Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1994 Apr 1;299(Pt 1):165–170. doi: 10.1042/bj2990165

Acyl-CoA-binding protein (ACBP) can mediate intermembrane acyl-CoA transport and donate acyl-CoA for beta-oxidation and glycerolipid synthesis.

J T Rasmussen 1, N J Faergeman 1, K Kristiansen 1, J Knudsen 1
PMCID: PMC1138036  PMID: 8166635

Abstract

The dissociation constants for octanoyl-CoA, dodecanoyl-CoA and hexadecanoyl-CoA binding to acyl-CoA-binding protein (ACBP) were determined by using titration microcalorimetry. The KD values obtained, (0.24 +/- 0.02) x 10(-6) M, (0.65 +/- 0.2) x 10(-8) M and (0.45 +/- 0.2) x 10(-13) M respectively, were much lower than expected. ACBP was able to extract hexadecanoyl-CoA from phosphatidylcholine membranes immobilized on a nitrocellulose membrane. The acyl-CoA/ACBP complex formed was able to transport acyl-CoA to mitochondria or microsomes in suspension, or to microsomes immobilized on a nitrocellulose membrane, and to donate them to beta-oxidation or glycerolipid synthesis in mitochondria or microsomes, respectively.

Full text

PDF
165

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen K. V., Ludvigsen S., Mandrup S., Knudsen J., Poulsen F. M. The secondary structure in solution of acyl-coenzyme A binding protein from bovine liver using 1H nuclear magnetic resonance spectroscopy. Biochemistry. 1991 Nov 5;30(44):10654–10663. doi: 10.1021/bi00108a008. [DOI] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Benz R. Porin from bacterial and mitochondrial outer membranes. CRC Crit Rev Biochem. 1985;19(2):145–190. doi: 10.3109/10409238509082542. [DOI] [PubMed] [Google Scholar]
  4. De Pinto V., Ludwig O., Krause J., Benz R., Palmieri F. Porin pores of mitochondrial outer membranes from high and low eukaryotic cells: biochemical and biophysical characterization. Biochim Biophys Acta. 1987 Nov 19;894(2):109–119. doi: 10.1016/0005-2728(87)90180-0. [DOI] [PubMed] [Google Scholar]
  5. Freitag H., Neupert W., Benz R. Purification and characterisation of a pore protein of the outer mitochondrial membrane from Neurospora crassa. Eur J Biochem. 1982 Apr;123(3):629–636. doi: 10.1111/j.1432-1033.1982.tb06578.x. [DOI] [PubMed] [Google Scholar]
  6. Hach M., Pedersen S. N., Börchers T., Højrup P., Knudsen J. Determination by photoaffinity labelling of the hydrophobic part of the binding site for acyl-CoA esters on acyl-CoA-binding protein from bovine liver. Biochem J. 1990 Oct 1;271(1):231–236. doi: 10.1042/bj2710231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Knudsen J., Højrup P., Hansen H. O., Hansen H. F., Roepstorff P. Acyl-CoA-binding protein in the rat. Purification, binding characteristics, tissue concentrations and amino acid sequence. Biochem J. 1989 Sep 1;262(2):513–519. doi: 10.1042/bj2620513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knudsen J., Mandrup S., Rasmussen J. T., Andreasen P. H., Poulsen F., Kristiansen K. The function of acyl-CoA-binding protein (ACBP)/diazepam binding inhibitor (DBI). Mol Cell Biochem. 1993 Jun 9;123(1-2):129–138. doi: 10.1007/BF01076484. [DOI] [PubMed] [Google Scholar]
  9. Knudsen J., Nielsen M. Diazepam-binding inhibitor: a neuropeptide and/or an acyl-CoA ester binding protein? Biochem J. 1990 Feb 1;265(3):927–929. doi: 10.1042/bj2650927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kragelund B. B., Andersen K. V., Madsen J. C., Knudsen J., Poulsen F. M. Three-dimensional structure of the complex between acyl-coenzyme A binding protein and palmitoyl-coenzyme A. J Mol Biol. 1993 Apr 20;230(4):1260–1277. doi: 10.1006/jmbi.1993.1240. [DOI] [PubMed] [Google Scholar]
  11. Li Q., Yamamoto N., Morisawa S., Inoue A. Fatty acyl-CoA binding activity of the nuclear thyroid hormone receptor. J Cell Biochem. 1993 Apr;51(4):458–464. doi: 10.1002/jcb.2400510411. [DOI] [PubMed] [Google Scholar]
  12. Mandrup S., Hummel R., Ravn S., Jensen G., Andreasen P. H., Gregersen N., Knudsen J., Kristiansen K. Acyl-CoA-binding protein/diazepam-binding inhibitor gene and pseudogenes. A typical housekeeping gene family. J Mol Biol. 1992 Dec 5;228(3):1011–1022. doi: 10.1016/0022-2836(92)90888-q. [DOI] [PubMed] [Google Scholar]
  13. Mandrup S., Højrup P., Kristiansen K., Knudsen J. Gene synthesis, expression in Escherichia coli, purification and characterization of the recombinant bovine acyl-CoA-binding protein. Biochem J. 1991 Jun 15;276(Pt 3):817–823. doi: 10.1042/bj2760817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mandrup S., Jepsen R., Skøtt H., Rosendal J., Højrup P., Kristiansen K., Knudsen J. Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast. Biochem J. 1993 Mar 1;290(Pt 2):369–374. doi: 10.1042/bj2900369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mannella C. A., Frank J. Negative staining characteristics of arrays of mitochondrial pore protein: use of correspondence analysis to classify different staining patterns. Ultramicroscopy. 1984;13(1-2):93–102. doi: 10.1016/0304-3991(84)90060-3. [DOI] [PubMed] [Google Scholar]
  16. McGarry J. D., Brown N. F., Inthanousay P. P., Park D. I., Cook B. A., Foster D. W. Insights into the topography of mitochondrial carnitine palmitoyltransferase gained from the use of proteases. Prog Clin Biol Res. 1992;375:47–61. [PubMed] [Google Scholar]
  17. Mikkelsen J., Højrup P., Nielsen P. F., Roepstorff P., Knudsen J. Amino acid sequence of acyl-CoA-binding protein from cow liver. Biochem J. 1987 Aug 1;245(3):857–861. doi: 10.1042/bj2450857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mikkelsen J., Knudsen J. Acyl-CoA-binding protein from cow. Binding characteristics and cellular and tissue distribution. Biochem J. 1987 Dec 15;248(3):709–714. doi: 10.1042/bj2480709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moon A., Rhead W. J. Complementation analysis of fatty acid oxidation disorders. J Clin Invest. 1987 Jan;79(1):59–64. doi: 10.1172/JCI112808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murthy M. S., Pande S. V. Malonyl-CoA binding site and the overt carnitine palmitoyltransferase activity reside on the opposite sides of the outer mitochondrial membrane. Proc Natl Acad Sci U S A. 1987 Jan;84(2):378–382. doi: 10.1073/pnas.84.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nikawa J., Tanabe T., Ogiwara H., Shiba T., Numa S. Inhibitory effects of long-chain acyl coenzyme A analogues on rat liver acetyl coenzyme A carboxylase. FEBS Lett. 1979 Jun 15;102(2):223–226. doi: 10.1016/0014-5793(79)80005-8. [DOI] [PubMed] [Google Scholar]
  22. Pande S. V. Reversal by CoA of palmityl-CoA inhibition of long chain acyl-CoA synthetase activity. Biochim Biophys Acta. 1973 Apr 13;306(1):15–20. doi: 10.1016/0005-2760(73)90202-6. [DOI] [PubMed] [Google Scholar]
  23. Pfanner N., Orci L., Glick B. S., Amherdt M., Arden S. R., Malhotra V., Rothman J. E. Fatty acyl-coenzyme A is required for budding of transport vesicles from Golgi cisternae. Cell. 1989 Oct 6;59(1):95–102. doi: 10.1016/0092-8674(89)90872-6. [DOI] [PubMed] [Google Scholar]
  24. Prentki M., Vischer S., Glennon M. C., Regazzi R., Deeney J. T., Corkey B. E. Malonyl-CoA and long chain acyl-CoA esters as metabolic coupling factors in nutrient-induced insulin secretion. J Biol Chem. 1992 Mar 25;267(9):5802–5810. [PubMed] [Google Scholar]
  25. Rasmussen J. T., Börchers T., Knudsen J. Comparison of the binding affinities of acyl-CoA-binding protein and fatty-acid-binding protein for long-chain acyl-CoA esters. Biochem J. 1990 Feb 1;265(3):849–855. doi: 10.1042/bj2650849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rasmussen J. T., Rosendal J., Knudsen J. Interaction of acyl-CoA binding protein (ACBP) on processes for which acyl-CoA is a substrate, product or inhibitor. Biochem J. 1993 Jun 15;292(Pt 3):907–913. doi: 10.1042/bj2920907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rosendal J., Ertbjerg P., Knudsen J. Characterization of ligand binding to acyl-CoA-binding protein. Biochem J. 1993 Mar 1;290(Pt 2):321–326. doi: 10.1042/bj2900321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Webb N. R., Rose T. M., Malik N., Marquardt H., Shoyab M., Todaro G. J., Lee D. C. Bovine and human cDNA sequences encoding a putative benzodiazepine receptor ligand. DNA. 1987 Feb;6(1):71–79. doi: 10.1089/dna.1987.6.71. [DOI] [PubMed] [Google Scholar]
  29. Wise B. C., Kuo J. F. Modes of inhibition by acylcarnitines, adriamycin and trifluoperazine of cardiac phospholipid-sensitive calcium-dependent protein kinase. Biochem Pharmacol. 1983 Apr 1;32(7):1259–1265. doi: 10.1016/0006-2952(83)90280-0. [DOI] [PubMed] [Google Scholar]
  30. Woldegiorgis G., Yousufzai S. Y., Shrago E. Studies on the interaction of palmitoyl coenzyme A with the adenine nucleotide translocase. J Biol Chem. 1982 Dec 25;257(24):14783–14787. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES