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. 1994 Feb 15;298(Pt 1):223–229. doi: 10.1042/bj2980223

Phospholipid-transfer proteins and their mRNAs in developing rat lung and in alveolar type-II cells.

J J Batenburg 1, B C Ossendorp 1, G T Snoek 1, K W Wirtz 1, M Houweling 1, R H Elfring 1
PMCID: PMC1138005  PMID: 8129723

Abstract

Gene expression of non-specific lipid-transfer protein (nsL-TP; identical with sterol carrier protein 2) and phosphatidylinositol-transfer protein (PI-TP) was investigated in developing rat lung. During the late prenatal period (between days 17 and 22) there is a 7-fold increase in the level of nsL-TP and a 2-fold rise in that of PI-TP. The prenatal increases in the levels of nsL-TP and PI-TP are accompanied by parallel increases in the levels of their mRNAs, indicating pretranslational regulation. Compared with whole lung, isolated alveolar type-II cells are enriched in nsL-TP and its mRNA, but not in PI-TP and its mRNA. The observation that the levels of nsL-TP and its mRNA in rat lung show a pronounced increase in the period of accelerated surfactant formation, together with the observation that the surfactant-producing type-II cells are enriched in nsL-TP and its mRNA, suggest that nsL-TP plays a role in the metabolism of pulmonary surfactant.

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