Fig. 1.

Identification of the Targeted Antigen Recognized by mAb 9B8.

A. Purification of antigen recognized by mAb 9B8 from the cell lysate of SPCA-1 sphere cells. Protein were electrophoresed (right panel) and immunoblotted with mAb 9B8 (left panel). B. A protein band excised from SDS-PAGE was identified as HSP90α using mass spectrometry and n Mascot database analysis. C. Identification of mAb 9B8-binding antigen by Immunoprecipitation-Western blot. Immunoprecipitation using purified mAb 9B8 or a commercial antibody against HSP90α was followed by Western blotting for HSP90. Results confirmed the interaction between the HSP90α antigen and mAb 9B8 in a protein band.