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. 1990 Jan 1;265(1):39–44. doi: 10.1042/bj2650039

Identification and characterization of somatostatin receptors in neonatal rat long bones.

C Bruns 1, M M Dietl 1, J M Palacios 1, J Pless 1
PMCID: PMC1136611  PMID: 1967933

Abstract

Somatostatin (somatotropin release inhibiting factor; SRIF) has widespread functions as a modulator of neural activity as well as of endocrine and exocrine secretion. In the present paper, the binding characteristics of somatostatin receptors have been investigated in rat long bones using the stable analogue, 125I-SDZ 204-090, as a ligand. Binding studies revealed the presence of a single class of high-affinity binding sites for 125I-SDZ 204-090 on cells prepared from neonatal rat long bones with an equilibrium dissociation constant (KD) of 70.1 +/- 8.2 pM (n = 3). An excellent correlation was found between the ability of various somatostatin analogues to inhibit growth hormone in pituitary cells and to displace the binding of 125I-SDZ 204-090 to the bone cell preparation, indicating that the receptors are very similar, if not identical. The localization of the somatostatin-binding sites was examined by autoradiography after labelling in vitro and in vivo. The binding sites were shown by both procedures to be selectively localized to the metaphysis of rat long bones. The labelling experiments in vivo indicate that these receptors can be reached in the living animal by circulating somatostatin analogues. In addition, the analogue SMS 201-995 inhibited the forskolin-stimulated adenylate cyclase activity in bone cell suspensions. These results suggest that somatostatin could be an important regulatory factor in bone metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer W., Briner U., Doepfner W., Haller R., Huguenin R., Marbach P., Petcher T. J., Pless SMS 201-995: a very potent and selective octapeptide analogue of somatostatin with prolonged action. Life Sci. 1982 Sep 13;31(11):1133–1140. doi: 10.1016/0024-3205(82)90087-x. [DOI] [PubMed] [Google Scholar]
  2. Benoit R., Böhlen P., Ling N., Briskin A., Esch F., Brazeau P., Ying S. Y., Guillemin R. Presence of somatostatin-28-(1-12) in hypothalamus and pancreas. Proc Natl Acad Sci U S A. 1982 Feb;79(3):917–921. doi: 10.1073/pnas.79.3.917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borgeat P., Labrie F., Drouin J., Bélanger A., Immer H., Sestanj K., Nelson V., Götz M., Schally A. V., Coy D. H. Inhibition of adenosine 3',5'-monophosphate accumulation in anterior pituitary gland in vitro by growth hormone-release inhibiting hormone. Biochem Biophys Res Commun. 1974 Feb 27;56(4):1052–1059. doi: 10.1016/s0006-291x(74)80295-0. [DOI] [PubMed] [Google Scholar]
  4. Brazeau P., Vale W., Burgus R., Ling N., Butcher M., Rivier J., Guillemin R. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science. 1973 Jan 5;179(4068):77–79. doi: 10.1126/science.179.4068.77. [DOI] [PubMed] [Google Scholar]
  5. Brownstein M., Arimura A., Sato H., Schally A. V., Kizer J. S. The regional distribution of somatostatin in the rat brain. Endocrinology. 1975 Jun;96(6):1456–1461. doi: 10.1210/endo-96-6-1456. [DOI] [PubMed] [Google Scholar]
  6. Carter R. F., Bitar K. N., Zfass A. M., Makhlouf G. M. Inhibition of VIP-stimulated intestinal secretion and cyclic AMP production by somatostatin in the rat. Gastroenterology. 1978 Apr;74(4):726–730. [PubMed] [Google Scholar]
  7. Efendic S., Grill V., Luft R. Inhibition by somatostatin of glucose induced 3':5'-monophsophate (cyclic AMP) accumulation and insulin release in isolated pancreatic islets of the rat. FEBS Lett. 1975 Jul 15;55(1):131–133. doi: 10.1016/0014-5793(75)80977-x. [DOI] [PubMed] [Google Scholar]
  8. Johansson O., Madsen K. Stimulative effect of somatostatin on cell proliferation in cultured chondrocytes. Med Biol. 1987;65(1):57–60. [PubMed] [Google Scholar]
  9. Koch B. D., Blalock J. B., Schonbrunn A. Characterization of the cyclic AMP-independent actions of somatostatin in GH cells. I. An increase in potassium conductance is responsible for both the hyperpolarization and the decrease in intracellular free calcium produced by somatostatin. J Biol Chem. 1988 Jan 5;263(1):216–225. [PubMed] [Google Scholar]
  10. Marie P. J., Hott M., Durand D. Somatostatin infusion inhibits the stimulatory effect of testosterone on endosteal bone formation in the mouse. Metabolism. 1988 May;37(5):429–435. doi: 10.1016/0026-0495(88)90042-x. [DOI] [PubMed] [Google Scholar]
  11. Markstein R., Enz A., Vigouret J. M., Jaton A., Closse A., Briner U., Gull P. Biochemical, behavioural, and endocrine effects of CK 204-933, a novel 8 beta-ergolene. J Neural Transm. 1987;69(3-4):179–199. doi: 10.1007/BF01244340. [DOI] [PubMed] [Google Scholar]
  12. Maurer R., Reubi J. C. Somatostatin receptors in the adrenal. Mol Cell Endocrinol. 1986 Apr;45(1):81–90. doi: 10.1016/0303-7207(86)90086-9. [DOI] [PubMed] [Google Scholar]
  13. Nicholson G. C., Moseley J. M., Sexton P. M., Mendelsohn F. A., Martin T. J. Abundant calcitonin receptors in isolated rat osteoclasts. Biochemical and autoradiographic characterization. J Clin Invest. 1986 Aug;78(2):355–360. doi: 10.1172/JCI112584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nilsson A., Isgaard J., Lindahl A., Dahlström A., Skottner A., Isaksson O. G. Regulation by growth hormone of number of chondrocytes containing IGF-I in rat growth plate. Science. 1986 Aug 1;233(4763):571–574. doi: 10.1126/science.3523759. [DOI] [PubMed] [Google Scholar]
  15. Oliver J. R. Inhibition of calcium uptake by somatostatin in isolated rat islets of Langerhans. Endocrinology. 1976 Sep;99(3):910–913. doi: 10.1210/endo-99-3-910. [DOI] [PubMed] [Google Scholar]
  16. Patel Y. C., Srikant C. B. Somatostatin mediation of adenohypophysial secretion. Annu Rev Physiol. 1986;48:551–567. doi: 10.1146/annurev.ph.48.030186.003003. [DOI] [PubMed] [Google Scholar]
  17. Reichlin S. Somatostatin (second of two parts). N Engl J Med. 1983 Dec 22;309(25):1556–1563. doi: 10.1056/NEJM198312223092506. [DOI] [PubMed] [Google Scholar]
  18. Reichlin S. Somatostatin. N Engl J Med. 1983 Dec 15;309(24):1495–1501. doi: 10.1056/NEJM198312153092406. [DOI] [PubMed] [Google Scholar]
  19. Reubi J. C., Cortès R., Maurer R., Probst A., Palacios J. M. Distribution of somatostatin receptors in the human brain: an autoradiographic study. Neuroscience. 1986 Jun;18(2):329–346. doi: 10.1016/0306-4522(86)90158-2. [DOI] [PubMed] [Google Scholar]
  20. Reubi J. C., Maurer R. Autoradiographic mapping of somatostatin receptors in the rat central nervous system and pituitary. Neuroscience. 1985 Aug;15(4):1183–1193. doi: 10.1016/0306-4522(85)90261-1. [DOI] [PubMed] [Google Scholar]
  21. Reubi J. C. New specific radioligand for one subpopulation of brain somatostatin receptors. Life Sci. 1985 May 13;36(19):1829–1836. doi: 10.1016/0024-3205(85)90155-9. [DOI] [PubMed] [Google Scholar]
  22. Reubi J. C., Perrin M. H., Rivier J. E., Vale W. High affinity binding sites for a somatostatin-28 analog in rat brain. Life Sci. 1981 May 11;28(19):2191–2198. doi: 10.1016/0024-3205(81)90628-7. [DOI] [PubMed] [Google Scholar]
  23. Sakamoto C., Goldfine I. D., Williams J. A. The somatostatin receptor on isolated pancreatic acinar cell plasma membranes. Identification of subunit structure and direct regulation by cholecystokinin. J Biol Chem. 1984 Aug 10;259(15):9623–9627. [PubMed] [Google Scholar]
  24. Silve C., Lieberherr M., Garabedian M., Guillozo H., Grosse B., Thil C. L., Balsan S. Somatostatin and vitamin D3 metabolites in rat calvarium: in vitro evidence for physiological interaction. Endocrinology. 1981 Nov;109(5):1454–1462. doi: 10.1210/endo-109-5-1454. [DOI] [PubMed] [Google Scholar]
  25. Srikant C. B., Patel Y. C. Characterization of pituitary membrane receptors for somatostatin in the rat. Endocrinology. 1982 Jun;110(6):2138–2144. doi: 10.1210/endo-110-6-2138. [DOI] [PubMed] [Google Scholar]
  26. Srikant C. B., Patel Y. C. Somatostatin receptors: identification and characterization in rat brain membranes. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3930–3934. doi: 10.1073/pnas.78.6.3930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Steiner A. L., Kipnis D. M., Utiger R., Parker C. Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate. Proc Natl Acad Sci U S A. 1969 Sep;64(1):367–373. doi: 10.1073/pnas.64.1.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vale W., Brazeau P., Rivier C., Brown M., Boss B., Rivier J., Burgus R., Ling N., Guillemin R. Somatostatin. Recent Prog Horm Res. 1975;31:365–397. doi: 10.1016/b978-0-12-571131-9.50014-1. [DOI] [PubMed] [Google Scholar]
  29. Weiss R. E., Reddi A. H., Nimni M. E. Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells. Calcif Tissue Int. 1981;33(4):425–430. doi: 10.1007/BF02409466. [DOI] [PubMed] [Google Scholar]

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