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. 1994 May 1;299(Pt 3):679–682. doi: 10.1042/bj2990679

Rat liver mitochondrial ADP-ribose pyrophosphatase in the matrix space with low Km for free ADP-ribose.

D Bernet 1, R M Pinto 1, M J Costas 1, J Canales 1, J C Cameselle 1
PMCID: PMC1138074  PMID: 8192656

Abstract

A study involving markers of subcellular and submitochondrial fractions, gradient centrifugation, latency measurements and extraction with digitonin, demonstrates the association of a specific ADP-ribose pyrophosphatase with rat liver mitochondria and its localization in the matrix space. The enzyme hydrolyses ADP-ribose to AMP, with a Km of 2-3 microM. The results support the occurrence of a specific turnover pathway for free ADP-ribose and its relevance in mitochondria.

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Selected References

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