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[Preprint]. 2024 Aug 24:2024.08.23.609423. [Version 1] doi: 10.1101/2024.08.23.609423

Figure 3. Activation of β-catenin partially rescues zG morphology, but not function, in Wnt2b KO mice.

Figure 3.

a. Treatment protocol with 0.06% lithium chloride (LiCl) or water from birth to 6 weeks of age.

b. Representative images and quantification from 6-week-old adrenals stained for DAB2 (gray, n=11 WT, n=7 KO, n=8 KO+LiCl), Gαq (magenta, n=10 WT, n=7 KO, n=8 KO+LiCl), β-catenin (β-cat, red, n=10 WT, n=6 KO, n=8 KO+LiCl) and CYP11B2 (green, n=6 WT, n=8 KO, n=6 KO+LiCl) mice. Positive cells were quantified and normalized to nuclei (DAPI, blue) in the cortex. Scale bars: 10μm. One-way ANOVA with Tukey’s post-test. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Data are represented as mean ± SEM.

c, d. Quantification of (c) aldosterone levels from (female, n=5 WT, n=6 KO, n=3 KO+LiCl; male, n=6 WT, n=4 KO, n=5 KO+LiCl) and (d) plasma renin (female, n=5 WT, n=6 KO, n=3 KO+LiCl; male, n=6 WT, n=4 KO, n=6 KO+LiCl) mice. Data are represented as mean ± SEM. One-way ANOVA with Tukey’s post-test. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001. Data are represented as mean ± SEM.