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. 1994 Dec 1;304(Pt 2):347–351. doi: 10.1042/bj3040347

Neutrophil collagenase (MMP-8) cleaves at the aggrecanase site E373-A374 in the interglobular domain of cartilage aggrecan.

A J Fosang 1, K Last 1, P J Neame 1, G Murphy 1, V Knäuper 1, H Tschesche 1, C E Hughes 1, B Caterson 1, T E Hardingham 1
PMCID: PMC1137499  PMID: 7998967

Abstract

Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E373-A374 'aggrecanase' site in the interglobular domain of aggrecan. The time course of digestion in vitro showed that MMP-8 cleaved initially at N341-F342, the predominant metalloproteinase site, before cleaving at the E373-A374 site. A synthetic peptide, IPENFFG, inhibited cleavage at E373-A374 but not N341-F342 in vitro, indicating that the E373-A374 sequence was a less preferred site for MMP-8 cleavage than N341-F342. IPENFFG also inhibited release of A374 RGSVI fragments from cartilage in explant culture, suggesting that a metalloproteinase cleaved at the aggrecanase site in situ. The possibility remains that 'aggrecanase' may be a metalloproteinase in cartilage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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