Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1994 Mar 1;298(Pt 2):377–383. doi: 10.1042/bj2980377

Calcium promotes membrane association of reticulocyte 15-lipoxygenase.

A Watson 1, F J Doherty 1
PMCID: PMC1137950  PMID: 8135744

Abstract

The reticulocyte 15-lipoxygenase (linoleate:oxygen oxidoreductase, EC 1.13.11.12) is implicated in oxidative damage to reticulocyte mitochondria before their elimination by degradation during maturation to the erythrocyte. A proportion of the 15-lipoxygenase sediments with the mitochondrial-rich stromal fraction of density-gradient-fractionated rabbit reticulocytes suggesting a physical association with mitochondria before their elimination. Ca2+ promotes binding of reticulocyte 15-lipoxygenase to isolated rat liver and reticulocyte mitochondria and 15-lipoxygenase-mediated lipid peroxidation of mitochondrial lipids and free linoleic acid. Association of reticulocyte 15-lipoxygenase with isolated mitochondria is not simply a consequence of Ca(2+)-induced swelling, but implies that Ca2+ mediates translocation of soluble lipoxygenase to mitochondrial membranes. Therefore, Ca2+ may have an important physiological role in the regulation of 15-lipoxygenase-mediated targeting of reticulocyte mitochondria for degradation.

Full text

PDF
377

Images in this article

380Figure 2
on p.380
380Figure 3
on p.380
381Figure 4
on p.381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burrall B. A., Cheung M., Chiu A., Goetzl E. J. Enzymatic properties of the 15-lipoxygenase of human cultured keratinocytes. J Invest Dermatol. 1988 Oct;91(4):294–297. doi: 10.1111/1523-1747.ep12475450. [DOI] [PubMed] [Google Scholar]
  2. Doherty F. J., Osborn N. U., Wassell J. A., Heggie P. E., Laszlo L., Mayer R. J. Ubiquitin-protein conjugates accumulate in the lysosomal system of fibroblasts treated with cysteine proteinase inhibitors. Biochem J. 1989 Oct 1;263(1):47–55. doi: 10.1042/bj2630047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ford R. E., Ou C. N., Ellefson R. D. Assay for erythrocyte uroporphyrinogen I synthase activity, with porphobilinogen as substrate. Clin Chem. 1980 Jul;26(8):1182–1185. [PubMed] [Google Scholar]
  4. Gordon P. B., Tolleshaug H., Seglen P. O. Use of digitonin extraction to distinguish between autophagic-lysosomal sequestration and mitochondrial uptake of [14C]sucrose in hepatocytes. Biochem J. 1985 Dec 15;232(3):773–780. doi: 10.1042/bj2320773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kuhn H., Belkner J., Wiesner R., Brash A. R. Oxygenation of biological membranes by the pure reticulocyte lipoxygenase. J Biol Chem. 1990 Oct 25;265(30):18351–18361. [PubMed] [Google Scholar]
  6. Kühn H., Belkner J., Wiesner R. Subcellular distribution of lipoxygenase products in rabbit reticulocyte membranes. Eur J Biochem. 1990 Jul 20;191(1):221–227. doi: 10.1111/j.1432-1033.1990.tb19113.x. [DOI] [PubMed] [Google Scholar]
  7. Magnani M., Serafini G., Stocchi V. Effects of Ca2+ and lipoxygenase inhibitors on hexokinase degradation in rabbit reticulocytes. Mol Cell Biochem. 1989 Jan 23;85(1):3–7. doi: 10.1007/BF00223508. [DOI] [PubMed] [Google Scholar]
  8. Miller D. K., Gillard J. W., Vickers P. J., Sadowski S., Léveillé C., Mancini J. A., Charleson P., Dixon R. A., Ford-Hutchinson A. W., Fortin R. Identification and isolation of a membrane protein necessary for leukotriene production. Nature. 1990 Jan 18;343(6255):278–281. doi: 10.1038/343278a0. [DOI] [PubMed] [Google Scholar]
  9. Rapoport S. M., Schewe T. The maturational breakdown of mitochondria in reticulocytes. Biochim Biophys Acta. 1986 Dec 22;864(3-4):471–495. doi: 10.1016/0304-4157(86)90006-7. [DOI] [PubMed] [Google Scholar]
  10. Rapoport S. M., Schewe T., Wiesner R., Halangk W., Ludwig P., Janicke-Höhne M., Tannert C., Hiebsch C., Klatt D. The lipoxygenase of reticulocytes. Purification, characterization and biological dynamics of the lipoxygenase; its identity with the respiratory inhibitors of the reticulocyte. Eur J Biochem. 1979 Jun 1;96(3):545–561. doi: 10.1111/j.1432-1033.1979.tb13068.x. [DOI] [PubMed] [Google Scholar]
  11. Rapoport S., Schmidt J., Prehn S. Fe-dependent formation of a protein that makes mitochondria lipoxygenase-susceptible during maturation of reticulocytes. FEBS Lett. 1986 Mar 17;198(1):109–112. doi: 10.1016/0014-5793(86)81194-2. [DOI] [PubMed] [Google Scholar]
  12. Rapoport S., Schmidt J., Prehn S. Maturation of rabbit reticulocytes: susceptibility of mitochondria to ATP-dependent proteolysis is determined by the maturational state of reticulocyte. FEBS Lett. 1985 Apr 22;183(2):370–374. doi: 10.1016/0014-5793(85)80812-7. [DOI] [PubMed] [Google Scholar]
  13. Rouzer C. A., Ford-Hutchinson A. W., Morton H. E., Gillard J. W. MK886, a potent and specific leukotriene biosynthesis inhibitor blocks and reverses the membrane association of 5-lipoxygenase in ionophore-challenged leukocytes. J Biol Chem. 1990 Jan 25;265(3):1436–1442. [PubMed] [Google Scholar]
  14. Schewe T., Halangk W., Hiebsch C., Rapoport S. M. A lipoxygenase in rabbit reticulocytes which attacks phospholipids and intact mitochondria. FEBS Lett. 1975 Dec 1;60(1):149–152. doi: 10.1016/0014-5793(75)80439-x. [DOI] [PubMed] [Google Scholar]
  15. Soberman R. J., Harper T. W., Betteridge D., Lewis R. A., Austen K. F. Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes. J Biol Chem. 1985 Apr 10;260(7):4508–4515. [PubMed] [Google Scholar]
  16. Thiele B. J., Belkner J., Andree H., Rapoport T. A., Rapoport S. M. Synthesis of non-globin proteins in rabbit-erythroid cells. Synthesis of a lipoxygenase in reticulocytes. Eur J Biochem. 1979 Jun 1;96(3):563–569. doi: 10.1111/j.1432-1033.1979.tb13070.x. [DOI] [PubMed] [Google Scholar]
  17. Wiesner R., Kasüschke A., Kühn H., Anton M., Schewe T. Oxygenation of mitochondrial membranes by the reticulocyte lipoxygenase. Action on monoamine oxidase activities A and B. Biochim Biophys Acta. 1989 Nov 17;986(1):11–17. doi: 10.1016/0005-2736(89)90266-6. [DOI] [PubMed] [Google Scholar]
  18. Wong A., Hwang S. M., Cook M. N., Hogaboom G. K., Crooke S. T. Interactions of 5-lipoxygenase with membranes: studies on the association of soluble enzyme with membranes and alterations in enzyme activity. Biochemistry. 1988 Sep 6;27(18):6763–6769. doi: 10.1021/bi00418a018. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES