987.6.71. [DOI] [PubMed] [Google Scholar]
  33. Yanagibashi K., Ohno Y., Kawamura M., Hall P. F. The regulation of intracellular transport of cholesterol in bovine adrenal cells: purification of a novel protein. Endocrinology. 1988 Oct;123(4):2075–2082. doi: 10.1210/endo-123-4-2075. [DOI] [PubMed] [Google Scholar]
  34. van Kammen D. P., Guidotti A., Kelley M. E., Gurklis J., Guarneri P., Gilbertson M. W., Yao J. K., Peters J., Costa E. CSF diazepam binding inhibitor and schizophrenia: clinical and biochemical relationships. Biol Psychiatry. 1993 Oct 15;34(8):515–522. doi: 10.1016/0006-3223(93)90193-h. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES