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. 1995 May 15;308(Pt 1):77–81. doi: 10.1042/bj3080077

Identification of a new surfactant protein A binding protein at the cell membrane of rat type II pneumocytes.

P A Stevens 1, H Wissel 1, D Sieger 1, V Meienreis-Sudau 1, B Rüstow 1
PMCID: PMC1136845  PMID: 7755591

Abstract

Antibodies against a type II pneumocyte component were developed by an auto-anti-idiotypic approach using surfactant-associated protein A (SP-A) as the immunogen. The antibodies recognize an SP-A-binding protein (approximately 170-200 kDa under non-reducing conditions, 55 kDa under reducing conditions) on the cell membrane of rat type II pneumocytes. One of the antibodies competes with SP-A for binding to type II cells. In immunization assays in vitro, with this antibody as the antigen, anti-SP-A antibodies have been generated. The SP-A-binding cell membrane protein recognized by this auto-anti-idiotypic antibody may be involved in the interaction of extracellular SP-A with the type II pneumocyte and may play a role in the regulation of alveolar surfactant metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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