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. 1994 Nov 15;304(Pt 1):189–193. doi: 10.1042/bj3040189

Synthesis and characterization of 3H-labelled tetrahydrobiopterin.

E R Werner 1, M Schmid 1, G Werner-Felmayer 1, B Mayer 1, H Wachter 1
PMCID: PMC1137470  PMID: 7528005

Abstract

We synthesized [3'-3H]-5,6,7,8-tetrahydrobiopterin from [8,5'-3H]guanosine 5'-triphosphate ([8,5'-3H]GTP) using GTP cyclohydrolase (EC 3.5.4.16), 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase (EC 1.1.1.153). After purification by cation-exchange h.p.l.c. a solution of radiochemically pure (> 95%) [3'-3H]-5,6,7,8-tetrahydrobiopterin with a specific activity of 9.2 Ci/mmol was obtained. The product proved well suited for studying the binding of tetrahydrobiopterin to nitric-oxide synthase.

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Selected References

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