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. 1990 Jan 15;265(2):415–419. doi: 10.1042/bj2650415

Mitochondria have Fe(III) receptors.

J Weaver 1, H Zhan 1, S Pollack 1
PMCID: PMC1136902  PMID: 2154188

Abstract

Recent work has provided new evidence that ATP is the major constituent of the low-Mr iron pool in the reticulocyte. The interaction of the iron complex of ATP with mitochondria was investigated in the present experiments. When ATP-Fe3+ was incubated with mitochondria, Fe3+, free of ATP, bound with high affinity to Fe3+ receptors on the mitochondria. The binding was saturable and reversible. Iron which was complexed to PPi, nitrilotriacetate, citrate, ADP and GTP also showed saturable binding to mitochondria; Fe3+ complexed to AMP bound non-specifically, as did Fe2+/ascorbate complexed to AMP bound non-specifically, as did Fe2+/ascorbate and Fe2+/dithionite.

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Selected References

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  1. AASA R., MALMSTROEM B. G., SALTMAN P. THE SPECIFIC BINDING OF IRON(III) AND COPPER(II) TO TRANSFERRIN AND CONALBUMIN. Biochim Biophys Acta. 1963 Sep 24;75:203–222. doi: 10.1016/0006-3002(63)90599-7. [DOI] [PubMed] [Google Scholar]
  2. Bakkeren D. L., de Jeu-Jaspars C. M., van der Heul C., van Eijk H. G. Analysis of iron-binding components in the low molecular weight fraction of rat reticulocyte cytosol. Int J Biochem. 1985;17(8):925–930. doi: 10.1016/0020-711x(85)90177-6. [DOI] [PubMed] [Google Scholar]
  3. Bartlett G. R. Iron nucleotides in human and rat red cells. Biochem Biophys Res Commun. 1976 Jun 21;70(4):1063–1070. doi: 10.1016/0006-291x(76)91010-x. [DOI] [PubMed] [Google Scholar]
  4. Bomford A., Young S., Williams R. Intracellular forms of iron during transferrin iron uptake by mitogen-stimulated human lymphocytes. Br J Haematol. 1986 Mar;62(3):487–494. doi: 10.1111/j.1365-2141.1986.tb02960.x. [DOI] [PubMed] [Google Scholar]
  5. Borová J., Ponka P., Neuwirt J. Study of intracellular iron distribution in rabbit reticulocytes with normal and inhibited heme synthesis. Biochim Biophys Acta. 1973 Aug 17;320(1):143–156. doi: 10.1016/0304-4165(73)90174-8. [DOI] [PubMed] [Google Scholar]
  6. Boulard M., Delin M., Najean Y., Beutler E. Identification and purification of a new non-heme, non-ferritin iron protein. Proc Soc Exp Biol Med. 1972 Apr;139(4):1379–1384. doi: 10.3181/00379727-139-36367. [DOI] [PubMed] [Google Scholar]
  7. Cowart R. E., Kojima N., Bates G. W. The exchange of Fe3+ between acetohydroxamic acid and transferrin. Spectrophotometric evidence for a mixed ligand complex. J Biol Chem. 1982 Jul 10;257(13):7560–7565. [PubMed] [Google Scholar]
  8. Eguchi L. A., Saltman P. The aerobic reduction of Fe(III) complexes by hemoglobin and myoglobin. J Biol Chem. 1984 Dec 10;259(23):14337–14338. [PubMed] [Google Scholar]
  9. Fernandez-Pol J. A. Isolation and characterization of a siderophore-like growth factor from mutants of SV40-transformed cells adapted to picolinic acid. Cell. 1978 Jul;14(3):489–499. doi: 10.1016/0092-8674(78)90235-0. [DOI] [PubMed] [Google Scholar]
  10. Hussein S., Hantke K., Braun V. Citrate-dependent iron transport system in Escherichia coli K-12. Eur J Biochem. 1981 Jul;117(2):431–437. doi: 10.1111/j.1432-1033.1981.tb06357.x. [DOI] [PubMed] [Google Scholar]
  11. Jones R. L., Grady R. W., Sorette M. P., Cerami A. Host-associated iron transfer factor in normal humans and patients with transfusion siderosis. J Lab Clin Med. 1986 May;107(5):431–438. [PubMed] [Google Scholar]
  12. KEBERLE H. THE BIOCHEMISTRY OF DESFERRIOXAMINE AND ITS RELATION TO IRON METABOLISM. Ann N Y Acad Sci. 1964 Oct 7;119:758–768. doi: 10.1111/j.1749-6632.1965.tb54077.x. [DOI] [PubMed] [Google Scholar]
  13. Konopka K. Differential effects of metal-binding agents on the uptake of iron from transferrin by isolated rat liver mitochondria. FEBS Lett. 1978 Aug 15;92(2):308–312. doi: 10.1016/0014-5793(78)80776-5. [DOI] [PubMed] [Google Scholar]
  14. Konopka K., Romslo I. Studies on the mechanism of pyrophosphate-mediated uptake of iron from transferrin by isolated rat-liver mitochondria. Eur J Biochem. 1981 Jul;117(2):239–244. doi: 10.1111/j.1432-1033.1981.tb06328.x. [DOI] [PubMed] [Google Scholar]
  15. Konopka K., Romslo I. Uptake of iron from transferrin by isolated rat-liver mitochondria mediated by phosphate compounds. Eur J Biochem. 1980 Jun;107(2):433–439. doi: 10.1111/j.1432-1033.1980.tb06048.x. [DOI] [PubMed] [Google Scholar]
  16. Konopka K., Szotor M. Determination of iron in the acid-soluble fraction of human erythrocytes. Acta Haematol. 1972;47(3):157–163. doi: 10.1159/000208510. [DOI] [PubMed] [Google Scholar]
  17. LaCross D. M., Linder M. C. Synthesis of rat muscle ferritins and function in iron metabolism of heart and diaphragm. Biochim Biophys Acta. 1980 Nov 17;633(1):45–55. doi: 10.1016/0304-4165(80)90036-7. [DOI] [PubMed] [Google Scholar]
  18. Mansour M. M., Schulert A. R., Glasser S. R. Mechanism of placental iron transfer in the rat. Am J Physiol. 1972 Jun;222(6):1628–1633. doi: 10.1152/ajplegacy.1972.222.6.1628. [DOI] [PubMed] [Google Scholar]
  19. Meyers N. L., Brewer G. J., Oelshlegel F. J., Jr Iron-ATP, a by-product of acid extraction of whole blood or red blood cells. Biochim Biophys Acta. 1973 Sep 14;320(2):397–405. doi: 10.1016/0304-4165(73)90321-8. [DOI] [PubMed] [Google Scholar]
  20. Milsom J. P., Batey R. G. The mechanism of hepatic iron uptake from native and denatured transferrin and its subcellular metabolism in the liver cell. Biochem J. 1979 Jul 15;182(1):117–125. doi: 10.1042/bj1820117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mulligan M., Althaus B., Linder M. C. Non-ferritin, non-heme iron pools in rat tissues. Int J Biochem. 1986;18(9):791–798. doi: 10.1016/0020-711x(86)90055-8. [DOI] [PubMed] [Google Scholar]
  22. Neilands J. B. Iron absorption and transport in microorganisms. Annu Rev Nutr. 1981;1:27–46. doi: 10.1146/annurev.nu.01.070181.000331. [DOI] [PubMed] [Google Scholar]
  23. Nilsen T., Romslo I. Iron uptake and heme synthesis by isolated rat liver mitochondria. Diferric transferrin as iron donor and the effect of pyrophosphate. Biochim Biophys Acta. 1985 Oct 17;842(2-3):162–169. doi: 10.1016/0304-4165(85)90198-9. [DOI] [PubMed] [Google Scholar]
  24. Nilsen T., Romslo I. Pyrophosphate as a ligand for delivery of iron to isolated rat-liver mitochondria. Biochim Biophys Acta. 1984 Jul 27;766(1):233–239. doi: 10.1016/0005-2728(84)90236-6. [DOI] [PubMed] [Google Scholar]
  25. Nilsen T., Romslo I. Transferrin as a donor of iron to mitochondria. Effect of pyrophosphate and relationship to mitochondrial metabolism and heme synthesis. Biochim Biophys Acta. 1984 Dec 20;802(3):448–453. doi: 10.1016/0304-4165(84)90363-5. [DOI] [PubMed] [Google Scholar]
  26. Nunez M. T., Glass J., Robinson S. H. Mobilization of iron from the plasma membrane of the murine reticulocyte. The role of ferritin. Biochim Biophys Acta. 1978 May 4;509(1):170–180. doi: 10.1016/0005-2736(78)90017-2. [DOI] [PubMed] [Google Scholar]
  27. Pickart L., Thaler M. M. Growth-modulating tripeptide (glycylhistidyllysine): association with copper and iron in plasma, and stimulation of adhesiveness and growth of hepatoma cells in culture by tripeptide-metal ion complexes. J Cell Physiol. 1980 Feb;102(2):129–139. doi: 10.1002/jcp.1041020205. [DOI] [PubMed] [Google Scholar]
  28. Pippard M. J., Johnson D. K., Finch C. A. Hepatocyte iron kinetics in the rat explored with an iron chelator. Br J Haematol. 1982 Oct;52(2):211–224. doi: 10.1111/j.1365-2141.1982.tb03883.x. [DOI] [PubMed] [Google Scholar]
  29. Pollack S., Campana T. Early events in guinea pig reticulocyte iron uptake. Biochim Biophys Acta. 1981 Apr 3;673(4):366–373. doi: 10.1016/0304-4165(81)90468-2. [DOI] [PubMed] [Google Scholar]
  30. Pollack S., Campana T., Weaver J. Low molecular weight iron in guinea pig reticulocytes. Am J Hematol. 1985 May;19(1):75–84. doi: 10.1002/ajh.2830190110. [DOI] [PubMed] [Google Scholar]
  31. Pollack S., Weaver J. Iron release from transferrin: synergistic interaction between adenosine triphosphate and an ammonium sulfate fraction of hemolysate. J Lab Clin Med. 1986 Nov;108(5):411–414. [PubMed] [Google Scholar]
  32. Primosigh J. V., Thomas E. D. Studies on the partition of iron in bone marrow cells. J Clin Invest. 1968 Jul;47(7):1473–1482. doi: 10.1172/JCI105841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Russell L. M., Holmes R. K. Initial characterization of the ferric iron transport system of Corynebacterium diphtheriae. J Bacteriol. 1983 Sep;155(3):1439–1442. doi: 10.1128/jb.155.3.1439-1442.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Ulvik R., Prante P. H., Koller M. E., Romslo I. Transferrin and iron uptake by isolated rat liver mitochondria. Scand J Clin Lab Invest. 1976 Oct;36(6):539–546. doi: 10.3109/00365517609054476. [DOI] [PubMed] [Google Scholar]
  35. Weaver J., Pollack S. Low-Mr iron isolated from guinea pig reticulocytes as AMP-Fe and ATP-Fe complexes. Biochem J. 1989 Aug 1;261(3):787–792. doi: 10.1042/bj2610787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. White G. P., Bailey-Wood R., Jacobs A. The effect of chelating agents on cellular iron metabolism. Clin Sci Mol Med. 1976 Mar;50(3):145–152. doi: 10.1042/cs0500145. [DOI] [PubMed] [Google Scholar]
  37. Zhan H., Pollack S., Weaver J. Hemolysates reduce iron released from transferrin. Am J Hematol. 1989 Jul;31(3):203–207. doi: 10.1002/ajh.2830310311. [DOI] [PubMed] [Google Scholar]

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