Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1993 Dec 1;296(Pt 2):287–290. doi: 10.1042/bj2960287

The human phenolsulphotransferase polymorphism is determined by the level of expression of the enzyme protein.

A L Jones 1, R C Roberts 1, M W Coughtrie 1
PMCID: PMC1137690  PMID: 8257413

Abstract

We have examined the expression of platelet phenolsulphotransferase (PST) in 60 individuals. Using an antibody which recognizes both forms of PST present in man (P-PST and M-PST), we determined that the polymorphism of platelet P-PST activity is determined by the level of expression of the enzyme protein. The implications for susceptibility to adverse drug reactions and chemical carcinogenesis are discussed.

Full text

PDF
287

Images in this article

288Figure 1
on p.288
289Figure 2
on p.289
289Figure 3
on p.289

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. J., Weinshilboum R. M., Phillips S. F., Broughton D. D. Human platelet phenol sulphotransferase: assay procedure, substrate and tissue correlations. Clin Chim Acta. 1981 Mar 5;110(2-3):157–167. doi: 10.1016/0009-8981(81)90345-4. [DOI] [PubMed] [Google Scholar]
  2. Bonham Carter S. M., Rein G., Glover V., Sandler M., Caldwell J. Human platelet phenolsulphotransferase M and P: substrate specificities and correlation with in vivo sulphoconjugation of paracetamol and salicylamide. Br J Clin Pharmacol. 1983 Mar;15(3):323–330. doi: 10.1111/j.1365-2125.1983.tb01506.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borthwick E. B., Burchell A., Coughtrie M. W. Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase. Biochem J. 1993 Feb 1;289(Pt 3):719–725. doi: 10.1042/bj2890719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butler P. R., Anderson R. J., Venton D. L. Human platelet phenol sulfotransferase: partial purification and detection of two forms of the enzyme. J Neurochem. 1983 Sep;41(3):630–639. doi: 10.1111/j.1471-4159.1983.tb04788.x. [DOI] [PubMed] [Google Scholar]
  5. Campbell N. R., Sundaram R. S., Werness P. G., Van Loon J., Weinshilboum R. M. Sulfate and methyldopa metabolism: metabolite patterns and platelet phenol sulfotransferase activity. Clin Pharmacol Ther. 1985 Mar;37(3):308–315. doi: 10.1038/clpt.1985.45. [DOI] [PubMed] [Google Scholar]
  6. Campbell N. R., Van Loon J. A., Weinshilboum R. M. Human liver phenol sulfotransferase: assay conditions, biochemical properties and partial purification of isozymes of the thermostable form. Biochem Pharmacol. 1987 May 1;36(9):1435–1446. doi: 10.1016/0006-2952(87)90108-0. [DOI] [PubMed] [Google Scholar]
  7. Carter S. M., Glover V., Sandler M., Gillman P. K., Bridges P. K. Human platelet phenolsulphotransferase: separate control of the two forms and activity range in depressive illness. Clin Chim Acta. 1981 Dec 24;117(3):333–344. doi: 10.1016/0009-8981(81)90121-2. [DOI] [PubMed] [Google Scholar]
  8. Coughtrie M. W., Burchell B., Leakey J. E., Hume R. The inadequacy of perinatal glucuronidation: immunoblot analysis of the developmental expression of individual UDP-glucuronosyltransferase isoenzymes in rat and human liver microsomes. Mol Pharmacol. 1988 Dec;34(6):729–735. [PubMed] [Google Scholar]
  9. Falany C. N. Molecular enzymology of human liver cytosolic sulfotransferases. Trends Pharmacol Sci. 1991 Jul;12(7):255–259. doi: 10.1016/0165-6147(91)90566-b. [DOI] [PubMed] [Google Scholar]
  10. Foldes A., Meek J. L. Rat brain phenolsulfotransferase: partial purification and some properties. Biochim Biophys Acta. 1973 Dec 19;327(2):365–374. doi: 10.1016/0005-2744(73)90419-1. [DOI] [PubMed] [Google Scholar]
  11. Glatt H., Henschler R., Frank H., Seidel A., Yang C., Abu-Shqara E., Harvey R. G. Sulfotransferase-mediated mutagenicity of 1-hydroxymethylpyrene and 4H-cyclopenta[def]chrysen-4-ol and its enhancement by chloride anions. Carcinogenesis. 1993 Apr;14(4):599–602. doi: 10.1093/carcin/14.4.599. [DOI] [PubMed] [Google Scholar]
  12. Heroux J. A., Falany C. N., Roth J. A. Immunological characterization of human phenol sulfotransferase. Mol Pharmacol. 1989 Jul;36(1):29–33. [PubMed] [Google Scholar]
  13. Heroux J. A., Roth J. A. Physical characterization of a monoamine-sulfating form of phenol sulfotransferase from human platelets. Mol Pharmacol. 1988 Aug;34(2):194–199. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lai C. C., Miller E. C., Miller J. A., Liem A. Initiation of hepatocarcinogenesis in infant male B6C3F1 mice by N-hydroxy-2-aminofluorene or N-hydroxy-2-acetylaminofluorene depends primarily on metabolism to N-sulfooxy-2-aminofluorene and formation of DNA-(deoxyguanosin-8-yl)-2-aminofluorene adducts. Carcinogenesis. 1987 Mar;8(3):471–478. doi: 10.1093/carcin/8.3.471. [DOI] [PubMed] [Google Scholar]
  17. Price R. A., Spielman R. S., Lucena A. L., Van Loon J. A., Maidak B. L., Weinshilboum R. M. Genetic polymorphism for human platelet thermostable phenol sulfotransferase (TS PST) activity. Genetics. 1989 Aug;122(4):905–914. doi: 10.1093/genetics/122.4.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rein G., Glover V., Sandler M. Multiple forms of phenolsulphotransferase in human tissues: selective inhibition by dichloronitrophenol. Biochem Pharmacol. 1982 May 15;31(10):1893–1897. doi: 10.1016/0006-2952(82)90493-2. [DOI] [PubMed] [Google Scholar]
  19. Reiter C., Mwaluko G., Dunnette J., Van Loon J., Weinshilboum R. Thermolabile and thermostable human platelet phenol sulfotransferase. Substrate specificity and physical separation. Naunyn Schmiedebergs Arch Pharmacol. 1983 Sep;324(2):140–147. doi: 10.1007/BF00497020. [DOI] [PubMed] [Google Scholar]
  20. Reiter C., Weinshilboum R. Platelet phenol sulfotransferase activity: correlation with sulfate conjugation of acetaminophen. Clin Pharmacol Ther. 1982 Nov;32(5):612–621. doi: 10.1038/clpt.1982.211. [DOI] [PubMed] [Google Scholar]
  21. Reveley A. M., Bonham Carter S. M., Reveley M. A., Sandler M. A genetic study of platelet phenolsulphotransferase activity in normal and schizophrenic twins. J Psychiatr Res. 1982;17(3):303–307. doi: 10.1016/0022-3956(82)90009-7. [DOI] [PubMed] [Google Scholar]
  22. Sharp S., Barker E. V., Coughtrie M. W., Lowenstein P. R., Hume R. Immunochemical characterisation of a dehydroepiandrosterone sulfotransferase in rats and humans. Eur J Biochem. 1993 Feb 1;211(3):539–548. doi: 10.1111/j.1432-1033.1993.tb17580.x. [DOI] [PubMed] [Google Scholar]
  23. Sothmann M. S., Blaney J., Woulfe T., Donahue-Fuhrman S., Lefever K., Gustafson A. B., Murthy V. S. Plasma free and sulfoconjugated catecholamines during sustained exercise. J Appl Physiol (1985) 1990 Feb;68(2):452–456. doi: 10.1152/jappl.1990.68.2.452. [DOI] [PubMed] [Google Scholar]
  24. Sundaram R. S., Van Loon J. A., Tucker R., Weinshilboum R. M. Sulfation pharmacogenetics: correlation of human platelet and small intestinal phenol sulfotransferase. Clin Pharmacol Ther. 1989 Nov;46(5):501–509. doi: 10.1038/clpt.1989.178. [DOI] [PubMed] [Google Scholar]
  25. Surh Y. J., Kwon H., Tannenbaum S. R. Sulfotransferase-mediated activation of 4-hydroxy- and 3,4-dihydroxy-3,4-dihydrocyclopenta[c,d]pyrene, major metabolites of cyclopenta[c,d]pyrene. Cancer Res. 1993 Mar 1;53(5):1017–1022. [PubMed] [Google Scholar]
  26. Weinshilboum R. Sulfotransferase pharmacogenetics. Pharmacol Ther. 1990;45(1):93–107. doi: 10.1016/0163-7258(90)90010-y. [DOI] [PubMed] [Google Scholar]
  27. Young W. F., Jr, Laws E. R., Jr, Sharbrough F. W., Weinshilboum R. M. Human phenol sulfotransferase: correlation of brain and platelet activities. J Neurochem. 1985 Apr;44(4):1131–1137. doi: 10.1111/j.1471-4159.1985.tb08734.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES